Unlock instant, AI-driven research and patent intelligence for your innovation.

Fatly phospho embrella Pa-I.H bacterial strain and method of culturing fatty phospho embrella and medicine made from its active component

A strain and mycelium culture technology, applied in the direction of organic active ingredients, microbial-based methods, medical preparations containing active ingredients, etc., to achieve the effects of stable metabolites, high safety, and reasonable process routes

Inactive Publication Date: 2008-05-14
SHANGHAI HI TECH UNITED BIO TECHCAL RES
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching, no literature reports were found

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fatly phospho embrella Pa-I.H bacterial strain and method of culturing fatty phospho embrella and medicine made from its active component
  • Fatly phospho embrella Pa-I.H bacterial strain and method of culturing fatty phospho embrella and medicine made from its active component
  • Fatly phospho embrella Pa-I.H bacterial strain and method of culturing fatty phospho embrella and medicine made from its active component

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0146] Cultivation of slant strains: PDA comprehensive medium, PH6.5, culture temperature 24-26°C, the bacteria germinate quickly, within 2-3 days after inoculation, obvious colonies based on the inoculated block will be formed for about 10-12 days Colonies overgrow the tube. The hyphae are white, cotton-wool-like, with many branches at the beginning, and light yellow at the later stage. Through microscopic examination, it is obvious that the mycelium is thick and branched, the lock-shaped joints are well developed, and the coloring of methylene blue single staining is deep, and a large number of growth points can be seen. Cavitation, no bacterial contamination.

[0147] Shake flask seed culture, the medium components used are peptone 1.5%, yeast extract 0.1%, maltose 1%, glucose 1%, wheat bran 5%, KH 2 PO 4 0.15%, MgSO 4 ·7H 2 O 0.075%, vitamin B 1 0.001%, PH6.5 (before elimination), autoclave pressure 1kg / cm 2 30min, inoculate after cooling, the inoculum amount is 1:...

Embodiment 2

[0153] Weigh 1Kg of the polylipid phosphocarp fruiting body, add 20Kg of water, and extract repeatedly at 95°C for 3 times, each time for 1 hour. The extracts were combined, filtered, concentrated in vacuo under reduced pressure, and the thermal specific gravity was 1.04, and finely filtered. Add edible ethanol to precipitate, and the final concentration reaches 70%. Filter to collect the precipitate and dissolve in water. Centrifuge to remove slag, take the supernatant alcohol precipitation (alcohol concentration 70%) 2-3 times, collect the precipitate, and get 120 g of umbellate polysaccharide.

Embodiment 3

[0155] Weigh 1Kg of mycelium of Phosphorus polyphagoides, add 20Kg of water, and extract three times at 98°C, each time for 1 hour. The extracts were combined, filtered, concentrated in vacuo under reduced pressure, and the thermal specific gravity was 1.04, and finely filtered. Add edible ethanol to precipitate, and the final concentration reaches 80%. Filter to collect the precipitate and dissolve in water. Centrifuge to remove slag, take the supernatant alcohol precipitation (alcohol concentration 80%) 2-3 times, collect the precipitate, and get 100 g of umbellate polysaccharide.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A fat-phosphorus fungus strain Pa-I.H, the process for culturing said fungus with said strain, its medicinal application and its medicinal composition are disclosed. Its culturing process includes slant culture, deep liquid fermenting, seed culture, mycelium culture and sporophore culture. Its extracts can be used for improving immunity and preventing and treating tumor.

Description

Technical field: [0001] The invention provides a new strain of Phosphorus polylipidus Pa-I·H and a method for artificially cultivating Phosphorus polylipidus with this strain, and discloses its medicinal use and a pharmaceutical composition with its active ingredients, belonging to Involved in the field of fungal artificial cultivation and medicinal technology Background technique: [0002] The fatty phosphate umbrella Pholiota adiposa (Fr.) Que is commonly known as thorn mushroom, yellow mushroom, and yellow umbrella. It belongs to Basidiomycotina, Phytomycota, Agaricaceae, Strophariaceae, and Phosphorus genus in taxonomy. Its fruiting bodies are born on the trunks of willows and birches in autumn in Jilin Province. The new bacterial strain provided by the present invention (hereinafter referred to as: Pa-I·H bacterial strain) and the application of this bacterial strain grow the fruiting body of the polylipid phosphatus umbrella through cultivation experiments, and after ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/04A61K31/715A61P35/00A61P37/04A61P29/00C12R1/645
Inventor 毛玉邦魏成松于春波
Owner SHANGHAI HI TECH UNITED BIO TECHCAL RES