Multilayer phospholipid protein microcapsule for drug slow release wall material and its preparation method

A technology of protein and microcapsules, which is applied in the direction of microcapsules, capsule delivery, non-effective ingredients of polymer compounds, etc., can solve the problems of fragmentation, incomplete microcapsules, and changes in the physical and chemical properties of wall materials, and achieve easy control and preparation The effect of simple device and stable properties

Inactive Publication Date: 2006-10-04
SOUTHEAST UNIV
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  • Claims
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Problems solved by technology

The change of condition parameters is likely to make the obtained microcapsules incomplete, only fragments can be obtained
In addition, since nucleation ofte

Method used

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  • Multilayer phospholipid protein microcapsule for drug slow release wall material and its preparation method
  • Multilayer phospholipid protein microcapsule for drug slow release wall material and its preparation method

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preparation example Construction

[0028] The preparation method of the multilayered phospholipid / protein microcapsule used as drug slow-release wall material is to dissolve the fat-soluble phospholipid in an organic solvent, and then disperse it in an aqueous solution containing protein. The specific steps are as follows:

[0029] 1) Use an organic solvent to dissolve fat-soluble phospholipids into an emulsion; dissolve the protein in water to form an aqueous solution,

[0030] 2) Using a syringe pump to form the above emulsion into spherical droplets, then inject the chloroform spherical droplets containing fat-soluble phospholipids into the aqueous solution containing protein to generate a mixed solution,

[0031] 3) Rotate the above mixed solution on a rotary evaporator to volatilize the organic solvent,

[0032] 4) After the organic solvent is volatilized, the redundant polymer in the mixed solution is removed by centrifugation, and the phospholipid microcapsules with protein adsorption are obtained.

[0...

Embodiment 1

[0034] Embodiment 1: prepare the dipalmitoylphosphatidylcholine (DPPC) / bovine serum albumin multilayer polymer microcapsules with a size of about 5 microns:

[0035] Preparation of the oil phase solution: the phospholipid DPPC was dissolved in the organic solvent chloroform to form the oil phase with a concentration of 0.1 mg / ml.

[0036] Aqueous phase solution preparation: protein human serum was dissolved in tris-hydrochloric acid aqueous solution at a concentration of 0.1 mg / ml.

[0037] Use the electromagnetic pump at a speed of 1.0ml / min to make the oil droplets form droplets at the injection port, and disperse them drop by drop in the aqueous solution, then put the flask on a rotary evaporator to evaporate the chloroform completely, and rinse it with distilled water . Then, multi-layer polymer microcapsules are obtained through layer-by-layer adsorption self-assembly, with a size of about 10 microns.

Embodiment 2

[0038] Example 2: The concentration of the oil phase solution is 0.5 mg / ml, and the concentration of the water phase solution is 1.0 mg / ml. The operation steps are the same as above, and multilayer polymer microcapsules with a size of about 20 microns can be obtained.

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Abstract

The invention relates to a process for preparing self-assembled multilayer microcapsule by utilizing oil-water emulsion, wherein multilayer phosphatide / protein microcapsule can be used as the pharmaceutical slow release wall material, the microcapsule comprises liposoluble phosphatide and protein, the concentration of the liposoluble phosphatide is 0.1-1.0mg/ml, the concentration of protein is 0.1-1.0mg/ml, the volumetric ratio of phosphatide and protein is 1-10:10. The preparing process comprises dissolving the liposoluble phosphatide into organic solvent, the dispersing it into protein-containing aqueous solution.

Description

technical field [0001] The invention relates to a method for preparing self-assembled multilayer microcapsules by using an oil-water emulsion. The multilayer phospholipid / protein microcapsules can be used in the field of drug sustained release, and belong to the technical field of drug sustained release wall materials. Background technique [0002] Layer-by-layer adsorption technology is a method developed in recent years to prepare ordered thin films. It utilizes the electrostatic adsorption properties of organic or inorganic anions and cations to form thin films by alternating molecular deposition of counter ion systems. Decher and Hong constructed nanoscale multilayer polyelectrolyte membranes on planar solid media (quartz plates) in 1991 and 1997, respectively. The essence of this method is to alternately adsorb positively charged macromolecules and negatively charged macromolecules on the solid surface through intermolecular electrostatic interaction, so that the solid...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K47/42
Inventor 顾忠泽葛丽芹
Owner SOUTHEAST UNIV
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