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Treatment of chemical diagnostic reagent unit testing target of breast cancer Her-2 immune tissue

A technology for immunohistochemistry and diagnostic kits, which is used in measurement devices, scientific instruments, biological tests, etc., can solve the problems of expensive reagents and troublesome detection, and achieve the effects of simple operation, accurate diagnosis and small molecular weight.

Inactive Publication Date: 2006-11-01
MAIDI BIOLOGICAL MEDICAL TECH SHENYANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some developing countries have established some effective Her-2 / neu gene and protein detection and evaluation standards to guide and predict the effect of treatment, but the reagents are expensive and the detection is troublesome

Method used

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  • Treatment of chemical diagnostic reagent unit testing target of breast cancer Her-2 immune tissue
  • Treatment of chemical diagnostic reagent unit testing target of breast cancer Her-2 immune tissue

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: The specific operation steps of the present invention for tissue section immunohistochemical staining diagnosis are as follows:

[0023] 1. Synthesis of PAMAM Dendrimer.

[0024]PAMAM Dendrimer is from generation 0 to generation 10, and its molecular diameter is between 10 Ȧ and 130 Ȧ, belonging to nanoscale molecules. Its synthesis adopts the branching method, that is, the molecules grow outward from the core. According to the law of tree branches, the pre-fabricated monomers are combined in a radial order, the branches of the branches are connected to the branches, and the combination is repeated in sequence. With ethylenediamine (EDA) as the initiator nucleus, the first step reaction (a) and methyl acrylate (MA) generate a tetrabasic ester [called 0.5 generation (G)] through Michael addition reaction; the second step reaction (b) The ammonolysis reaction of tetrabasic ester and excess ethylenediamine produces a tetrabasic amide compound (called the fi...

Embodiment 2

[0057] This antibody is also suitable for examination of tumor puncture exfoliated cells. Cancer tissue tumor punctured cell smear staining or cell culture on glass slides; punctured cells were evenly spread on glass slides and immediately fixed in 10% neutral formalin solution for 10 minutes after drying. Cell culture on glass slides can be placed directly in fixative for 10 minutes as cancer cells adhere to the slide. After distilled water is cleaned, directly enter the 8th step among the embodiment one, use 0.3% hydrogen peroxide methanol solution (H 2 o 2 ; 1ml: Methanol; 99ml) to block endogenous enzymes, react for 30 minutes at room temperature and wash with PBS 3 times. The following other steps are the same as in Embodiment 1.

Embodiment 3

[0059] This kit can also be used to detect the expression of Her-2 in other various cancer tissues. Same method

[0060] Embodiment one.

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Abstract

A method for processing detected object of Her-2 immunohistochemistry diagnosis kit for breast cancer includes fixing human breast cancer tissue and carrying out antigenicity recovery treatment then staining it, utilizing biological character of high polymer PAMAM dendron shaped matter containing ammonia functional group to label reaction of antigen and antibody, using color former to make color display on it then observing detection result under microscope.

Description

technical field [0001] The present invention is a divisional application with the patent application date being May 14, 2004, the application number being 200410020546.X, and the invention name being "Her-2 immunohistochemical diagnostic kit for breast cancer". [0002] The invention relates to a processing method for detection objects of a Her-2 immunohistochemistry diagnostic kit suitable for breast cancer. This method of processing the detection object of the kit for immunohistochemical diagnosis of Her-2 in breast cancer by using the biological characteristic labeling antibody of PAMAM dendrites can also be used for the ELESA method and various other cancer tissue diagnoses Detection of Her-2 expression. Background technique [0003] In recent years, the number of breast cancer patients is rising sharply, and the incidence rate ranks first among female tumors. About 50,000 to 90,000 women in my country develop breast cancer every year, and 30,000 to 40,000 women die of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30G01N1/44G01N21/00G01N33/53
Inventor 李文欣
Owner MAIDI BIOLOGICAL MEDICAL TECH SHENYANG
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