Method to clean and disinfect pathogens on the epidermis by applying a composition containing peroxidase, an iodide compound, a peroxide and a surfactant
a technology of epidermis and composition, applied in the field of epidermis cleaner, can solve problems such as problematic formulation of skin cleaning compositions containing conventional antiseptic agents, and achieve the effect of reducing the number of viable aspergillus fumigatis
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example 1
Component A, the peroxidase containing component, consisted of 0.4 mg / ml of Sigma Type I peroxidase, 4 mg / ml acetylated BSA, 2 mg / ml sodium iodide, 0.2 mg / ml calcium chloride, 1 mM tris-(hydroxymethyl)aminomethane (pH 7.2), and 20% glycerol. Component B, the peroxide containing component, consisted of 0.0038% hydrogen peroxide, 2.5% sodium lauryl sulfate, 0.125 mg / ml ethylenediaminetetraacetic and 0.125 mg / ml of sorbic acid. Four ml of component B was added to 1 ml of several phosphate buffers each of which was 0.4 molar. The pH of the 0.4 molar phosphate buffers was 4.0, 4.5, 5.5 and 6.5.
Cultures of Listeria selegeri, E. coli, and Salmonella typhimurium were spun down in a clinical centrifuge and washed in normal saline. Equal volumes of component A (1 ml) and component B (1 ml) were added to 1 ml of these bacterial suspensions and mixed. Aliquots were withdrawn every 20 seconds and diluted in 10 mg / ml sodium fluoride. Serial dilutions of each time point were made and the CFU per m...
example 2
Component A, the peroxidase containing component, consisted of 1.0 mg / ml of Sigma Type I peroxidase, 30% sucrose, 6 mg / ml sodium iodide, 1.0 mg / ml calcium chloride, 5 mM tris-(hydroxymethyl)aminomethane (pH 7.5), and 4 mg / ml sodium chloride. Component B, the peroxide containing component, consisted of 0.0030% hydrogen peroxide, 1.0% cetyl alcohol, and 1.0% .[.Brij-35.]. .Iadd.BRIJ-35. .Iaddend.Four ml of component B was added to 1 ml of several phosphate buffers each of which was 0.3 molar. The pH of the 0.3M phosphate buffers was 4.0, 4.5, 5.5 and 6.5.
Cultures of Staphlococcus aureus, Staphlococcus epidermitis, and Salmonella typhimurium and Listeria selegeri were spun down in a clinical centrifuge and washed in normal saline Equal volumes of component A (1 ml) and component B (1 ml) were added to 1 ml of these bacterial suspensions and mixed. Aliquots were withdrawn every 20 seconds and diluted in 10 mg / ml sodium fluoride. Serial dilutions of each time point were made and the CFU ...
example 3
The effect of pH between 2 0 and 7.0 on the inactivation of Aspergillus fumigatis with a disinfecting epidermal skin cleaner was examined. The peroxidase component (component A) contained 1.0 mg / ml of sodium iodide, 20.0 percent glycerol, 5.0 mg / ml of sodium chloride, 1.1 mg / ml of calcium chloride, 0.5 mg / ml of peroxidase (Sigma Type I) in 10 mM tris-(hydroxymethyl)aminomethane. The peroxide component (component B) contained 0.03 percent hydrogen peroxide, 1.0 mg / ml of ethylenediaminetetraacetic, and 1.8 percent of sodium-lauryl-sulfate in water. Immediately prior to use, component B was mixed with 0.20 mol / L buffers which had been equilibrated at the desired pH values. For a pH value in the disinfecting admixture of 7.0, 6.5, and 6.0, sodium phosphate was used as the buffer for mixture with component B. For a pH value in the disinfecting admixture of 5.0, and 4.0, citric acid - sodium phthalate was used as the buffer for mixture with component B. For a pH value in the disinfecting ...
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