Nisin separating and preparing method
A technology of nisin and nisin, which is applied in the field of separation, peptide extraction and purification, can solve the problems of pollution, low yield, high energy consumption and high cost, and achieve the goal of improving separation efficiency, reducing energy consumption and solving environmental problems Effect
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Embodiment 1
[0038] 1. Yeast liquid pretreatment:
[0039] The nisin fermented liquid produced by the fermentation of Streptococcus nisin is adjusted to pH 2.0-2.6 with hydrochloric acid aqueous solution, and kept at 60-95° C. for 10-20 minutes.
[0040] 2. Foam separation
[0041] The method of solid-liquid separation is used to remove solids such as bacterial fragments in the pretreated fermentation broth, and then foam separation is carried out. Bubble column is used for intermittent separation, the temperature is 45-55°C, the pH is 2.0-2.6, and the superficial gas velocity is 6×10 -6 -6×10 -3 m / s, the demulsification method adopts the mechanical method, and the foam is broken by stirring. The operation time of one cycle is 1.5-2.5 hours, and the demulsification of high concentration nisin is obtained, and the concentration ratio is 16-20. The yield of nisin is 80-90%.
[0042] 3. Salting out
[0043] The soluble Nisin in the broken emulsion is added to ammonium sulfate at a weight...
Embodiment 2
[0047] 1. Yeast liquid pretreatment:
[0048] The nisin fermented liquid produced by the fermentation of Streptococcus nisin is adjusted to pH 2.3-2.8 with hydrochloric acid aqueous solution, and kept at 50-85° C. for 15-25 minutes.
[0049] 2. Foam separation
[0050] Directly use the pretreated fermentation broth for foam separation. Use a bubble column for intermittent separation, the temperature is 30-50 °C, the pH is 2.3-2.8, and the superficial gas velocity is 1×10 -6 -1×10 -2 m / s, the demulsification method adopts the method of adding organic solvents, adding absolute ethanol to the spray flow according to 0.1-5% of the demulsification amount, and the operation time of one cycle is 2-4.0 hours to obtain high-concentration nisin demulsification, and its concentration ratio for 12-18. The yield of nisin was 75-85%.
[0051] 3. Salting out
[0052] Add ammonium sulfate and sodium chloride to the soluble Nisin in the broken emulsion at a weight ratio of 4:1, and it wi...
Embodiment 3
[0056] 1. Yeast liquid pretreatment:
[0057] The nisin fermented liquid produced by the fermentation of Streptococcus nisin is adjusted to pH 2.5-3.0 with hydrochloric acid aqueous solution, and kept at 40-80° C. for 20-30 minutes.
[0058] 2. Foam separation
[0059] The method of solid-liquid separation is used to remove solids such as bacterial fragments in the pretreatment fermentation broth and to separate foam. Use a bubble column for intermittent separation, the temperature is 30-50 °C, the pH is 2.50-3.0, and the superficial gas velocity is 2×10 -6 -2×10 -2 1m / s, the demulsification method adopts the method of adding demulsifier, and the polyether surfactant is added to the spray flow according to 0.01-5% of the demulsification amount, and the operation time of one cycle is 2.5-4.0 hours to obtain the demulsification of high concentration nisin. Its concentration factor is 10-16. The yield of nisin is 85-95%.
[0060] 3. Salting out
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