Commercial production of polysaccharide degrading enzymes in plants and methods of using same

a technology of polysaccharide degrading enzyme and plant, which is applied in the field of commercial production of heterologous proteins in plants, can solve the problems of crippling the ability of the economy to function, affecting the standard of living of the population, and consuming over 100 billion gallons of gasoline per year in the transportation sector alon
US20090205086A1Inactive Publication Date: 2009-08-13APPLIED BIOTECH INST
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
APPLIED BIOTECH INST
Publication Date
2009-08-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

Expression of recombinant polysaccharide degrading enzymes in plants is described. In one embodiment, expression of the enzyme is preferentially directed to the seed of the plant. Expression may also be preferentially targeted to specific locations within the plant cell. Expression of cellulases in corn is shown. The result is the capacity to produce polysaccharide degrading enzymes in plants at commercially acceptable levels in a reliable manner. Methods of using same in production of ethanol is also described, including use of the plant-produced enzymes in the ethanol production process.
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Description

[0001] This application is a continuation of previously filed and co-pending application U.S. Ser. No. 11 / 219,180, filed Sep. 2, 2005, which claims benefit under 35 U.S.C.§119(e) to previously filed and co-pending application U.S. Ser. No. 60 / 607,098, filed Sep. 3, 2004 and which application U.S. Ser. No. 11 / 219,180 is also a continuation-in-part of U.S. Ser. No. 10 / 310,292, filed Dec. 5, 2002, which claims benefit under 35 U.S.C.§ 119(e) to U.S. Ser. No. 60 / 340,035, filed Dec. 6, 2001, the contents of all such prior filings are incorporated herein by reference.GOVERNMENT INTEREST

[0002] The work of this invention was funded in part by a grant from the USDA and the government has certain rights therein.FIELD OF THE INVENTION

[0003] The present invention relates to commercial production of heterologous proteins in plants. More specifically, the invention is to novel methods of expressing a heterologous polysaccharide degrading enzyme in plants, particularly in grains, and to methods of ta...

Claims

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