Method for controlling quality of spore oil, powder and preparation of ganoderma lucidum

A quality control method and technology of Ganoderma lucidum spore oil, applied in the field of quality analysis of medicines or health products, can solve the problems of low specificity, few triterpenes, and undetectable, etc., and achieve strong operability, strong specificity, heavy Highly visible effect

Active Publication Date: 2006-12-06
GUANGZHOU HANFANG PHARMA
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Existing reports on controlling the quality of Ganoderma lucidum spores mainly focus on the content determination of water-soluble components of Ganoderma lucidum polysaccharides, while the determination of fat-soluble components mainly uses ultraviolet spectroscopy to determine the content of total triterpenes in Ganoderma lucidum spore oil, but in Ganoderma lucidum spores The content of triterpenes in the powder is very little, and the specificity is not strong when measuring, and the total triterpenoids can also be measured in other vegetable oils by the same method; the single component of the total triterpenes can hardly be detected by high performance liquid chromatography. Composition, used to evaluate the quality of spore powder and spore oil has certain limitations
There are still no literature reports on evaluating the quality of Ganoderma lucidum spore powder, spore oil and their preparations with the content of ergosterol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, the research of the method for measuring ergosterol content in Ganoderma lucidum spore oil by high performance liquid chromatography

[0024] (1) Instruments and samples

[0025] Instruments: Agilent 1100 high performance liquid chromatography, DAD detector, quaternary gradient pump, G2170AA data processing software system (USA). Methanol was chromatographically pure, water was ultrapure water, and other reagents were analytically pure.

[0026] Samples: The reference substance ergosterol was provided by sigma; Ganoderma lucidum spore oil was provided by Guangzhou Hanfang Modern Chinese Medicine Research and Development Co., Ltd.

[0027]Preparation of the test solution: Accurately weigh 1g of Ganoderma lucidum spore oil, add 10ml of petroleum ether to dissolve it, add it on a silica gel column (100-200 mesh, 10g, inner diameter 15mm), use petroleum ether: ethyl acetate as 90: 10 solution 100ml elution, discard the eluent, and then use petroleum ether: e...

Embodiment 2

[0053] Embodiment 2, determination of ergosterol content in Ganoderma lucidum spore oil

[0054] According to the method for content determination of the above-mentioned Example 1, the content of ergosterol in 10 samples of Ganoderma lucidum spore oil was determined, and the results are shown in Table 6.

[0055] sample

[0056] The content of ergosterol in every 1g of Ganoderma lucidum spore oil is measured to be between 0.423-1.920mg.

Embodiment 3

[0057] The content determination of ergosterol in embodiment 3, Ganoderma lucidum spore oil emulsion

[0058] Determination by high performance liquid chromatography:

[0059] Chromatographic conditions and wavelength selection Octadecyl bonded silica gel is used as filler; methanol is used as mobile phase; detection wavelength is 280nm. The number of theoretical plates is calculated according to the ergosterol peak and should not be less than 2000;

[0060] Preparation of reference substance solution: take an appropriate amount of ergosterol reference substance, add methanol to make a solution containing 0.08mg per 1ml, and obtain the reference substance solution;

[0061] Preparation of test solution: Accurately weigh 10 g of ganoderma lucidum spore oil emulsion, add 2 g of anhydrous sodium sulfate, heat on a water bath to rupture the emulsion, transfer to a separatory funnel, extract 3 times with ether, 20 ml each time, combine Diethyl ether solution, evaporated to drynes...

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Abstract

An oil or powder of ganodema spore is disclosed. Its quality control method features that the efficient liquid-phase chromatographic method and the thin-layer scanning chromatography method are used to measure the content of ergosterol in the product.

Description

technical field [0001] The invention relates to a method for analyzing the quality of medicines or health care products, in particular to a method for measuring the content of ergosterol in ganoderma lucidum spore oil, ganoderma lucidum spore powder and preparations thereof. Background technique [0002] Ganoderma lucidum is the general name of Ganoderma lucidium (Leyss.ex Fr.) Karst.] and Zizhi [Ganodermajaponicum (Fr.) Lloyd.] of the genus Ganoderma (Ganoderma) of the family Polyproraceae. Efficacy, known as top grade by "The Classic". Ganoderma lucidium spores are extremely small spores ejected from the cap during the mature growth stage of Ganoderma lucidum. They are the germ cells of Ganoderma lucidum and contain all the genetically active substances of Ganoderma lucidum. Their medicinal value is also increasingly valued. The pharmacological effects of Ganoderma lucidum spores mainly include anti-tumor, immune regulation, regulation of blood lipids, and regulation and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/074G01N30/02G01N30/90G01N33/15
Inventor 李吉来宁德山刘菊妍陈路林庞胜凤吴德玄
Owner GUANGZHOU HANFANG PHARMA
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