Method of transforming enzyme to prepare L-ornithine

A technology for ornithine and enzyme conversion, applied in the field of L-ornithine preparation, can solve the problems of high cost, long cycle, and difficult separation of L-ornithine, and achieve great industrial significance, safe production operation, and high production efficiency. The effect of mild conditions

Inactive Publication Date: 2007-02-07
山东泓达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the harsh conditions of producing L-ornithine by chemical method, a large amount of organic solvents are used, which brings serious pollution to the environment; while the production of L-ornithine by fermentation method has low yield (generally only 10g / L), long cycle, and many impurities in the product. Separation is difficult; the yield of L-ornithine prepared by the above method is not high, so the cost of L-ornithine produced is very high, which brings great difficulties to practical application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Bacteria slant culture

[0044] Inoculate the slant culture medium of Enterococcus faecalis CGMCC NO.1786 into the test tube to make the slant strain;

[0045] Wherein the bacterial strain slant culture medium is made up of following components by weight:

[0046] 5 parts of glucose, 20 parts of urea, 3.5 parts of corn steep liquor, 1.0 parts of magnesium sulfate, 1.0 parts of dipotassium hydrogen phosphate, 5.0 parts of sodium chloride, 2.0 parts of calcium carbonate, 0.02 parts of iron sulfate, 0.02 parts of manganese sulfate, 15 parts of agar and 1000 parts of water;

[0047] The technological conditions for preparing slant strains are pH value 7.5, temperature 30° C., and culture time 24 hours.

[0048] (2) Inoculation fermentation

[0049] Expand the cultivation of the slant strain as the seed solution;

[0050] Put the seed liquid into the fermenter according to the inoculation amount of 10% to ferment;

[0051] Wherein the seed liquid and the fermented l...

Embodiment 2

[0063] (1) Bacteria slant culture

[0064] Inoculate the slant culture medium of Enterococcus faecalis CGMCC NO.1786 into the test tube to make the slant strain;

[0065] Wherein the bacterial strain slant culture medium is made up of following components by weight:

[0066] 8 parts of glucose, 15 parts of urea, 3.0 parts of corn steep liquor, 1.0 parts of magnesium sulfate, 1.0 parts of dipotassium hydrogen phosphate, 5.0 parts of sodium chloride, 2.0 parts of calcium carbonate, 0.02 parts of iron sulfate, 0.02 parts of manganese sulfate, 15 parts of agar and 1000 parts of water;

[0067] The technological conditions for preparing slant strains are pH value 8.0, temperature 40° C., and incubation time 36 hours.

[0068] (2) Inoculation fermentation

[0069] Expand the cultivation of the slant strain as the seed solution;

[0070] Put the seed liquid into the fermenter according to the inoculation amount of 10% to ferment;

[0071] Wherein the seed liquid and the fermente...

Embodiment 3

[0083] (1) Bacteria slant culture

[0084] Inoculate the slant culture medium of Enterococcus faecalis CGMCC NO.1786 into the test tube to make the slant strain;

[0085] Wherein the bacterial strain slant culture medium is made up of following components by weight:

[0086] 10 parts of glucose, 10 parts of urea, 2.5 parts of corn steep liquor, 1.0 parts of magnesium sulfate, 1.0 parts of dipotassium hydrogen phosphate, 5.0 parts of sodium chloride, 2.0 parts of calcium carbonate, 0.02 parts of iron sulfate, 0.02 parts of manganese sulfate, 15 parts of agar and 1000 parts of water;

[0087] The technological conditions for preparing the slant strain are pH value 7.0, temperature 30° C., and culture time 48 hours.

[0088] (2) Inoculation fermentation

[0089] Expand the cultivation of the slant strain as the seed solution;

[0090] Insert the seed liquid into the fermenter according to the inoculation amount of 15% to ferment;

[0091] Wherein the seed liquid and the ferm...

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Abstract

the invention discloses a preparing method of L-ornithine, which comprises the following steps: preparing inclined-plane seed; selecting culture medium; optimizing converting condition; extracting product. the invention improves converting rate of arginine by 99.5%, which provides basic raw material for medical industry.

Description

technical field [0001] The invention relates to a preparation method of L-ornithine. Background technique [0002] At present, there are no reports on the production of L-ornithine by enzymatic conversion at home and abroad. The preparation methods of L-ornithine mainly include chemical method and fermentation method, wherein chemical method is to use arginine as raw material, obtain L-ornithine through alkaline hydrolysis or utilize arginase hydrolysis; and fermentation method is to use Microbial fermentation produces L-ornithine. Microorganisms commonly used in the production of L-ornithine include: Corynebacterium glutamicum, Brevibacterium lactic acid fermenterum, Escherichia coli, Corynebacterium hydrocarbonolyticus, Streptomyces albicans, etc. Due to the harsh conditions of producing L-ornithine by chemical method, a large amount of organic solvents are used, which brings serious pollution to the environment; while the production of L-ornithine by fermentation method ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/10C12R1/01C12N1/20
Inventor 张鹏张淑荣刘春巧杨育红
Owner 山东泓达生物科技有限公司
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