Carotenoid Compound Coming From Plant And Containing Natural Astaxanthin, Preparation Method Therefor, And Composition

Abstract

The present invention provides a high-content carotenoid compound from adonis amurensis. The content of the total carotenoid of the carotenoid compound is greater than 95%. The content of all-trans (3S, 3′S)-carotenoid is greater than 80%. The carotenoid crystals have a high purity, and can be used in multiple forms in the fields of a dietary supplement of a human being, a food additive, a feed additive and a cosmetic product. In addition, the present invention also provides a method for manufacturing the compound.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a national phase application of PCT / CN2014 / 000862, filed on Sep. 23, 2014, which claims priority to Chinese Application No. 201310440119.6 filed on Sep. 24, 2013.FIELD OF THE INVENTION[0002]The present invention relates to a carotenoid compound coming from plant extract with less impurity containing natural astaxanthin with high biological activities, and its preparation method and composition, and relates to the field of biochemistry.BACKGROUD OF THE INVENTION[0003]Astaxanthin's formula is C40H52O4 and is referred to as 3,3′-dihydroxy-4,4′-diketo-β,β′-carotene, widely exists in nature, is an important substance of carotenoid, and has a higher physiological activity than other carotenoids. The molecular formula is as follows:[0004]As for a kind of natural preparation, astaxanthin has broader uses and development prospects in various industries such as pharmaceuticals, aquaclutures, foods, chemistries.[0005]Astaxanthin ...

AI Technical Summary

Benefits of technology

[0047]Preferably, the concentration of the alkali solution of the reaction mixture in step (b) is 0.01˜2.50 mol / L after completion of adding dropwise the alkali solution. More preferably, the concentration of the alkali solution of the reaction mixture in step (b) is 1.00˜2.00 mol / L after completion of adding dropwise the alkali solution. The alkali concentration of the reaction system is critical, because firstly the saponification is not complete if the alkali concentration is low, and it takes a long reaction time; secondly, it is easy to produce oxidation of astaxanthin to semi-astancene and atacene, to reduce purities of astaxanthin in final products if alkali concentration is too high. Consequently, the alkali concentration of the reaction system of the present invention is 0.01˜2.50 mol / L, preferably, 1.00˜2.00 mol / L. Some general separation measures such as using filter aids, changing the acidity-basicity and other measures in the process are used for increasing separation efficiency.

[0050]The application form of astaxanthin may be in the form of oil, that is, the astaxanthin is dispersed in vegetable oil. And the application form of astaxanthin also may be in the form of powder, that is, after mixing with one or more selected from gelatin, modified starch, dextrin, sucrose and other auxiliary materials, the astaxanthin is embedded by a microcapsule emulsion technology in order to obtain astaxanthin in the form of microcapsule powder with good stability.

Problems solved by technology

Astaxanthin prepared by chemical synthesis is expensive and has significant differences in molecular structures, biological functions, application effects and biological safeties.

Synthetic astaxanthin obviously has stability and antioxidant activity lower than that of natural astaxanthin, and the effects of biological absorption and pigmentation of synthetic astaxanthin are also worse than that of natural astaxanthin.

And synthetic astaxanthin can not be converted into natural astaxanthin in animal bodies.

On the other hand, pollutions and impurities are inevitably produced in the process of synthesis, which can lead to generation of some potential safety hazard and reduction of safety performance.

Wherein the bacteria thalli has basically no use value because of its slow growth and low synthesis.

However, it is difficult to establish a stable and efficient production technology system, because the Haematococcus pluvialis is sensitive to environment, and susceptible to protozoa and bacterial contamination.

Consequently it increases production costs and is adverse to commercial production.

Many factors restrict astaxanthin extraction including shellfish waste such as shrimp shell being perishable, large volume, relatively high transportation cost, low content levels of astaxanthin with large changes, binding state of astaxanthin, particle size shell, etc.

No better method with industrial scales of producing astaxanthin from these wastes has been found at present.

However, it would be difficult to realize large-scale production because of some deficiencies such as culture conditions, high costs, and difficult extraction conditions.

Astaxanthin is a very unstable substance, with high antioxidant activity, and is very easily oxidized to semi-astacene under the conditions of alkaline environment or oxygen or heating, thereby affecting its bioactivity.

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