Compositions for preventing or ameliorating multiple risk factor syndromes
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preparation example 1
Preparation of a Licorice Hydrophobic extract-1
[0051] Using a glass vessel, 500 g of licorice powder (Kaneka Sun Spice Co., Ltd.) was steeped in 5 volumes of ethyl acetate and allowed to stand at room temperature, protected against light, for 1 week with occasional stirring. The mixture was filtered through filter paper (ADVANTEC No.2) twice to remove the powder and recover an extract solution. The extract solution was concentrated under reduced pressure to remove the solvent, whereupon 33.91 g of a licorice hydrophobic extract was obtained.
reference example 1
The Glycyrrhizin Content of the Licorice Hydrophobic Extract
[0052] The glycyrrhizin content of the licorice hydrophobic extract prepared in Preparation Example 1 was quantitated by HPLC referring to the method of M. A. Raggi, et al. (Boll. Chim. Farmaceutico., 133, 704-708, 1994). As the analytical column, COSMOSIL 5C18-AR, 4.6.times.250 mm (Nacalai Tesque, Inc.) was used at 40.degree. C. Using acetonitrile:distilled water:acetic acid (35:65:0.5, v / v) as a mobile phase run at a flow rate of 1 ml / min., the peak was detected at 251 nm. Using ammonium glycyrrhizinate (Wako Pure Chemical Industries, Ltd.; food additive test grade) as a glycyrrhizin standard, a calibration curve was constructed over the concentration range of 1 to 20 .mu.g / ml, and an assay was carried out.
[0053] As a result, the amount of glycyrrhizin in 1 mg / ml of the licorice hydrophobic extract was 4 .mu.g / ml, i.e. the glycyrrhizin content of the extract was 0.4%. Since the glycyrrhizin content is generally not less t...
preparation example 2
Preparation of a Licorice Hydrophobic Extract-2
[0065] Using a glass vessel, 300 g of licorice powder (Kaneka Sun Spice Co., Ltd.) was steeped in 10 volumes of lukewarm water (about 40.degree. C.) and stirred, protected against light, overnight (for about 18 hours). The mixture was then filtered through filter paper (ADVANTEC No.2) and the residue obtained was dried (about 60.degree. C.) to recover 265 g of an aqueous extraction residue. This residue was steeped in 5 volumes of ethanol and allowed to stand at room temperature, protected against light, for 1 week with occasional stirring. The mixture was filtered through filter paper (ADVANTEC No.2) twice to remove the powder and recover an extract solution. This extract solution was concentrated under reduced pressure to remove the solvent, whereupon 23.62 g of a licorice hydrophobic extract was obtained.
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