Corn SOD liposome and its preparing method

Abstract

This invention is a corn SOD liposome and its preparation method; it includes corn SOD and liposome film. The feature is that its materials components and weight fraction is that soybean hydrolecithin 6, cholesterol 6, normal hexane 18, chloroform 18, water 10, peridium material is soybean hydrolecithin, the liposome is prepared by rotating film- supersonic wave method. Its preparation technique is simple, and envelope rate and cell compatibility is improved. Physical method is used to protect SOD from degrading by protease, it can be made into oral taking SOD preparation. The enzymology ability of the prepared corn SOD liposome is almost the same to natural SOD. The particle diameter distributing of the SOD liposome is 57mm plus or minus 21mm, the molecular diameter that less than unsaturated lecithin liposome can penetrate cell membrane easier to be absorbed by human being.

Description

technical field [0001] The invention relates to a method for preparing liposomes of enzyme molecules. Background technique [0002] Liposomes are artificially prepared closed capsule structures that are spontaneously formed in water by the hydrophobic interaction between phospholipid molecules, and drugs are encapsulated inside the liposomes. Liposomes are easy to prepare, non-toxic to the body, and non-immunogenic. After entering the body, they are mainly phagocytized by grid cells, so the utilization rate of drugs can be improved. The components of liposome membranes are generally lecithin and cholesterol. Lecithin itself is a component of body cells. It is a physiologically active substance that regulates body functions and can promote metabolism and enhance the body's resistance. The plastids can be made into injections, oral preparations or aerosols, etc. to achieve multiple routes of administration. Therefore, it has attracted much attention as a new type of drug car...

AI Technical Summary

Problems solved by technology

The film dispersion method is to dissolve lecithin, cholesterol and other lipids and fat-soluble drugs in chloroform (or other organic solvents), and then rotate to evaporate to form a film on the inner wall of the bottle, and dissolve water-soluble drugs in phosphate buffered saline. solution, add it to the flask and stir continuously to obtain macroporous liposomes. The encapsulation efficiency of this liposome is generally high, but the permeability is poor