DDT pesticide residue degradation bacterium and produced bacterium agent

A technology for degradation of pesticide residues and DDT, applied in the field of biological high, can solve problems such as affecting the quality and export of agricultural products, high detection rate of organochlorine pesticides, and difficulty in decomposing organochlorine pesticides, so as to solve the problem of excessive pesticide residues and protect health. , good removal effect

Inactive Publication Date: 2008-09-03
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

Organochlorine pesticides are not easy to decompose, insoluble in water, heat-resistant and acid-resistant, soluble in fat and organic solvents, so the residual time on the ground, water and objects is long, resulting in a high detection rate of organochlorine pesticides in agricultural products, which seriously affects the quality of agricultural products. quality and export

Method used

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  • DDT pesticide residue degradation bacterium and produced bacterium agent
  • DDT pesticide residue degradation bacterium and produced bacterium agent
  • DDT pesticide residue degradation bacterium and produced bacterium agent

Examples

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Comparison scheme
Effect test

Embodiment Construction

[0017] Examples of the present invention are described below.

[0018] The original seed of the microbial organophosphorus pesticide residue-degrading bacteria DB-1 of the present invention is activated on a petri dish, and its degradation performance is measured, and it is inoculated on the inclined surface of a test tube for later use. The test tube seeds were inoculated into 1000ml shake flasks containing 200ml broth medium, cultured with constant temperature shaking until logarithmic phase, and prepared to inoculate seed tanks. 500 liters of seed tanks, 400 liters of feeding capacity, the medium formula is: glucose 0.8%, (NH 1 ) 2 SO 1 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH 7.2-7.5. After the feeding is completed, 121°C high-pressure damp heat sterilization, after cooling to 33°C, inoculate the above-mentioned cultivated shake flask strains into a 500-liter seed tank according to the inoculum amount of 10%, and cultivat...

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Abstract

The invention provides DDT pesticide residue degrading bacterial and its produced bacterial agent. The used strain is gram stain reaction positive bacterial strain DB-1. And it is sphingomonas sp by identifying. Its main bionomics is G-; thallus is short baculiform and its size is 0.28 um-0.54um*0.69um-0.85umum its end has flagellum; concurrently character like oxygen; catalase and oxidase are positive; V.P. and indole reactions are negative; it can not hydrolyze starch, oxidize glucose to produce acid, and make litmus milk acid solidify. The strain 16S rDNA Genbank debarkation number is AY947554. The degrading bacterial product can reduce pesticide residue by over 90%, and produce green farm products.

Description

1. Technical field [0001] The invention relates to a DDT pesticide residue degrading bacterium and a bacterial agent produced therefrom, which belong to the high-tech field of biology, use microorganisms to degrade chemical pesticide residues, and are suitable for the production and processing of green and pollution-free agricultural products in modern agricultural production. 2. Technical background [0002] The use of chemical pesticides has greatly promoted the development of modern agriculture, restored a large number of losses caused by diseases and insect pests for agricultural production, and greatly improved the labor efficiency and mechanization degree of agricultural production, playing a role in the modernization of agricultural production. immeasurable effect. But at the same time, pesticides are some toxic and harmful chemicals. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but also cause serious harm t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/00C12R1/01
Inventor 李顺鹏张明星洪青何健郭鹏
Owner NANJING AGRICULTURAL UNIVERSITY
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