Broad Spectrum Biopesticides Comprising Beneficial Microorganisms
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Metarhizium Anisoplae Using Solid-State Fermentation
[0233]Metarhizium anisopliae was grown by solid-state fermentation using a medium comprising a mixture of rice bran and rice husk with initial water content fixed at a value of about 50%. The medium was spread into metal trays fitted for a standard proofing oven (i.e., used by commercial bakers). Fermentation was then performed in a proofing oven at a temperature of 27±1° C. Fermentation time was 12-14 days.
Example 2—Growth of Beauveria Bassiana Using Solid-State Fermentation
[0234]A seeding culture of Beauveria bassiana with concentration of 1×107 conidia / g was grown for two days in a 3% corn meal, 2% rice bran, and 2% corn steep powder medium.
[0235]The seed culture was added to a wet rice medium (polished white rice, 40% moisture content), with the amount of inoculum equaling 10% of the total amount of medium. The culture was grown in a polyethylene bag.
[0236]Using these optimal conditions, 4.05 grams of conidia / 100 grams dry rice...
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