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Method for rapid selection of plant disease-resistant fungi marine microorganism

A technology for marine microorganisms and plant diseases, which is applied in the fields of biochemical equipment and methods, and the determination/inspection of microorganisms. Simple and easy to implement, the effect of promoting work efficiency

Inactive Publication Date: 2007-12-05
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In agricultural production, some fungi have disease effects on crops, causing great harm to agricultural production; toxins produced by Penicillium purpura have toxic effects on soybean growth, reducing soybean production and causing great losses
In the prior art, for harmful fungi, people are constantly looking for microorganisms and active substances produced by them in nature that can kill or antagonize harmful fungi. Harmful fungi are often used as target bacteria to participate in the screening and detection process, but the vast majority Due to the characteristics of slow growth (48-72hr) and easy diffusion (producing flying spores, etc.), most harmful fungi are time-consuming, laborious and inefficient in actual detection, and sometimes affect the timeliness of test results and cause laboratory pollution, which is very annoying Headache, the limitations and advances of the method are obvious

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1. Selection of rapid screening indicator bacteria

[0015] Select 28 strains of harmless, fast-growing Candida rugosa and three strains of plant disease fungi, Penicillum purpurogenum, Fusarium solani and Botrytis fuckeliana, and use nystatin (also Parallel antifungal tests can be carried out for other antifungal drugs) to determine the correlation between the response and sensitivity of plant disease fungi and harmless yeasts to biological substances; among them, 3 strains of Candida rugosa and Penicillium purpurea are effective against mold-forming fungi They have the same reaction (parallel antifungal tests on Candida rugosa strains and plant disease fungi with antifungal antibiotics, and they will have inhibition zones at the same time), so they can be selected as rapid screening indicators for Candida rugosa strains bacteria;

[0016] 1) Preparation of indicator bacteria (testing bacteria culture)

[0017] Take a ring of yeast culture agar slant seeds and inocul...

Embodiment 2

[0028] The difference from Example 1 is that the rapid screening of plant disease-resistant marine microorganisms,

[0029] 1) Preparation of test bacteria

[0030] 71 strains of marine spores were isolated from coastal samples in China, and the purified marine spores were spread on a solid plate suitable for growth, and cultured at 28°C for 1 day before use;

[0031] Detection of double-layer culture: liquefy 200ml of Y76 solid medium and pour the bottom layer (wooden frame or stainless steel frame), after condensation, put the solid Y76 medium mixed with Y76 yeast kept in a 54°C water bath (add 0.3ml per 50ml medium Liquid culture), pour the upper layer, after waiting for coagulation, put the sample to be tested into the marine spore agar block, 28 ℃ static culture 10-24hr, obtain 9 strains of marine spores with antibacterial activity.

Embodiment 3

[0033] Detection of strains screened out:

[0034] 47 strains of marine bacteria and marine streptomyces with antagonistic effects were screened with Candida as the target bacteria, and the antibacterial activities against Penicillium purpura and Candida were determined in parallel to determine the response of the target bacteria and Penicillium purpura to the screened strains and sensitivity correlation, the measurement results showed that the screened strains also had inhibitory effect on Penicillium purpura; the bacteriostatic tests were carried out on the plant pathogens Fusarium and Botrytis with the screened strains, and the results were also effective.

[0035] One of these, the Candida rugosa strain Y76, worked extremely well.

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Abstract

The invention relates to a method for rapid selection of plant disease-resistant fungi marine microorganism which comprises, (1) conducting parallel bacteriostasis experiment with Candida rugosa strain and plant disease resistant fungous antibiotics, choosing Candida rugosa strain capable of serving as indicating bacterium, (2) using the Candida rugosa strain obtained in step (1) as indicating bacterium to screen ocean microorganism with inhibitory actions.

Description

technical field [0001] The invention relates to the screening of marine microorganisms resistant to plant disease fungi, in particular to a method for rapidly screening marine microorganisms resistant to plant disease fungi. Background technique [0002] In agricultural production, some fungi have disease effects on crops, causing great harm to agricultural production; toxins produced by Penicillium purpura have toxic effects on soybean growth, reducing soybean production and causing great losses. In the prior art, for harmful fungi, people are constantly looking for microorganisms and active substances produced by them in nature that can kill or antagonize harmful fungi. Harmful fungi are often used as target bacteria to participate in the screening and detection process, but the vast majority Due to the characteristics of slow growth (48-72hr) and easy diffusion (producing flying spores, etc.), most harmful fungi are time-consuming, laborious and inefficient in actual dete...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/18
Inventor 马成新胡江春薛德林刘全永王书锦
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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