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A method for preparing feedstuff addictive from yeast

A feed additive and yeast technology, applied in protein food processing, fungi, food science and other directions, can solve the problems of high cost and complex process, and achieve the effect of low production cost, simple process and great popularization and application value.

Active Publication Date: 2006-11-22
LIFECOME BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above disclosed technologies all belong to the production of yeast by fermentation or the extraction of nucleotide refined products from beer yeast, etc., the process is complicated and the cost is high
There is no report on the simple and low-cost direct production of feed additives containing 4 nucleotide monomers (AMP, GMP, UMP, CMP), protein hydrolyzate and β-glucan from yeast cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Take 1000ml of fermented bread yeast emulsion containing 10% dry weight, quickly heat up to 95°C, inactivate the enzyme, and cool down to room temperature;

[0022] 2. Add sodium hydroxide so that the concentration of sodium hydroxide in the emulsion is 0.25N, and stir for 30-60 minutes above 20°C;

[0023] 3. Determination of yeast cells and RNA content;

[0024] 4. Use hydrochloric acid to adjust the pH to 7.5, and keep it warm at 55°C; according to the measured yeast cell content, add Alcalase 2.4L protease at 0.2% of the weight of the yeast cells contained, stir slowly, and after 4 hours of enzymatic hydrolysis, quickly heat up to 95°C, Inactivate enzyme;

[0025] 5. Then adjust the pH to 5.6 with hydrochloric acid, and keep it warm at 65°C; add 3000u pentaphosphodiesterase per gram of ribonucleic acid according to the measured ribonucleic acid content, stir slowly, and after 3 hours of enzymatic hydrolysis, quickly heat up to 95°C, and extinguish enzyme;

[0...

Embodiment 2

[0028] 1. Precipitate yeast sludge from the brewery, adjust the pH to 8-9, and then wash to obtain wet yeast, and mix it into 1000ml of yeast emulsion containing 12% of yeast cells (dry weight); quickly heat up to 95°C, inactivate the enzyme, to room temperature;

[0029] 2. Add sodium hydroxide so that the concentration of sodium hydroxide in the emulsion is 0.25N, and stir for 30-60 minutes above 20°C;

[0030] 3. Determination of yeast cells and RNA content;

[0031] 4. Adjust the pH to 7.0 with hydrochloric acid and keep it warm at 55°C; according to the measured yeast cell content, add papain at 0.1% of the weight of the yeast cells contained, stir slowly, and after 5 hours of enzymatic hydrolysis, quickly heat up to 95°C to inactivate the enzyme ;

[0032] 5. Adjust the pH to 5.8 with hydrochloric acid and keep warm at 65°C; add 5000u of pentaphosphodiesterase per gram of ribonucleic acid according to the measured ribonucleic acid content, stir at a slow speed, and aft...

Embodiment 3

[0035] 1. Prepare 1000ml of cell suspension containing 10% (dry weight) of yeast by pressing the crushed block of Baidi enzyme, heat up to 95°C quickly, inactivate the enzyme, and cool down to room temperature;

[0036] 2. Add sodium hydroxide so that the concentration of sodium hydroxide in the emulsion is 0.25N, and stir for 30-60 minutes above 20°C;

[0037] 3. Determination of yeast cells and RNA content;

[0038] 4. Use hydrochloric acid to adjust the pH to 7.0, and keep it warm at 55°C; according to the measured yeast cell content, add bromelain at 0.3% of the yeast cell weight, stir slowly, and after 6 hours of enzymolysis, quickly heat up to 95°C to inactivate the enzyme ;

[0039] 5. Then adjust the pH to 5.5 with hydrochloric acid and keep warm at 65°C; add 2500u pentaphosphodiesterase per gram of ribonucleic acid according to the measured ribonucleic acid content, stir at a slow speed, and after 3 hours of enzymatic hydrolysis, quickly heat up to 95°C to extinguish...

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PUM

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Abstract

The feed additive preparing process with yeast emulsion, including fresh yeast emulsion and yeast emulsion compounded with dry yeast powder, is disclosed. The preparation process includes dilute sodium hydroxide solution treatment of yeast emulsion; twice enzymolysis after adding proteinase and pentaphosphodiesterase separately; centrifugally separating the enzymolyzed liquid into supernatant and dreg slurry; and separate concentration and drying of the supernatant and dreg slurry to obtain two feed additive products, including the product I containing four nucleotide monomers, AMP, GMP, CMP and UMP, in the content of 8-24 wt% and protein hydrolysate in the content of 50-60 wt%, and the product II containing beta-glucosan.

Description

technical field [0001] The invention relates to a method for preparing a feed additive containing a mixture of nucleotide monomers and protein hydrolyzate and a feed additive containing beta-glucan from yeast raw materials. Background technique [0002] There are multiple techniques for obtaining nucleotides from yeast thalline that have been disclosed. For example, CN1370837 discloses a method for extracting nucleotides from beer waste yeast; The method; CN13970779 discloses the method for extracting nucleotide from monosodium glutamate waste liquid. The above-mentioned disclosed technologies all belong to fermentation production of yeast or extraction of refined nucleotide products from brewer's yeast, etc., and the process is complicated and the cost is high. There is no report on the direct production of 4 nucleotide monomers (AMP, GMP, UMP, CMP), protein hydrolyzate and β-glucan-containing feed additives from yeast cells with simple and low cost. Contents of the inve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/34C12N1/16
Inventor 贺鹰抟梁超
Owner LIFECOME BIOCHEM