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Preparation process of total phenolic acids of water cress and pharmaceutical formulation using the ingredient as bulk drug and use thereof for treating liver disease

A technology for pharmaceutical preparations and APIs, which is applied to medical preparations containing active ingredients, drug combinations, plant raw materials, etc., can solve the problems of high cost, large loss of active ingredients, and low yield of total phenolic acids.

Active Publication Date: 2007-07-11
黄正明 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Commonly used extraction and separation processes are: alcohol extraction-water precipitation-ethyl acetate extraction method or acid-base method to obtain total phenolic acids. These methods either cause a large loss of active ingredients or destroy the components, resulting in low yields of total phenolic acids and high costs.

Method used

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  • Preparation process of total phenolic acids of water cress and pharmaceutical formulation using the ingredient as bulk drug and use thereof for treating liver disease

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0011] 1. Anti-tumor experiments in vivo and in vitro

[0012] 1.1 The antitumor effect of cress total phenolic acid (FS)

[0013] Table 1 Anti-tumor experiment of cress total phenolic acid

[0014] sample

[0015] Note: The results showed that the total phenolic acid of cress had inhibitory effects on the above three cancer cells, and it was dose-dependent.

[0016] 1.2 Anti-liver cancer effect of cress total phenolic acid (FS)

[0017] (1) The inhibitory effect of FS 10 and 20 mg / ml on mouse liver cancer 22 (H22): the inhibition rates were 46.8 and 49.1% respectively (P<0.01=

[0018] (2) FS 10 and 20mg / ml are effective against mouse sarcoma 180 (S 180 ) inhibition: the inhibition rates were 44.6, 74.8% (P<0.01)

[0019] 2. Anti-hepatitis B virus (HBV) effect of cress total phenolic acid (FS)

[0020] Table 2 The inhibitory effect of total phenolic acids of cress on HBV-DNA

[0021] experiment

[0022]...

experiment example 2

[0023] Experimental example 2: preparation of cress total phenolic acid raw material

[0024] Grind the dried cress whole herb into a coarse powder, add 10 times the amount of 70% ethanol to heat and reflux for 2 hours, a total of 3 times, combine the extracts, let cool, filter, and then store the extracts at a temperature lower than 60°C Concentrate under reduced pressure to an extract with a specific gravity of 1.15-1.30 (70°C); add hot water 6 times the amount of the crude drug, dissolve in stages, let stand for 24 hours, filter, add the filtrate to 5 times the amount of the crude drug, and have processed On the polyamide column, rinse with water until the effluent is light yellow, elute with 70% alcohol, collect the ethanol eluate until the eluate bears yellow color, concentrate under reduced pressure at a temperature lower than 60°C, and dry in vacuo to obtain Cress total phenolic acid extract.

experiment example 3

[0026] Preparation of capsules

[0027] Cress total phenolic acid raw material 100g, medicinal starch 100g

[0028] Pass the total phenolic acid of cress and medicinal starch through a 100-mesh sieve, mix evenly, and granulate with an appropriate amount of ethanol. Dry at 60°C, granulate, and pack into capsules.

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Abstract

The invention relates to a method for producing Oenanthe stolonifera phenolic acid, wherein it comprises hyperin, isoquercetin, chlorogenic acid, etc, wherein it comprises that: taking Oenanthe stolonifera; adding alcohol to extract; recycling alcohol to obtain alcohol paste; dissolving via boiling water, filtering and taking upper clear liquid as polyamide post; washing with water, washing with alcohol; collecting liquid, recycling alcohol, vacuum drying to obtain paste; using chromatics to test the content of phenolic acid; when using said paste as effective component, the content of phenolic acid can reach 50%, to reach 5-traditional medicine standard. The invention also provides relative application in the preparation of cancer resistant drug.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of effective parts of plant medicines, can make the total phenolic acid content of the effective parts of watercress greater than 50%, and reach the national standard of five types of traditional Chinese medicine raw materials; use. Background technique [0002] Commonly used extraction and separation processes are: alcohol extraction-water precipitation-ethyl acetate extraction method or acid-base method to obtain total phenolic acids. These methods either cause a large loss of active ingredients or destroy the components, resulting in low yields of total phenolic acids and high costs. Therefore, it is necessary to study a new simple and efficient extraction and separation method, which is suitable for industrial production. Contents of the invention [0003] The purpose of the present invention is to disclose the method for preparing total phenolic acid of cress; another p...

Claims

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Application Information

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IPC IPC(8): A61K36/23A61P1/16A61P35/00A61P31/14A61K127/00
Inventor 黄正明杨新波曹文斌陈红艳杨坤
Owner 黄正明