Purified hepatitis C virus envelope proteins for diagnostic and therapeutic use

a technology of hepatitis c virus and envelope proteins, which is applied in the direction of viruses, peptides/protein ingredients, fungi, etc., can solve the problems of much more expensive manufacturing of hbsag from mammalian cells compared with yeast-derived hepatitis b vaccines
US20030036110A1Inactive Publication Date: 2003-02-20INNOGENETICS NV
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
INNOGENETICS NV
Publication Date
2003-02-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to a method for purifying recombinant HCV single or specific oligomeric envelope proteins selected from the group consisting of E1 and / or E1 / E2 characterized in that upon lysing the transformed host cells to isolate the recombinantly expressed protein a disulphide bond cleavage or reduction step is carried out with a disulphide bond cleavage agent. The present invention also relates to a composition isolated by such a method. The present invention also relates to the diagnostic and therapeutic application of these compositions. Furthermore, the invention relates to the use of HCV E1 protein and peptides for prognosing and monitoring the clinical effectiveness and / or clinical outcome of HCV treatment.
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Description

[0001] The present invention relates to the general fields of recombinant protein expression, purification of recombinant proteins, synthetic peptides, diagnosis of HCV infection, prophylactic treatment against HCV infection and to the prognosis / monitoring of the clinical efficiency of treatment of an individual with chronic hepatitis, or the prognosis / monitoring of natural disease.

[0002] More particularly, the present invention relates to purification methods for hepatitis C virus envelope proteins, the use in diagnosis, prophylaxis or therapy of HCV envelope proteins purified according to the methods described in the present invention, the use of single or specific oligomeric E1 and / or E2 and / or E1 / E2 envelope proteins in assays for monitoring disease, and / or diagnosis of disease, and / or treatment of disease. The invention also relates to epitopes of the E1 and / or E2 envelope proteins and monoclonal antibodies thereto, as well their use in diagnosis, prophylaxis or treatment.[0003...

Claims

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