Method of transforming enzyme to prepare L-ornithine

Abstract

the invention discloses a preparing method of L-ornithine, which comprises the following steps: preparing inclined-plane seed; selecting culture medium; optimizing converting condition; extracting product. the invention improves converting rate of arginine by 99.5%, which provides basic raw material for medical industry.

Description

technical field [0001] The invention relates to a preparation method of L-ornithine. Background technique [0002] At present, there are no reports on the production of L-ornithine by enzymatic conversion at home and abroad. The preparation methods of L-ornithine mainly include chemical method and fermentation method, wherein chemical method is to use arginine as raw material, obtain L-ornithine through alkaline hydrolysis or utilize arginase hydrolysis; and fermentation method is to use Microbial fermentation produces L-ornithine. Microorganisms commonly used in the production of L-ornithine include: Corynebacterium glutamicum, Brevibacterium lactic acid fermenterum, Escherichia coli, Corynebacterium hydrocarbonolyticus, Streptomyces albicans, etc. Due to the harsh conditions of producing L-ornithine by chemical method, a large amount of organic solvents are used, which brings serious pollution to the environment; while the production of L-ornithine by fermentation method ...

AI Technical Summary

Problems solved by technology

Due to the harsh conditions of producing L-ornithine by chemical method, a large amount of organic solvents are used, which brings serious pollution to the environment; while the production of L-ornithine by fermentation method has low yield (generally only 10g / L), long cycle, and many impurities in the product. Separation is difficult; the yield of L-ornithine prepared by the above method is not high, so the cost of L-ornithine produced is very high, which brings great difficulties to practical application