Method and apparatus for immobilizing cells to treat wastewater

A technology for treating wastewater and wastewater, which is applied in the fields of chemical instruments and methods, biological water/sewage treatment, etc., which are fixed on or in biological cells, and can solve the problems of high organic solvent content, difficult selection of flora, and high operating costs. To achieve the effect of improving tolerance and degradation ability, increasing oxidation reaction rate, and increasing contact area

Inactive Publication Date: 2005-11-23
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the wastewater discharged from the production of phenolic resins and plasticizers has complex components, high content of COD and phenol, and high content of organic solvents
This kind of wastewater cannot (easily) survive in it, so most of them use organic solvent extraction to make the phenol content below 200mg/L, and then use biological methods to treat it, but the phenol recovered by the extraction method cannot be directly reused due to the large amount of impurities in it. , the operating cost is high, and the waste water is returned to the secondary pollution problem
Some people use immobi

Method used

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  • Method and apparatus for immobilizing cells to treat wastewater
  • Method and apparatus for immobilizing cells to treat wastewater
  • Method and apparatus for immobilizing cells to treat wastewater

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0036] Example 1

[0037] The method of increasing the content of plasticizer production wastewater in the culture solution is adopted to domesticate, and gradually improve the tolerance and degradation ability of white rot fungus Polyporus versicolor NG04010. The white rot fungus Versicolor Versicolor NG04010 was cultured for 7 days to collect hyphae. The culture method was a conventional method, and the culture medium was potato mash mixed with wheat bran.

[0038] Pseudomonas fluorescens ATCC11172 was cultured for 3 days, and the sludge was collected by centrifugation. The culture method was a conventional method. The medium was malic acid 10g / L, (NH 4 ) 2 SO 4 2g / L and yeast extract 1g / L.

[0039] Mix the collected hyphae and mycelium at a ratio of 5:1 to obtain a mixture, and then stir the mixture into 50-100 times (weight ratio to the mixture) of discarded Rhizopus stolonifer ATCC14037 mycelium , Mix thoroughly, then add 10% polyvinyl alcohol (PVA) solution equal to the weig...

Example Embodiment

[0041] Example 2

[0042] The method of increasing the content of landfill leachate in the culture solution is adopted to domesticate, and gradually improve the tolerance and degradation ability of the white rot fungus Pleurotus pulmonarius NG 0412. The domesticated white-rot fungus Pleurotus pulmonarius (Pleurotus pulmonarius NG 0412) was solid-cultured for 7 days to collect hyphae. The culture method was a conventional method, and the medium was potato puree mixed with wheat bran.

[0043] Nitrosomonas europaea IFO14298 was cultured for 7 days, and the sludge was collected by centrifugation. The culture method is conventional. The culture medium contains 0.3g NaCl and MgSO per liter of water. 4 ·7H 2 O 0.14g, FeSO 4 ·7H 2 O 0.03g, (NH 4 ) 2 SO 4 0.6g, KH 2 PO 4 2g.

[0044] Mix the collected mycelium and mycelium at a ratio of 3:1 to obtain a mixture, and then stir the mixture into 50-100 times (weight ratio to the mixture) of discarded Trichoderma viride (Trichoderma viride AS ...

Example Embodiment

[0046] Example 3

[0047] The method of increasing the content of phenolic resin production wastewater in the culture solution is adopted to domesticate, and gradually improve the tolerance and degradation ability of the white rot fungus Phanerochaete Chrysosporium ME-446. The white rot fungus (PChrysosporium ME-446) was cultured for 7 days to collect the hyphae. The culture method was a conventional method, and the medium was potato mash mixed with wheat bran.

[0048] Rhodococcus erythropolis NG0402 was cultured for 3 days, and the sludge was collected by centrifugation. The culture method is conventional, and the medium is glucose 20g·L -1 , Protein building 20g·L -1 , Yeast extract 10g·L -1 , K 2 HPO 4 2g·L -1 , MgSO 4 ·7H 2 O 0.5g·L -1 , PH7.0-7.2.

[0049] Mix the collected mycelium and mycelium in a ratio of 7:1 to obtain a mixture, and then stir the mixture into 50-100 times (weight ratio to the mixture) of the discarded Aspergillus ochraceus (Aspergillus ochraceus NG0401)...

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Abstract

The invention discloses a method and apparatus for immobilizing cells to treat wastewater wherein abandoned mycelium is used as a carrying agent for co-immobilization of white-rot fungi and bacteria, the enzyme system in the bacterium group is employed for coupled catalytic degradation of heterocyclic compounds and phenol in the waste water at the presence of oxygen supply. The device includes a reactor with moving sieving board and an oxygen supplying device.

Description

technical field [0001] The invention belongs to the field of wastewater treatment, and relates to a method for treating wastewater containing high-concentration phenol and organic solvents by using immobilized cells, and also relates to a device used in the method. Background technique [0002] At present, the wastewater discharged from the production of phenolic resin and plasticizer has complex components, high content of COD and phenol, and high content of organic solvents. This kind of wastewater cannot (easily) survive in it, so most of them use organic solvent extraction to make the phenol content below 200mg / L, and then use biological methods to treat it, but the phenol recovered by the extraction method cannot be directly reused due to the large amount of impurities in it. , the operating cost is high, and the waste water is returned to the secondary pollution problem. Some people use immobilized large edible fungus mycelium to treat phenol-containing wastewater, bu...

Claims

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Application Information

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IPC IPC(8): C02F3/34
Inventor 范伟平欧阳平凯吴晟旻沈珈琦
Owner NANJING UNIV OF TECH
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