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Chemical illumination immunity analysis instrument

A chemiluminescence immunoassay and analyzer technology, which is applied in the field of quantitative analysis in medical immunoassays, can solve problems such as poor repeatability and accuracy, unstable enzyme labels, and short shelf life, and achieve a wide linear range of testing and instrument structure Simple, highly sensitive effects

Inactive Publication Date: 2007-01-10
上海复生生物工程研究所有限公司
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Problems solved by technology

The disadvantages are: it can only be applied in large hospitals, and it is difficult to spread to grassroots medical institutions; the radioactive isotope r-rays cause radiation damage to the human body; the detection waste causes radioactive pollution to the environment; the shelf life is short, only one month
However, the sensitivity is poor, the repeatability and accuracy are not good, and it is mainly used for qualitative analysis, which is difficult for quantitative analysis; most of the substrates of enzyme label technology are toxic or carcinogens, and cannot be stored, and must be freshly prepared when used; enzyme label itself Also unstable, susceptible to changes in temperature and pH
Therefore, the current problems in clinical immunoassays are: RIA with high sensitivity, accuracy and repeatability cannot be popularized, and EIA, which is easy to popularize and easy to operate, cannot meet clinical requirements
The vast majority of demand units are unable to afford the expensive instrument prices, and can only look at CLIA's high technology.
[0008] At present, semi-automatic chemiluminescence immunoassay instruments that avoid complex and expensive sample integrated processing systems and integrate machinery, control, software, and low-light detection technology are still blank in the market.

Method used

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Embodiment Construction

[0019] figure 1 , figure 2 Respectively be the working principle figure of the present invention and main frame outward appearance figure, by figure 1 It can be seen that the chemiluminescence analyzer provided by the present invention includes a software system (including a host computer and a lower computer), a control system (including a stepping motor drive and an optical probe drive), a mechanical transmission system, and a low-light detection system.

[0020] The software system adopts the driver chip MAX232 conforming to the RS-232 standard. In the serial communication mode, the upper computer transmits control instructions and related parameter values ​​to the lower computer, and the lower computer executes the instructions of the upper computer to control the movement of the stepping motor. Control the photon counter to collect data, and transmit the collected data to the host computer. The upper computer uses Visual Basic high-level language to write software to r...

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Abstract

The present invention discloses chemoluminescence immunity analyser. It includes machine driven system, glimmer light detection system, control system and software system; wherein said machine driven system consisting of one tray, two repeating motors, six threshold circuits and bidirectional guideway, said glimmer light detection system consisting of quantorecorder, impulse counter, high voltage supply and probe, signal from control system positioned through repeating motor driving controlling mechanical system and through optic probe driving controlling glimmer light detection system, said control system being chip 89 C52 constructed microprocessor and making duplex communication with software system through RS 232 interface circuit, said software system consisting of upper computer and lower computer program, lower computer according to executing relevant action according to upper computer and processing information from glimmer light detection system. Compared with current technology the present invention has high sensitivity and wide testing linear range.

Description

Technical field: [0001] The invention relates to a high-sensitivity optical detection device, which is especially suitable for quantitative analysis in medical immunological detection. Background technique: [0002] Currently, there are two main types of techniques used in clinical immunoassays: radioimmunoassay (RIA) and enzyme-linked immunoassay (EIA). Advantages of RIA: high sensitivity, good accuracy and repeatability, and can be used for ultra-trace quantitative analysis. The disadvantages are: it can only be applied in large hospitals, and it is difficult to spread to grassroots medical institutions; the radioactive isotope r-rays cause radiation damage to the human body; the detection waste causes radioactive pollution to the environment; the shelf life is short, only one month. EIA is easy to operate and easy to popularize. However, the sensitivity is poor, the repeatability and accuracy are not good, and it is mainly used for qualitative analysis, which is difficu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N21/76G01N35/00
Inventor 温俊娥杨波
Owner 上海复生生物工程研究所有限公司
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