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Use of plant extract catechin in preparation of antivirus medicine

A technology for catechins and drugs, applied in the directions of antiviral agents, drug combinations, pharmaceutical formulations, etc., can solve the problems of catechin antiviral, catechin respiratory virus and in vivo antiviral, etc.

Inactive Publication Date: 2007-07-11
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported in foreign literature that EGCG can exert anti-HIV effect by inhibiting the activity of HIV-1 reverse transcriptase in vitro, and can affect the infectivity of influenza virus in vitro by inhibiting the acidification of intracellular endosomes or lysosomes. There are no reports of catechins against other respiratory viruses in vitro and anti-viruses in vivo, and there is no domestic research report on catechins against viruses

Method used

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  • Use of plant extract catechin in preparation of antivirus medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019]Embodiment 1: The catechin in the present invention is to be raw material with green tea extract (the green tea market can be purchased), through organic solvent extraction repeatedly, removes caffeine and other impurity, then separates and obtains by HPLC (see catechin rich Research on the new purification process of Jihe EGCG monomer, Zhang Xinghai, Tea, 2002, 28(3): 136-137), and confirmed by modern analysis methods such as ultraviolet, infrared and mass spectrometry. The catechin monomer extracted in the present invention is off-white powder, wherein the EGCG molecular weight is 458.4, and the ECG molecular weight is 442. Catechin is easily soluble in water and DMSO. It is prepared with sterile double-distilled water to a storage concentration of 20 mg / ml and stored at -20°C in the dark. It has stable properties and curative effect in a short period of time. The toxic effect of catechin on HEp-2 cells and MDCK cells was manifested as increased cell refraction, cell r...

Embodiment 2

[0021] Example 2: Toxic effects of catechins on HEp-2 cells and MDCK cells

[0022] On a 96-well plate, add 0.1ml, 2×10 5 concentration of HEp-2 and MDCK cells, 24 hours after the cells grow into a monolayer, add different concentrations of drug-containing maintenance solution to continue culturing. Cell changes were observed under an inverted microscope every day, and after 72 hours, cell viability was detected by MTT method. Four wells were replicated for each drug concentration. At the same time, a normal cell control was set. Each experiment was repeated 3 times.

[0023] Cell survival rate (%)=(average A value of experimental group / average A value of cell control group)×100%. Statistical software SPSS11.5 was used to analyze the data and calculate the half cytotoxic concentration of the drug (CC 50 ).

[0024] The toxic effects of the drug on the two types of cells were manifested as increased refraction, rounding, fragmentation, shedding, and a decrease in light ab...

Embodiment 3

[0028] Example 3: Pharmacodynamic study of catechin against avian influenza virus, respiratory syncytial virus, Coxsackie virus and adenovirus in vitro

[0029] The inhibitory effects of catechin on avian influenza virus H5N1, RSV Long strain, CVB3 and AdV-7 were tested in the following three ways. Group I (preventive effect of drugs on viral infection): Pre-treat monolayer cells with different concentrations of drugs for 24 hours, wash twice with PBS and wash with 100 TCID 50 Attack with 0.1ml per well of 0.1mL virus, absorb for 1h, discard the virus solution, wash twice with PBS, add cell maintenance solution for culture, observe the changes of cells under an inverted microscope every day; wait until the CPE of the virus control well reaches more than 80% and the cells are in control When normal, the cell viability was detected by the MTT method, and 4 wells were repeated for each drug concentration, and a normal cell control and a virus control were set at the same time. T...

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Abstract

The invention relates to method for using plant extractive catechin to prepare drug, wherein it uses green tea extractive as material; extracts in organic solvent; removes caffeine; uses HPLC to separate the catechin, as epigallocatechin, etc. The inventive drug can be used to treat virus infection of respiratory tract.

Description

technical field [0001] The invention relates to the field of pharmacy, in particular to the application of plant extract catechin in the preparation, treatment or prevention of several common respiratory virus infections such as avian influenza virus, respiratory syncytial virus, coxsackie virus and adenovirus. Background of the invention [0002] Acute respiratory infection is the most common disease in humans, and more than 80% of it is caused by viruses. It is the main cause of morbidity and death in the elderly and children worldwide. There are many types of viruses that cause respiratory tract infections, among which the common ones are influenza virus (Flu), respiratory syncytial virus (RSV), rhinovirus (HRV), and adenovirus (AdV). In recent years, avian influenza viruses have broken through the species barrier to infect humans. Due to the mutation of the virus, the incidence of respiratory virus infection continues unabated, and there is a pandemic. It has become an ...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P31/14A61P31/16A61P11/00
Inventor 杨占秋石丽桥
Owner WUHAN UNIV
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