Methods and reagents for treating honeybees for parasitic mites

Inactive Publication Date: 2006-01-12
SWANSON MELVIN J
View PDF8 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0014] In one preferred embodiment, the inventive compositions and methods provide less hazardous and more convenient techniques for treating honeybees, as well as greater control of the rate of release of the active agent. In preferred embodiments, the rate of release of the active agent is at least partly controlled by the polymer gel composition itself. This is in contrast to prior methods and compositions that rely upon packaging of a pest control composition to control release of the active agent. In preferred embodiments, the invention allows the option of formulating gels having different release rates for use under different conditions of weather and hive conditions, such as levels of mite infestation.
[0015] In another aspect of the invention, the active agent is incorporated into a covalently crosslinked polymer gel. The resulting gel can be cast into any desirable shape, for example, by polymerizing appropriate monomers in a solution containing the active ingredient and optional additives. According to this aspect of the invention, the active ingredient is provided in a mechanically stable, firm, and/or non-flowing state. This is in contrast to prior compositions that are provided in the form of a paste-like gel that is capable of flowing (and are thus more hazardous for the user and for shipping and handling). Moreover, the covalently crosslinked polymer gel formulation of the invention can, in preferred embodiments, allow greater control over rates of release of the active agent compared with liquid formic acid. Preferably, the inventive covalently crosslinked polymeric gel compositions do not require manipulation by the user to change physical or chemical characteristics of the composition, such as dilution of the composition prior to application to a hive (as can be required when liquid formic acid is used).
[0016] In preferred aspects, the covalently crosslinked polymer gel also provides an adva

Problems solved by technology

Parasitic mites have caused serious problems both for the beekeeping industry and more broadly in agriculture, since honeybees are important for pollination of many important crops.
Various treatments have been attempted to control parasitic mites in honeybees, although limitations of currently available treatments include toxicity of the chemicals used, marginal effectiveness, inconvenience of use, difficulties in registration of the composition with environmental agencies, mite resistance, and/or contamination of beeswax.
This product is stated to be only 75% effective, so does not provide a complete treatment.
Unfortunately, there are now strains of mites that are resistant to both fluvalinate and coumaphos.
Furthermore, there are concerns about contamination of honey and beeswax with these agents.
However, none of these materials is active against both types of parasitic mites.
Formic acid has been used quite effectively in much of the world to control mite infestation,

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

Synthesis of a Gel Containing Formic Acid and a Salt of Formic Acid

[0053] Sodium formate (18 gm, 265 mmole) and methylene-bis-acrylamide (BIS) (300 mg) were dissolved in 40 ml (1.06 mole) of formic acid and 20 ml of distilled water. To this solution was added 25 ml (365 mmole) of acrylic acid, 100 μl of N,N,N′,N′-tetramethylethylenediamine (TEMED) and 1.2 gm of ammonium persulfate. The final volume of the solution prior to polymerization was 91 ml. The solution was poured into a 10 cm×10 cm plastic box. Nitrogen was bubbled through the solution, then the box covered tightly and put into an oven at about 50° C. overnight to polymerize into a gel. The gel was removed from the box and overlaid on each side with pieces of paper towel cut to approximately the size of the gel and stored in a sealed container until used.

example 2

Testing of Formic Acid / Sodium Formate Gels in Active Beehives

[0054] Two gel slabs prepared by the procedure of Example 1 were placed in a beehive, consisting of three hive bodies, one at the top under an inner cover and one under a screen at the bottom of the hive. The formic acid vapor concentration was measured by inserting a tube into a hole drilled in the bottom hive body using a Draeger tube (Fisher, Cat. No. 17-985-234) and an Accuro Draeger pump Fisher, Cat. No. 17-986-70A) following the manufacturer's instructions. After 24 hours, the concentration was 23 ppm. The temperature was 68° F. After seven days, the concentration was 40 ppm. The temperature was 70° F. After 11 days, the concentration was 12 ppm when the temperature was 52° F. Two other hives were treated similarly with similar results.

example 3

Synthesis of a Gel Containing Formic Acid and Sodium Formate in Crosslinked Polyacrylic Acid for Laboratory Comparison

[0055] Methylene-bis-acrylamide (25 mg) was dissolved in 4.0 ml of formic acid. To that solution was added 2.0 ml of acrylic acid (29.2 mmole). In a separate solution, 1.0 gm of sodium formate and 125 mg of ammonium persulfate were dissolved in 1.0 ml of distilled water. The solutions were combined to achieve a final volume of 8.5 ml. The combined solution was poured into a plastic box. Nitrogen was bubbled through the solution, then the box covered tightly and put into an oven at about 50° C.-60° C. overnight to polymerize into a gel. After overnight in the oven, the gel was removed and covered on each side with paper towel pieces cut to approximately the dimensions of the gel and stored in a zip lock plastic bag until used.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides controlled release compositions for treatment of parasitic mites in honeybees, the compositions composed of a covalently crosslinked polymer gel and an active agent that includes a salt of formic acid. The invention also provides methods for controlling parasitic mites in honeybees, the methods involving treating the honeybees with a controlled release composition composed a covalently crosslinked polymer gel and an active agent that includes a salt of formic acid. The invention further provides a kit for treatment of parasitic mites in honeybees, the kit including an envelope that contains a controlled release composition of the invention. Optionally, the active agent can include formic acid.

Description

FIELD OF THE INVENTION [0001] The invention relates to compositions and methods for controlling infestations of honeybee colonies. More particularly, the invention relates to compositions and methods for treating honeybees for parasitic infestations, such as mite infestations. BACKGROUND OF THE INVENTION [0002] Parasitic mites have caused serious problems both for the beekeeping industry and more broadly in agriculture, since honeybees are important for pollination of many important crops. There are two types of parasitic mites that cause problems for honeybees. Both Varroa jacobsoni and the tracheal mite Acarapis woodi cause infestations that have resulted in extensive reductions in honeybee populations. The successful management of honeybees in the face of these pests requires multiple approaches for control, including breeding of resistant strains and various treatments and control measures. [0003] Various treatments have been attempted to control parasitic mites in honeybees, al...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01K51/00
CPCA01N37/02A01N25/18A01N25/10A01N2300/00
Inventor SWANSON, MELVIN J.
Owner SWANSON MELVIN J
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products