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Lysozyme-chitosan films

A technology of chitosan and lysozyme, which can be used in food preservation, cheese substitutes, fruit and vegetable preservation, etc., and can solve the problems of limited application of antibacterial efficacy

Inactive Publication Date: 2007-05-30
THE STATE OF OREGON ACTING BY & THROUGH THE OREGON STATE BOARD OF HIGHER EDUCATION ON BEHALF OF OREGON STATE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its limited antibacterial efficacy against Gram-negative bacteria limits its application in the food industry

Method used

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  • Lysozyme-chitosan films
  • Lysozyme-chitosan films
  • Lysozyme-chitosan films

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Material

[0048] Shrimp chitosan from Vanson Inc. (Redmond, WA) was used without further purification. Shrimp chitosan was characterized by a viscosity of 11 cps in 1% w / w aqueous acetic acid at 25°C and a deacetylation of 89.9%. Egg white lysozyme was obtained from Eiprodukte GmbH und Co. (Germany). USP (United States Pharmacopeia) grade glycerol was obtained from EM Science (Darmstadt, Germany). Reagent grade glacial acetic acid was obtained from J.T. Baker (Phillipsburg, NJ).

[0049] Gram-positive Streptococcus faecalis ATCC 14508 and Gram-negative Escherichia coli type B were used as test organisms. Freeze-dried Micrococcus lysodeikticus obtained from Sigma Chem. Co. (St. Louis, MO) was used for the measurement of lysozyme release profile. Brain heat infusion (BHI), MRS broth and agar were purchased from Difcoof Becton, Dickinson and Company (Sparks, MD).

[0050] Preparation of film-forming solution

[0051] A film-forming solution (FFS) was prepared by mix...

Embodiment 2

[0104] Selective FFS can be achieved by dissolving 2 wt% chitosan in 1 wt% acetic acid solution supplemented with 50 wt% dodecanoic acid, 25 wt% glycerol and 100 wt% lysozyme (w / w chitosan) at 65°C to prepare. Membranes were then prepared as described in Example 1. Addition of fatty acids (ie, dodecanoic acid) to the film-forming solution improved the water resistance of the film without significantly affecting its antimicrobial activity.

Embodiment 3

[0106] A coating solution was prepared by mixing a chitosan solution with a lysozyme solution. A chitosan solution was prepared by dissolving 3% chitosan in a 1% acetic acid solution supplemented with 25% glycerol (w / w chitosan). A lysozyme solution was prepared by dissolving 10% lysozyme in distilled water supplemented with 25% glycerol (w / w lysozyme). The lysozyme solution was mixed into the chitosan solution to a concentration of 60% (percent dry weight of lysozyme per dry weight of chitosan). The resulting mixture was homogenized using a homogenizer (PT 10-35, Kinematica, Switzerland) at 3,000 rpm for 60 seconds. Listeria monocytogenes ATCC 15313 and Lactobacillus plantarum were used as test microorganisms to evaluate the antimicrobial efficacy of coating treatments on food surfaces. Listeria monocytogenes and Lactobacillus plantarum were cultured overnight at 37°C in brain heart infusion (BHI) and MRS broth, respectively.

[0107] Commercially produced beef franks (Bun...

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PUM

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Abstract

One aspect of the disclosure herein contemplates a composite film comprising lysozyme incorporated within a chitosan polymer matrix. In another aspect, there is disclosed a film that includes chitosan, and about 10 to about 200 weight percent lysozyme, based on the weight of the chitosan. Also disclosed is a method for making a film that includes dissolving or dispersing chitosan and lysozyme in an aqueous medium resulting in a film-forming solution or dispersion; applying the film-forming solution or dispersion to a substrate surface; and converting the film-forming solution or dispersion into a film. In a particularly useful application, the film is an antimicrobial protectant for a food article.

Description

[0001] Cross References to Applications [0002] This application claims priority to US Provisional Patent Application No. 60 / 554,623, filed March 18, 2004, which is incorporated herein by reference. technical field [0003] The present invention discloses an antimicrobial film especially for protecting food. Background technique [0004] In solid or semi-solid foods, microbial growth occurs primarily on surfaces. Heat treatment and chemical preservatives are the most widely used methods for maintaining microbiological safety and food quality. Wrap films with enhanced microbial resistance have great potential for ensuring the microbiological safety of food surfaces through the slow release of antimicrobial substances from the supporting film structure to the food surface. In addition, consumer concerns about synthetic chemical food preservatives have led to broader interest in alternative, more "consumer friendly" edible protective films. [0005] Lysozyme is a well-studi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/056A23L1/0562A23L3/3571A23C19/06A23C19/16A23L29/275A23L29/281
CPCA23C19/063A23C19/163A23B4/10A23B7/154A23L3/3571A23L3/3463A23B4/22A23B7/16A23B7/155A23B4/20
Inventor 赵艳云朴首一马克·A·达埃舍尔
Owner THE STATE OF OREGON ACTING BY & THROUGH THE OREGON STATE BOARD OF HIGHER EDUCATION ON BEHALF OF OREGON STATE UNIV
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