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Method for producing L-ornithine hydrochloride through immobilized enzyme process

A technology of ornithine hydrochloride and immobilized enzymes, which is applied to the direction of immobilization on/in organic carriers, fermentation, etc., can solve the problems of unfavorable product separation and purification, reduced product yield, increased production cost, etc., to achieve The effect of less impurities, low production cost and safe production operation

Inactive Publication Date: 2010-10-06
湖南天成生化科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The above-mentioned L-ornithinase production process uses free cells to transform the substrate L-arginine, so the transformation solution will contain a small amount of bacterial protein and other impurities, which is not conducive to the separation and purification of the product; and it must be removed after transformation. Bacteria, reducing product yield, resulting in increased production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for producing L-ornithine hydrochloride by enzymatic conversion, characterized in that the method comprises the steps of:

[0033] 1. Preparation method of immobilized enzyme

[0034] (1) Raw material consumption:

[0035] Triethanolamine anionic styrene resin 50g

[0036] Arginase 400mg

[0037] Saturated saline subject to the ability to submerge the immobilized enzyme

[0038] (2) Operation process:

[0039] Soak 50 g of triethanolamine anionic styrene resin in 2M potassium carbonate solution for 12 hours, and filter. After filtering to dryness, add 100ml 2M potassium carbonate solution, add 10ml hydrogen bromide (2g / ml) under ice bath, react for 15 minutes under ice bath, wash with cold 0.1M sodium bicarbonate until pH = 8.5, add arginase 100ml (about 400mg) of the solution was reacted at 4°C for 24h. Wash with 0.1M sodium bicarbonate, 1M sodium chloride, and deionized water three times in sequence, 200ml each time.

[0040] Soak the immobilized enzym...

Embodiment 2

[0055] (1) Preparation of immobilized enzyme

[0056]Take 40g of triethanolamine anionic styrene resin, soak it with a potassium carbonate solution with a molar concentration of 1M for 8h, filter it, and after drying, add 10ml of a potassium carbonate solution with a molar concentration of 1M, and 5ml of a hydrogen bromide solution with a mass concentration of 1g / ml React in an ice bath for 5 minutes, adjust the pH value of the solution to 7 with a cold sodium bicarbonate solution with a molar concentration of 0.05M, then add 50ml of arginase solution with a mass concentration of 1g / l, react at 1°C for 12h, and then Wash twice with 100ml of sodium bicarbonate solution with a molar concentration of 0.05M, 100ml of 0.5M sodium chloride solution, and 100ml of deionized water, and finally soak in saturated saline for 0.5h, wash to prepare the immobilized enzyme, and store in in water;

[0057] (2) Optimization of transformation conditions

[0058] Mix the immobilized enzyme prep...

Embodiment 3

[0062] (1) Preparation of immobilized enzyme

[0063] Take 50g of triethanolamine anionic styrene resin, soak it with molar concentration as 2M potassium carbonate solution for 12h and filter it, after filtering dry, add 100ml of potassium carbonate solution with molar concentration of 2M, 10ml of hydrogen bromide solution with a mass concentration of 2g / ml in React in an ice bath for 15 minutes, adjust the pH value of the solution to 8.5 with a cold sodium bicarbonate solution with a molar concentration of 0.1M, then add 100ml of arginase solution with a mass concentration of 4g / l, react at 4°C for 24 hours, and then use 200ml of sodium bicarbonate solution with a molar concentration of 0.1M, 200ml of a sodium chloride solution with a molar concentration of 1M, and 200ml of deionized water were washed three times, and finally soaked in saturated saline for 1 hour, washed to obtain the immobilized enzyme, and stored in in water;

[0064] (2) Optimization of transformation con...

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PUM

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Abstract

The invention relates to a preparation method for L-ornithine hydrochloride. The L-ornithine hydrochloride is produced through converting arginase. The method comprises the following technological steps: (1) preparing immobilized enzyme; (2) optimizing conversion conditions; and (3) extracting products and refining. Compared with the prior art, the invention has the advantages that the production cost is low, the production conditions are mild, the impurities in the conversion system are less, the technological steps are simple, the production and the operation are safe, the purity is high, one liter of solution contains 100-130g of L-ornithine hydrochloride, the yield is high, the conversion rate of arginine reaches more than 95 percent and the like.

Description

technical field [0001] The invention relates to a method for producing L-ornithine hydrochloride, in particular to a method for producing L-ornithine hydrochloride by an immobilized enzyme method. Background technique [0002] L-ornithine hydrochloride is an important basic raw material of the pharmaceutical industry, an important metabolite in cells, and plays an important role in regulating the elimination of liquid ammonia in organisms. [0003] At present, there are different reports on the production of L-ornithine hydrochloride by enzymatic conversion at home and abroad. There are three main methods for preparing L-ornithine hydrochloride: chemical method, fermentation method and enzymatic method. [0004] 1 chemical method [0005] At present, the most commonly used production method in industry is to prepare L-ornithine by chemical hydrolysis of L-arginine. In 1966, Shell Int. Rearsch used acrylic acid and sodium cyanide as the main raw materials to chemically synt...

Claims

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Application Information

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IPC IPC(8): C12P13/10C12N11/02
Inventor 苏建勇郑南华张建宇
Owner 湖南天成生化科技有限公司
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