Renaturation of reconstituted human bone protein-1 and making method of its preparation

A technology of osteogenic protein and preparation, which is applied in the preparation of osteogenic protein-1 preparation and recombinant human osteogenic protein-1 preparation, which can solve the problems of high cost, low output and limited use, and achieve the renaturation process easy-to-use effects
CN100336825CInactive Publication Date: 2007-09-12SHANDONG MEDICAL BIO TECH RES CENT
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANDONG MEDICAL BIO TECH RES CENT
Publication Date
2007-09-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses a recombinant human osteoplastic protein-1 renaturation and its preparation preparation method. It utilizes chromatography to obtain rh OP-1 monomer protein whose purity is above 95%. Said invention also provides the concrete steps of said preparation method, including: in 2M-6M urea or 1M-4M guanidine hydrochloride buffer solution making dialysis and renaturation, pH value of renaturation buffer solution is 8.0-10, renaturation temperature is room temperature or 4deg.C and renaturation time is 24-96 hr, then further making chromatographic purification, dialysis, sterilization, packaging and freeze-drying so as to obtain the renaturation preparation of recombinant human osteoplastic protein-1. Besides, said invention also provides the application method of said renaturation preparation of recombinant human osteoplastic protein-1.
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Description

Technical field:

[0001] The invention relates to a preparation method of osteogenic protein-1, in particular to a preparation method of recombinant human osteogenic protein-1 (rhOP-1). It uses genetic engineering means to express and mass-produce recombinant human osteogenic protein-1 mature peptide in Escherichia coli to refold recombinant human osteogenic protein-1 and prepare its preparations. After sterilization, the preparation prepared by the invention can be clinically used for repairing and orthopedic bone defects, and for maintaining and recovering alveolar bone in oral cavity tooth extraction wounds and the like. It belongs to the field of biotechnology and pharmaceuticals. Background technique:

[0002] With the rapid development of genetic engineering technology, more and more people provide some protein and polypeptide products for clinical and industrial production through the heterologous expression of protein. So far, E.coli is still the preferred expressio...

Claims

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