Preparation of low-molecular weight chitoglycan or chitooligose

A low molecular weight, chitosan technology, applied in the field of chemical biology of renewable resources, can solve the problems of difficult to obtain in large quantities, harsh reaction conditions, modification, etc., to achieve the effect of improving immobilization rate, good mechanical properties, and increasing affinity

Inactive Publication Date: 2006-03-15
WUHAN UNIV
View PDF0 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods for preparing low molecular weight chitosan and chitosan oligosaccharide mainly include chemical method and biological method. The reaction process of chemical method is violent, it is not easy to obtain low molecular weight chitosan and the product structure is modified.
Enzymatic degradation only breaks the β(1-4) glycosidic bonds of chitosan, and generally does not change the residue structure of chitosan molecules, but specific enzymes are expensive and difficult to obtain in l...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: N-succinyl chitosan is made into 0.25g / ml aqueous solution, squeezes into 30ml 0.3g / ml calcium chloride and 70ml dehydrated alcohol to form with syringe (3# needle) dropwise under magnetic stirring In the mixed solution, stirring was continued at room temperature for 6 hours to obtain N-succinyl chitosan hydrogel balls. Weigh 4 g (wet weight) of this hydrogel ball, place it in 12 ml of citric acid-disodium hydrogen phosphate buffer solution (0.05 M, pH 3.0) dissolved with 0.5% glutaraldehyde by volume, and shake at room temperature for 12 hours. The cross-linked N-succinyl chitosan hydrogel balls were collected by filtration, and washed repeatedly with water until the washing solution had no absorption at 245 nm. Dissolve the neutral protease with a ratio of 0.2 to the weight of the immobilized carrier (wet weight) in 0.05M citric acid-disodium hydrogen phosphate buffer solution (pH 3.0), add the cross-linked immobilized carrier to the enzyme solution, and...

Embodiment 2

[0016] Embodiment 2: N-succinyl chitosan is made into 0.30g / ml aqueous solution, squeezes into 40ml 0.15g / ml calcium chloride and 60ml dehydrated alcohol to form with syringe (4# needle) dropwise under magnetic stirring In the mixed solution, stirring was continued at room temperature for 3 hours to obtain N-succinyl chitosan hydrogel balls. Weigh 4 g (wet weight) of this hydrogel ball, place it in 16 ml of citric acid-disodium hydrogen phosphate buffer solution (0.2 M, pH 3.5) with a volume percentage of 0.8% glutaraldehyde, and shake at room temperature for 10 hours. The cross-linked N-succinyl chitosan hydrogel balls were collected by filtration, and washed repeatedly with water until the washing solution had no absorption at 245 nm. Dissolve the neutral protease with a ratio of 0.45 to the weight of the immobilized carrier (wet weight) in 0.2M citric acid-disodium hydrogen phosphate buffer solution (pH 3.5), add the cross-linked immobilized carrier to the enzyme solution, ...

Embodiment 3

[0017] Embodiment 3: N-succinyl chitosan is made into 0.30g / ml aqueous solution, squeezes into 50ml 0.2g / ml calcium chloride and 50ml dehydrated alcohol to form with syringe (5# needle) dropwise under magnetic stirring In the mixed solution, stirring was continued at room temperature for 4 hours to obtain N-succinyl chitosan hydrogel balls. Weigh 4 g (wet weight) of this hydrogel ball, place it in 20 ml of citric acid-disodium hydrogen phosphate buffer solution (0.3 M, pH 4.0) with a volume percentage of 1.0% glutaraldehyde, and shake at room temperature for 8 hours. The cross-linked N-succinyl chitosan hydrogel balls were collected by filtration, and washed repeatedly with 0.3M citric acid-disodium hydrogen phosphate buffer solution (pH 4.0) until the washing solution had no absorption at 245nm. Dissolve the neutral protease with a ratio of 0.6 to the weight of the immobilized carrier (wet weight) in 0.3M citric acid-disodium hydrogen phosphate buffer solution (pH 4.0), add t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

Production of low-molecular weight chitoglycan or chitooligose is carried out by fixing dispase on N-succinchitoglycan aquogel ball from cross-linking method, degrading chitoglycan from fixed enzyme, controlling enzymolysis time, regulating degraded product pH to 8-9 from sodium hydroxide, depositing by alcohol, washing and vacuum drying to obtain low-molecular weight chitoglycan or chitooligose with free amidogen. It achieves much yield, less molecular weight distribution and continuous industrial production. It can be used for medicine and food.

Description

technical field [0001] The invention relates to a preparation method of low molecular weight chitosan or chitosan oligosaccharide, which belongs to the field of chemical biology of renewable resources. Background technique [0002] Low molecular weight chitosan and chitosan oligosaccharide are the degradation products of chitin deacetylation, which have better solubility and biocompatibility than high molecular weight chitosan. Chitosan with different molecular weights has different biological and physiological activities, so the preparation of chitosan with different molecular weights is of great significance for the application of chitosan in food and medicine. At present, the methods for preparing low molecular weight chitosan and chitosan oligosaccharide mainly include chemical method and biological method. The reaction process of chemical method is violent, it is not easy to obtain low molecular weight chitosan and the product structure is modified. Enzymatic degradati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08B37/08C12P19/04
Inventor 杜予民李瑾
Owner WUHAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap