Corn protein high F value oligopeptide production method

[0013] Example 1

[0014] 1. Corn syrup degreasing and odor removal treatment

[0015] Supercritical CO 2 Fluid extraction, extraction pressure 10MPa, temperature 35℃, time 0.5 hours, CO 2 The flow rate is 15L/hour.

[0016] 2. Extraction of Zein

[0017] Supercritical CO 2 The zein powder after fluid extraction is used as a raw material, and the zein solution is extracted for 2 hours under the conditions of an ethanol concentration of 60%, a mass ratio of corn oxen powder and ethanol solution of 1:8, an extraction temperature of 50°C, and a pH value of 8.0. Centrifuge the zein solution at 3000 rpm for 5 minutes to obtain the supernatant. Dilute the supernatant with distilled water to an ethanol concentration of 30% and turbidity. When turbidity occurs, adjust the pH to 6.5 with a 4 mol HCI solution. A saturated sodium chloride solution of 2% by volume promotes the precipitation of gliadin. Centrifugal separation at 4000 rpm for 5 min. The zein precipitate is separated. The precipitate is washed twice with pure water, then dried in vacuum and pulverized. Zein powder.

[0018] 3. Lubricant treatment

[0019] Add the zein powder to drinking water at 25°C, stir it evenly, and leave it for 15 minutes to make the zein powder fully absorb water and ensure that its water content is 15%.

[0020] 4. High temperature and high pressure extrusion processing

[0021] Preheat the single-screw extruder and set the temperature to 120°C. When the die temperature reaches 120°C, start the extruder for extrusion operation, and control the feeding speed to 15kg/hour. During the extrusion process, the temperature should not be displayed. When the temperature is lower than 120°C, the obtained extrudate is dried with hot air or natural air to make the water content less than 8%, and then pulverized, so that the obtained powder can pass through a 100-mesh sieve.

[0022] 5. Enzymatic hydrolysis and separation and purification

[0023] ①Alkaline protease enzymolysis: use extruded zein as raw material for enzymatic hydrolysis, the substrate concentration is 5%, the enzyme concentration (E/S) is 1.5%, the temperature is 45°C, the pH value is 8.0, and the time is 2 hours. . Then separate the enzymatic hydrolysate.

[0024] ② Papain enzymatic hydrolysis: take alkaline protease hydrolysate as raw material, temperature 45℃, enzyme concentration (E/S) 1%, substrate concentration 5%, pH value 6.5, time 2 hours.

[0025] ③Activated carbon adsorption separation and purification: the product obtained in step ② is subjected to activated carbon adsorption purification at a temperature of 20°C, the mass ratio of activated carbon to the enzymatic hydrolysis solution to be processed is 1:5, and the pH value is 2.0 to maximize the removal of aromatic amino acids and retain branched chains Amino acid components.

[0026] Example 2

[0027] 1. Corn syrup degreasing and odor removal treatment

[0028] Supercritical CO 2 Fluid extraction, extraction pressure 20MPa, temperature 45℃, time 1 hour, CO 2 The flow rate is 20L/hour.

[0029] 2. Extraction of Zein

[0030] Supercritical CO 2The zein powder after fluid extraction is used as a raw material, and the zein solution is extracted for 2 hours under the conditions of an ethanol concentration of 70%, a mass ratio of corn oxen powder and ethanol solution of 1:9, an extraction temperature of 55°C, and a pH value of 8.5. The following is the same as in Example 1.

[0031] 3. Lubricant treatment

[0032] Add an appropriate amount of 25°C drinking water to the zein powder, stir evenly, and leave it for 15 minutes to make the zein powder fully absorb water and ensure that its water content is 20%.

[0033] 4. High temperature and high pressure extrusion processing

[0034] Preheat the twin-screw extruder and set the temperature to 130°C. When the temperature of the die head reaches 130°C, start the extruder for extrusion operation and control the feeding speed of 20kg/hour. During the extrusion process, the temperature should not be displayed. When the temperature is lower than 130°C, the obtained extrudate is dried with hot air or natural air to make the water content less than 8%, and then pulverized, so that the obtained powder can pass through a 100-mesh sieve.

[0035] 5. Enzymatic hydrolysis and separation and purification

[0036] ①Alkaline protease enzymolysis: use extruded zein as raw material for enzymatic hydrolysis, substrate concentration is 7%, enzyme concentration (E/S) is 3.0%, temperature is 50℃, pH value is 9.0, time is 3 hours . Then separate the enzymatic hydrolysate.

[0037] ② Papain enzymatic hydrolysis: take alkaline protease hydrolysate as raw material, temperature 50℃, enzyme concentration (E/S) 3%, substrate concentration 7%, pH value 7.5, time 3 hours.

[0038] ③Activated carbon adsorption separation and purification: The product obtained in step ② is purified by activated carbon adsorption at a temperature of 25°C, the mass ratio of activated carbon to the enzymatic hydrolysate to be processed is 1:7, and the pH value is 3.0 to maximize the removal of aromatic amino acids and retain branched chains Amino acid components.

[0039] Example 3

[0040] 1. Corn yellow powder degreasing and deodorizing treatment

[0041] Supercritical CO 2 Fluid extraction, extraction pressure 30MPa, temperature 45℃, time 1.5 hours, CO 2 The flow rate is 25L/hour.

[0042] 2. Extraction of Zein

[0043] Supercritical CO 2 The zein powder after fluid extraction is used as the raw material, and the zein solution is extracted for 2 hours under the conditions of 80% ethanol concentration, 1:10 mass ratio of corn oxen powder and ethanol solution, extraction temperature of 60°C, and pH value of 9.0. The following is the same as in Example 1.

[0044] 3. Lubricant treatment

[0045] Add an appropriate amount of 25°C drinking water to the zein powder, stir evenly, and leave it for 15 minutes to make the zein powder fully absorb water and ensure that its water content is 25%.

[0046] 4. High temperature and high pressure extrusion processing

[0047] Preheat the twin-screw extruder and set the temperature to 140℃. When the temperature of the die head reaches 140℃, start the extruder for extrusion operation, and control the feeding speed of 25kg/hour. During the extrusion process, the temperature should not be displayed. Below 140°C, the obtained extrudate is dried with hot air or natural air to make the water content less than 8%, and then pulverized, so that the obtained powder can pass through a 100-mesh sieve.

[0048] 5. Enzymatic hydrolysis and separation and purification

[0049] ①Alkaline protease enzymolysis: use extruded zein as raw material for enzymatic hydrolysis, the substrate concentration is 9%, the enzyme concentration (E/S) is 4.5%, the temperature is 55°C, the pH value is 9.0, and the time is 4 hours . Then separate the enzymatic hydrolysate.

[0050] ② Papain enzymatic hydrolysis: take alkaline protease hydrolysate as raw material, temperature 55℃, enzyme concentration (E/S) 5%, substrate concentration 9%, pH value 7.5, time 4 hours.

[0051] ③Activated carbon adsorption, separation and purification: The product obtained in step ② is purified by activated carbon adsorption at a temperature of 30°C, the mass ratio of activated carbon to the enzymatic hydrolysate to be processed is 1:9, and the pH value is 3.0 to maximize the removal of aromatic amino acids and retain branched chains. Amino acid components.