Corn protein high F value oligopeptide production method

A technology of corn protein and production method, which is applied in protein food processing, protein food ingredients, food science, etc., can solve the problems of cumbersome operation, chemical pollution, and low enzymatic hydrolysis efficiency, and achieve good selectivity, easy enzymatic decomposition, and improved The effect of extraction rate

Active Publication Date: 2006-02-15
吉林省长吉图开发建设现代农业投资有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Zein is insoluble in water, if it is not solubilized by appropriate methods, and it is directly used for enzymatic hydrolysis to produce high F value oligopeptides, the enzymatic hydrolysis eff

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0013] Example 1

[0014] 1. Corn syrup degreasing and odor removal treatment

[0015] Supercritical CO 2 Fluid extraction, extraction pressure 10MPa, temperature 35℃, time 0.5 hours, CO 2 The flow rate is 15L / hour.

[0016] 2. Extraction of Zein

[0017] Supercritical CO 2 The zein powder after fluid extraction is used as a raw material, and the zein solution is extracted for 2 hours under the conditions of an ethanol concentration of 60%, a mass ratio of corn oxen powder and ethanol solution of 1:8, an extraction temperature of 50°C, and a pH value of 8.0. Centrifuge the zein solution at 3000 rpm for 5 minutes to obtain the supernatant. Dilute the supernatant with distilled water to an ethanol concentration of 30% and turbidity. When turbidity occurs, adjust the pH to 6.5 with a 4 mol HCI solution. A saturated sodium chloride solution of 2% by volume promotes the precipitation of gliadin. Centrifugal separation at 4000 rpm for 5 min. The zein precipitate is separated. The precip...

Example Embodiment

[0026] Example 2

[0027] 1. Corn syrup degreasing and odor removal treatment

[0028] Supercritical CO 2 Fluid extraction, extraction pressure 20MPa, temperature 45℃, time 1 hour, CO 2 The flow rate is 20L / hour.

[0029] 2. Extraction of Zein

[0030] Supercritical CO 2The zein powder after fluid extraction is used as a raw material, and the zein solution is extracted for 2 hours under the conditions of an ethanol concentration of 70%, a mass ratio of corn oxen powder and ethanol solution of 1:9, an extraction temperature of 55°C, and a pH value of 8.5. The following is the same as in Example 1.

[0031] 3. Lubricant treatment

[0032] Add an appropriate amount of 25°C drinking water to the zein powder, stir evenly, and leave it for 15 minutes to make the zein powder fully absorb water and ensure that its water content is 20%.

[0033] 4. High temperature and high pressure extrusion processing

[0034] Preheat the twin-screw extruder and set the temperature to 130°C. When the tem...

Example Embodiment

[0039] Example 3

[0040] 1. Corn yellow powder degreasing and deodorizing treatment

[0041] Supercritical CO 2 Fluid extraction, extraction pressure 30MPa, temperature 45℃, time 1.5 hours, CO 2 The flow rate is 25L / hour.

[0042] 2. Extraction of Zein

[0043] Supercritical CO 2 The zein powder after fluid extraction is used as the raw material, and the zein solution is extracted for 2 hours under the conditions of 80% ethanol concentration, 1:10 mass ratio of corn oxen powder and ethanol solution, extraction temperature of 60°C, and pH value of 9.0. The following is the same as in Example 1.

[0044] 3. Lubricant treatment

[0045] Add an appropriate amount of 25°C drinking water to the zein powder, stir evenly, and leave it for 15 minutes to make the zein powder fully absorb water and ensure that its water content is 25%.

[0046] 4. High temperature and high pressure extrusion processing

[0047] Preheat the twin-screw extruder and set the temperature to 140℃. When the temper...

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PUM

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Abstract

The preparation method for high-H-value oligopeptide from zein comprises, using corn powder as material, hypercritical CO2 liquid extracting to remove fat and smelly materials in said powder and extract zein; adding some water to the zein; taking extruding and modifying treatment at given temperature and pressure to increase its solubility; using two proleases for two-step enzymolysis to zein at given substrate concentration, enzyme concentration, pH value, and time condition; then, separating, purifying, drying, smashing and obtaining the product with F value more than 20.

Description

technical field [0001] The invention belongs to health food processing technology. Background technique [0002] In the amino acid mixture, the molar ratio of branched chain amino acid (BCAA for short) to aromatic amino acid (AAA for short) is called Fischer, or F value for short. High F value oligopeptide is a low molecular weight physiologically active peptide with special physiological functions formed after protease acts on protein. It is a nutritional supplement and food ingredient for patients with liver diseases. Modern medical and nutritional studies have proved that high F-value oligopeptides can inhibit hepatic encephalopathy, improve the protein nutritional status of liver patients, and improve the body's anti-fatigue ability. It is reported that in 1990, Shin-ya T. et al. degraded zein with basophilic protease and actinase to develop a non-bitter high F-value oligopeptide with a yield of 56%, an F value of 20.47, and an AAA content of 1.86. %. In 1992, Shuji A...

Claims

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Application Information

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IPC IPC(8): C12P21/00A23J1/12A23J3/34
Inventor 王大为张艳荣
Owner 吉林省长吉图开发建设现代农业投资有限公司
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