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Enzyme function sensitive membrane containing stripped MnO2 for biosensor and preparation method thereof

A biosensor, manganese dioxide technology, applied in biochemical equipment and methods, immobilized enzymes, determination/inspection of microorganisms, etc. Effect of chemical behavior and response sensitivity improvement

Inactive Publication Date: 2008-01-02
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the sensitivity and application range of reagent-free electrochemical biosensors still have certain limitations, mainly because: (1) there is a lack of simple and efficient methods to immobilize biologically active substances; (2) most biologically active substances (such as enzymes) , cells, etc.) to a certain extent, the active center is covered by the protein, and the direct electron transfer between the biologically active center and the electrode is relatively difficult.

Method used

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  • Enzyme function sensitive membrane containing stripped MnO2 for biosensor and preparation method thereof
  • Enzyme function sensitive membrane containing stripped MnO2 for biosensor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A Preparation of exfoliated manganese dioxide sol

[0043] A-1. 200mL containing 0.6mol / L NaOH and 1.2mol / L H 2 o 2 The mixed solution was quickly added to 100mL containing 0.3mol / L Mn(NO 3 ) 2 solution, vigorously stirred and reacted for 20 minutes, filtered, transferred the filter cake to a polytetrafluoroethylene cup, added 30mL NaOH solution with a concentration of 2mol / L, stirred until it became a paste, sealed the polytetrafluoroethylene cup in water In a hot kettle, hydrothermal treatment was carried out at 150°C for 20 hours. The hydrothermal kettle was naturally cooled to room temperature, the kettle was opened for suction filtration, and the filter cake was washed with deionized water until the pH of the filtrate was 8. The filter cake was dried in an air atmosphere at 70° C. for 9 hours to obtain layered manganese dioxide.

[0044] A-2. Add 2.6g of the above layered manganese dioxide solid powder to 300mL of HNO with a concentration of 1mol / L 3 solution...

Embodiment 2

[0050] A Preparation of exfoliated manganese dioxide sol

[0051] A-1. 200mL containing 0.8mol / L NaOH and 1.2mol / L H 2 o 2 The mixed solution was quickly added to 100mL containing 0.4mol / L Mn(NO 3 ) 2 solution, vigorously stirred and reacted for 30 minutes, filtered, transferred the filter cake to a polytetrafluoroethylene cup, added 50mL NaOH solution with a concentration of 3mol / L, stirred to form a paste, sealed the polytetrafluoroethylene cup in water In a hot kettle, hydrothermal treatment was carried out at 160°C for 15 hours. The hydrothermal kettle was naturally cooled to room temperature, the kettle was opened for suction filtration, and the filter cake was washed with deionized water until the pH value of the filtrate was 9. The filter cake was dried in an air atmosphere at 80° C. for 6 hours to obtain layered manganese dioxide.

[0052] A-2. Add 2.5g of the above layered manganese dioxide solid powder to 280mL of HNO with a concentration of 1.5mol / L 3 solution...

Embodiment 3

[0058] A Preparation of exfoliated manganese dioxide sol

[0059] A-1. 200mL containing 0.6mol / L NaOH and 1.5mol / L H 2 o 2 The mixed solution was quickly added to 100mL containing 0.4mol / L Mn(NO 3 ) 2 solution, vigorously stirred and reacted for 30 minutes, filtered, transferred the filter cake to a polytetrafluoroethylene cup, added 40mL of NaOH solution with a concentration of 3mol / L, stirred to form a paste, sealed the polytetrafluoroethylene cup in water In a hot kettle, hydrothermal treatment was carried out at 160°C for 18 hours. The hydrothermal kettle was naturally cooled to room temperature, the kettle was opened for suction filtration, and the filter cake was washed with deionized water until the pH of the filtrate was 8. The filter cake was dried in an air atmosphere at 80° C. for 8 hours to obtain layered manganese dioxide.

[0060] A-2. Add 2.5g of the above-mentioned layered manganese dioxide solid powder to 300mL of HNO with a concentration of 1.2mol / L 3 s...

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Abstract

The invention relates to an electrochemistry biological sensor enzyme function sensing film with manganese dioxide and it's preparing method. The sensing film is formed by high molecular material of oxidative enzyme and fixed enzyme and manganese dioxide. The preparing method comprises: mixing the manganese dioxide sol and the oxidative enzyme, coating it on the cleaning glass carbon electrode surface, immersing it into the macromolecule gel after drying it, volatilizing the weld flux at normal temperature and forming a layer of enzyme function sensing film with manganese dioxide on the glass carbon electrode surface.

Description

Technical field: [0001] The invention belongs to the technical field of electrochemical biosensor and its preparation, in particular to an electrochemical biosensor enzyme function sensitive membrane containing exfoliated manganese dioxide and a preparation method thereof. Background technique: [0002] Electrochemical biosensors have the characteristics of stable performance, high selectivity, low price, easy operation, and easy miniaturization, and have been widely used in clinical diagnosis, industrial control, food testing, drug analysis, environmental analysis, biochips, and military fields. widely used. Among them, the third-generation reagent-free electrochemical biosensors, which use the direct electrochemical behavior (ie, direct electron transfer) between bioactive substances and electrodes for detection, have attracted more attention and are currently a hot spot in the field of electrochemical biosensor research. [0003] However, the sensitivity and application ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327C12Q1/00C12N11/00
Inventor 杨文胜吴金玲张熊段雪
Owner BEIJING UNIV OF CHEM TECH