Cleavage of nucleic acids

a nucleic acid and cleavage technology, applied in the field of nucleic acid cleavage, detection and characterization of nucleic acid sequences and sequence changes, can solve the problems of ineffective thermostable dna ligases, limited use of lcr for mutant screening, and inability to raise reaction temperatures to prevent, etc., to achieve rapid detection and identification of variants
US20060035256A1Inactive Publication Date: 2006-02-16THIRD WAVE TECH
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
THIRD WAVE TECH
Publication Date
2006-02-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.
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Description

[0001] The present application is a Continuation of co-pending U.S. application Ser. No. 09 / 940,925, filed Aug. 28, 2001, which is a Divisional application of U.S. application Ser. No. 09 / 655,378, filed Sep. 5, 2000, now U.S. Pat. No. 6,673,616, which is a Continuation of U.S. application Ser. No. 08 / 520,946, filed Aug. 30, 1995, now U.S. Pat. No. 6,372,424, which is a Continuation-in-Part application of U.S. appliation Ser. No. 08 / 484,956, filed Jun. 7, 1995, now U.S. Pat. No. 5,843,654, which is a Continuation-in-Part of U.S. application Ser. No. 08 / 402,601, filed Mar. 9, 1995, now abandoned, which is a Continuation-in-Part of U.S. application Serial No. 08 / 337,164, filed Nov. 11, 1994, now abandoned, which is a Continuation-in-Part of U.S. application Serial No. 08 / 254,359, filed Jun. 6, 1994, now U.S. Pat. No. 5,614,402, which is a Continuation-In-Part Application of U.S. application Ser. No. 08 / 073,384, filed Jun. 4, 1993, now U.S. Pat. No. 5,541,311, which is a Continuation-In...

Claims

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