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Chloride ion resistant strain S616 and its screening method

A technology of S616 and strains, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of high organic matter, high concentration of chloride ions, weak research, etc., and achieve efficient degradation effect

Inactive Publication Date: 2008-05-07
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the one hand, this type of wastewater has complex components, many refractory organic substances, and poor biodegradability of the wastewater; on the other hand, the wastewater has a high salt content and a high concentration of chloride ions, and the concentration of chloride ions in the mixed wastewater can reach 20,000 mg / L
At present, non-biological methods and biological methods are used for salt-containing organic wastewater. The non-biological method is unacceptable to enterprises due to its high treatment cost and poor treatment effect. The biochemical method has always been the first method in the water treatment process, mainly using A-B two-stage contact Oxidation method, traditional activated sludge method, SBR method, biological filter, anaerobic filter, etc., but most of the above biological methods can only treat wastewater with a salt content of less than 3%, while for high-salt wastewater (salt content 5% %, even 20%) are difficult to handle and therefore require special microorganisms
In recent years, foreign countries have reported reports on the breeding of salt-tolerant (NaCl) bacteria to treat high-salt organic wastewater. 2 ), complex waste water containing refractory substances (saponin), which is still a weak field of research

Method used

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  • Chloride ion resistant strain S616 and its screening method
  • Chloride ion resistant strain S616 and its screening method
  • Chloride ion resistant strain S616 and its screening method

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] 1. Screening method for a chlorine-resistant bacterial strain S616:

[0033] (1) Material preparation:

[0034] 1. Bacterial source and experimental wastewater:

[0035] (1) Bacterial source: activated sludge in the biochemical system for treating saponin wastewater;

[0036] (2) Experimental waste water (i.e. mixed saponin waste water): taken from the saponin production waste water of Fangkun Real Estate Co., Ltd., Shiyan City, Hubei Province; after internal electrolysis and CaO deployment, the specific indicators are: pH = 7.5-8.2, COD = 4000 -17000mg / L, Cl - =8000-30000mg / L, NH 4 -N 140-300mg / L.

[0037] 2. Culture medium:

[0038] Separation medium: CH 3 CH 2 COONa 30mmol, CH 3 CH 2 CH 2COONa 30mmol, CH 3 CHOHCOONa30mmol, yeast 2.0g, MgCl 2 0.1g, NH 4 Cl 1.0g, K 2 HPO4 0.4g, resazurin 0.002g, cysteine ​​0.5g, distilled water 1000ml, pH=7.0~73.

[0039] Screening medium: MgSO4 7H 2 O, 0.2g / L, KH 2 PO 4 0.5g / L, K 2 HPO 4 1.5g / L, NH 4 Cl 0.5 / L, ...

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Abstract

The invention relates to a chlorine-resistant microorganism of strain S616, wherein the bacterial strain being Bacillus firmus S616 (CCTCC No: M205146). The bacterial colony shape and main biochemical characters are also described in the specification, the invention can be applied to the highly effective degradation of high concentration organic contaminant.

Description

technical field [0001] The invention relates to a chlorine-resistant microorganism and a screening method thereof. Background technique [0002] High-salinity refractory organic wastewater, such as petroleum exploration, chemical, pharmaceutical and other wastewater, has become a worldwide technical problem in the field of environmental protection water treatment. However, the hydrochloric acid method turmeric saponin production wastewater is representative of the high-concentration saline refractory organic wastewater. On the one hand, this type of wastewater has complex components, many refractory organic substances, and poor biodegradability of the wastewater; on the other hand, the wastewater has a high salt content and a high concentration of chloride ions, and the concentration of chloride ions in the mixed wastewater can reach 20,000 mg / L. At present, non-biological methods and biological methods are used for saline organic wastewater. The non-biological method is un...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F1/58C12R1/07
Inventor 信欣王焰新鲍建国刘慧杨雪芬李平
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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