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Porous polysaccharide scaffold comprising nano-hydroxyapatite and use for bone formation

a technology of nano-hydroxyapatite and polysaccharide, which is applied in the field of porous polysaccharide scaffold comprising nano-hydroxyapatite and bone formation, can solve the problems of autologous and allograft bones, secondary trauma, and immune repulsion of allograft bones, and achieve the effect of facilitating vasculature growth

Inactive Publication Date: 2013-08-29
INST NAT DE LA SANTE & DE LA RECHERCHE MEDICALE (INSERM) +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent describes a scaffold made of a porous polysaccharide that has been colored to make it easier to see. The colorant can be a dye, pigment, or a natural coloring agent like alcian blue, fluorescein isothiocyanate (FITC), or FITC dextran. The technical effect of this invention is that it provides a better way to see and locate the scaffold in various applications such as medical implants or drug delivery systems.

Problems solved by technology

However, the use of autologous bone is known to result in secondary trauma and allograft bone induces immune repulsion.
In addition, autologous and allograft bones present serious limitations since their uses are dependent on the size and the localisation of the defect.
For example, it was reported that grafts in large defects were resorbed by the body before the completion of osteogenesis, which leaves a doubt about the success of this therapy (Hoexter D L.
However, using said scaffolds for the treatment of bone related disorders still present various drawbacks related to the disease to be treated, as it depends on the type, size, and localisation of the damaged bone, as well as on the nature, age and sex of the subject to be treated.
However, the use of hydroxyapatite presents limitations since it is mainly effective on osseous sites.
There is currently no available technique providing bone formation which does not present any risk of rejection and which may be independent of the size and localisation of the bone to regenerate.

Method used

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  • Porous polysaccharide scaffold comprising nano-hydroxyapatite and use for bone formation
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  • Porous polysaccharide scaffold comprising nano-hydroxyapatite and use for bone formation

Examples

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Effect test

example

Example 1

Implantation of the Scaffold of the Invention in Calvaria Site of Athymic Mice

Materials and Methods

Nano-Hydroxyapatite Preparation

[0131]Nano-hydroxyapatite (n-HA) was prepared by wet chemical precipitation using a 0.6M solution of Phosphoric acid (H3PO4 Rectapur, Prolabo®, France) and a 1M solution of calcium hydroxide (CaOH2 Alfa Aesar, Germany). 100 ml of H3PO4 solution were added dropwise in 100 ml of CaOH2 solution during 30 minutes under vigorous stirring at room temperature. At the end of reaction, pH was adjusted to 9 using 0.4.10 mol of a 0.6 M sodium hydroxide solution, then stirring was continued during 12 hours.

[0132]Nano-hydroxyapatite (n-HA) has been characterized by transmission electron microscopy (TEM), scanning electron microscopy and by FTIR analysis. TEM revealed n-HA needle-shaped crystals of 50 nm long. FTIR analysis showed specific bands of phosphate ions of at 559 cm−1, 601 cm−1 and 1018 cm−1 and a non-specific carbonate band 1415 cm−1.

Preparation of ...

example 2

Implantation of the Scaffold According to the Invention in a Non Osseous Site in Mice and Osseous Site in Rat

Materials and Methods

[0145]Nanohydroxyapatite and scaffold according to the invention were prepare as described in Example 1. The inventors assessed the implantation of said scaffold in animal. Both the procedure and the animal treatment complied with the Principles of Laboratory Animal Care formulated by the National Society for Medical Research. The studies were carried out in accredited animal facilities at the University of Bordeaux Segalen, under authorization (N°: 3300048 of the Ministere de l'Agriculture, France) and were approved by the Animal Research Committee of Bordeaux University.

Non-Osseous Implantation in Mice: Ectopic Bone Formation Analysis

[0146]The two different formulations of scaffolds: disk-shaped matrices without n-HA (Group 1) and the composite scaffold containing n-HA (MATRI+) (Group2) (cylinders of 4 mm diameter and 6 mm depth) were inserted into subc...

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Abstract

The present invention relate to three dimensional porous polysaccharide matrices able to induce mineralisation of a tissue in osseous site, as well as in non-osseous site, in the absence of stem cells or growth factors.

Description

FIELD OF THE INVENTION[0001]The present invention relates to a method for preparing a porous polysaccharide scaffold comprising hydroxyapatite, preferably nano-hydroxyapatite, that supports mineralization of tissues. The present invention further provides a porous polysaccharide scaffold obtainable by said method, and its use for bone formation.BACKGROUND OF THE INVENTION[0002]The topic of bone-related disorders has gained considerable attention over the past years. The use of autologous and allograft bones has been popularly implemented in clinics for overcoming bone related disorders, such as bone defect.[0003]However, the use of autologous bone is known to result in secondary trauma and allograft bone induces immune repulsion. In addition, autologous and allograft bones present serious limitations since their uses are dependent on the size and the localisation of the defect. For example, it was reported that grafts in large defects were resorbed by the body before the completion ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61L27/46A61L27/56
CPCA61L27/20C08B37/0021A61L27/56A61L2400/12A61L2430/02C08J2205/022C08J2207/10C08J2305/00C08J2405/02C08J9/0061C08J9/26C08J9/28C08J2201/026C08J2201/0444C08J2201/0484C08B37/0018A61L27/46A61L2400/08A61P19/00A61L27/12A61L2300/112
Inventor AMEDEE, JOELLELETOURNEUR, DIDIERLE VISAGE, CATHERINEDERKAOUI, SIDI MOHAMMEDFRICAIN, JEAN-CHRISTOPHECATROS, SYLVAIN
Owner INST NAT DE LA SANTE & DE LA RECHERCHE MEDICALE (INSERM)