Method for preparing 3D porous bracket of chitosan ¿C copolymer of poly lactic acid

A polylactic acid copolymer, three-dimensional porous technology, applied in medical science, prosthesis, etc., to achieve the effect of eliminating the secondary molding process, low cost, and avoiding the use of organic solvents and catalysts

Inactive Publication Date: 2008-11-12
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

About making chitosan-polylactic acid copolymer three-dimensional porous scaffold, especially preparing chitosan-polylactic acid copolymer porous scaffold by in-situ molding, there is no literature report yet

Method used

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  • Method for preparing 3D porous bracket of chitosan ¿C copolymer of poly lactic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Weigh 1.5g of chitosan, dissolve it in 2.25g of DL-lactic acid, and stir for 0.5 hours until it dissolves completely. Weigh 2.5g of sodium chloride and add to this solution, stir mechanically for 3 hours, wait for uniform mixing, and heat at 50°C for 10 minutes. Pour the solution into a plastic mold, let it stand at room temperature for 24 hours, and then put it into an oven at 40° C. and dry it under normal pressure for 96 hours. In three steps, the dried and formed solids are heated and copolymerized under vacuum conditions:

[0024] ①40-50℃, 0.06MPa, heating for 1.5 hours

[0025] ②75-80℃, 0.08MPa, heating for 2-3 hours

[0026] ③85-95℃, 0.1MPa, heating for 2-3 hours

[0027] The solid matter taken out was continuously extracted with 500mL methanol for 24 hours, then placed in a vacuum drying oven, and further dried at room temperature and 0.1MPa for 48 hours to remove residual methanol. Then soak the solid matter in deionized water, change the water once every 2...

Embodiment 2

[0029] Weigh 1.5g of chitosan, dissolve it in 6g of L-lactic acid, and stir for 0.5 hours until it dissolves completely. Weigh 2.5g of sodium chloride and add to this solution, stir mechanically for 1 hour, wait for uniform mixing, and heat at 50°C for 5 minutes. Pour the solution into a plastic mold, let it stand at room temperature for 24 hours, and then put it into an oven at 30° C. and dry it under normal pressure for 96 hours. The vacuum copolymerization process is the same as above. The removed stent was continuously extracted with 500 mL of methanol for 40 hours, then placed in a vacuum drying oven, and further dried at room temperature and 0.1 MPa for 48 hours to remove residual methanol. Then soak the stent in deionized water, change the water every 24 hours, and wash it repeatedly 14 times, no sodium chloride can be detected in the deionized water after washing, and the wet stent is obtained. Wet scaffolds were freeze-dried at -50°C to prepare porous scaffolds. Th...

Embodiment 3

[0031] Weigh 1.5g of chitosan and dissolve it in 3g of lactic acid, stir for 1 hour until the dissolution is complete. Weigh 1.5g of potassium chloride and add to this solution, stir mechanically for 3 hours, wait for uniform mixing, and heat at 40°C for 10 minutes. Pour the solution into a plastic petri dish, let it stand at room temperature for 12 hours, and then put it into an oven to dry at 60°C under normal pressure for 36 hours. The vacuum copolymerization process is the same as above. The removed stent was continuously extracted with 500 mL of methanol for 40 hours, then placed in a vacuum drying oven, and further dried at room temperature and 0.08 MPa for 48 hours to remove residual methanol. Then soak the stent in deionized water, change the water once every 24 hours, and wash it repeatedly 14 times, no potassium chloride can be detected in the deionized water after washing, and a wet stent is obtained. Wet scaffolds were freeze-dried at -50°C to prepare porous scaf...

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Abstract

A 3D porous chitosan-polylactic acid scaffold is prepared through dissolving chitosan in lactic acid, adding sodium (or potassium) chloride, stirring while heating, loading it in mould, drying in oven, heating while vacuum copolymerizing, extracting in methanol or chloroform, drying, washing with deionized water, and freeze drying.

Description

technical field [0001] The invention relates to a preparation method of a three-dimensional porous scaffold of chitosan-polylactic acid copolymer, which belongs to the preparation technology of tissue engineering scaffold. Background technique [0002] The materials used for tissue engineering scaffolds mainly include natural polymer materials and synthetic polymer materials. The former generally has better cell affinity, but due to different sources of materials and different processing methods, it is difficult to reproduce product performance. The adjustable range of conventional physical and chemical properties is very limited, and the narrow operating range during processing also limits its application in special-shaped materials; synthetic polymer materials have good processing properties and can be adjusted by adjusting the composition of the product and the process parameters in the synthesis process. , so that its physical and chemical properties can be regulated. H...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/18
Inventor 姚芳莲于潇张彬姚康德
Owner TIANJIN UNIV
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