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Cell culturing rack material and its preparation

A scaffold material and cell culture technology, applied in coating and other directions, can solve problems such as unfavorable cell growth and culture, low porosity, and poor penetration of pores up and down, achieve optimal tissue compatibility, overcome poor strength, and a good growth environment. Effect

Inactive Publication Date: 2009-06-03
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The Chinese invention patent "A Porous Silk Fibroin Film and Its Preparation Method" with the publication number CN1260363A discloses a porous silk film, which uses silk as the main film-forming raw material, and adopts vacuum freeze-drying to prepare the porous silk fibroin film. The film thickness, porosity is low, and the pores are poorly connected up and down, which is not conducive to the growth and cultivation of cells.

Method used

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  • Cell culturing rack material and its preparation
  • Cell culturing rack material and its preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0026] 1. Put 0.2kg of waste mulberry silk into 6L of sodium carbonate aqueous solution with a concentration of 0.05%, boil for 0.5h, repeat the treatment three times, and remove the sericin around the silk to obtain silk fibroin, which is air-dried at room temperature;

[0027] 2. Use 1.2L of calcium chloride, water and ethanol solution with a molar ratio of 1:8:2 to dissolve the dried silk fibroin into a silk fibroin solution by heating at 78±2°C;

[0028] 3. Pour the silk fibroin solution prepared in step 2 into a cellulose dialysis bag, first dialyze with tap water, and then dialyze with deionized water to remove ethanol and calcium chloride small molecules in the solution, and then use multi-layer degreasing Filtration with gauze to obtain pure silk fibroin solution;

[0029] 4. Take 100ml of pure silk fibroin solution and pour it into an area of ​​20×20cm 2 In the polystyrene plastic tray, dry at constant temperature and humidity (25°C, RH65%) to form a film, remove the...

Embodiment 2

[0036]1. Put 0.1kg of lower leg silk into 3L of sodium carbonate aqueous solution with a concentration of 0.05%, boil for 0.5h, repeat the treatment three times, and remove the sericin around the silk to obtain silk fibroin;

[0037] 2. The dried silk fibroin obtained in step 1 is heated and dissolved into a silk fibroin solution with 0.6L of calcium chloride, water and ethanol solution with a molar ratio of 1:8:2 at 78±2°C;

[0038] 3. Pour the above silk fibroin solution into a cellulose dialysis bag, first dialyze it with tap water, and then dialyze it with deionized water to remove ethanol and calcium chloride small molecules in the solution, and then filter with multi-layer degreased gauze. Obtain pure silk fibroin solution;

[0039] 4. Take 100ml of pure silk fibroin solution and pour it into an area of ​​20×20cm 2 In a stainless steel plate, frozen at -20 °C for 8 h, and then placed in a freeze dryer for vacuum drying for 20 h to obtain a sponge-like regenerated silk f...

Embodiment 3

[0046] 1. Put 0.2kg of waste mulberry silk into 6L of sodium carbonate aqueous solution with a concentration of 0.05%, boil for 0.5h, repeat the treatment three times, and remove the sericin around the silk to obtain silk fibroin, which is air-dried at room temperature;

[0047] 2. Use 1.2L of calcium chloride, water and ethanol solution with a molar ratio of 1:8:2 to dissolve the dried silk fibroin into a silk fibroin solution by heating at 78±2°C;

[0048] 3. Pour the silk fibroin solution prepared in step 2 into a cellulose dialysis bag, first dialyze with tap water, and then dialyze with deionized water to remove ethanol and calcium chloride small molecules in the solution, and then use multi-layer degreasing Filtration with gauze to obtain pure silk fibroin solution;

[0049] 4. Take 100ml of pure silk fibroin solution and pour it into an area of ​​10×20cm 2 In the polystyrene plastic tray, dry at constant temperature and humidity (25°C, RH65%) to form a film, remove the...

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Abstract

The present invention discloses one kind of cell culturing rack material and its preparation process. The cell culturing rack material is prepared with silk as main material, and through degumming, dissolving, purifying and drying to form fimbrin; dissolving together with collagen or gelatin in the same kind of solvent; high voltage electrostatic spinning to obtain 3D netted nanometer fiber non-woven felt of fimbrin base material, collagen, gelatin, etc. and final organic alcohol treatment. The cell culturing rack material has average fiber diameter smaller than 100 nm, average pore size of 1.0-5.0 microns and porosity of 70-90%. Test shows that the nanometer fiber material has no toxicity, no irritation, no sensibilization, excellent tissue compatibility and perforated pore structure, and may be used as cell culturing rack material for repairing human body tissue.

Description

technical field [0001] The invention relates to a medical biological material and a preparation method thereof, in particular to a nanofiber non-woven composite made of a silk fibroin layer as a base material and compounded with collagen, gelatin or a mixture of collagen, gelatin and silk fibroin A felt structure cell culture scaffold material and a preparation method thereof belong to the technical field of polymer materials. Background technique [0002] Before the present invention was made, a nanofiber non-woven membrane was made of a natural or synthetic polymer by electrospinning as a cell culture carrier, such as PCL, PLA, PLGA, collagen, etc. The first three were hydrophobic, It is not conducive to the adhesion of cells in a hydrophilic biological environment; the latter is a hydrophilic natural polymer, which is conducive to the adhesion and growth of cells, but due to poor spinnability and strength, and high price, it is difficult to truly Put into use. [0003] ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/34
Inventor 张幼珠尹桂波鲍韡韡王曙东吴佳林
Owner SUZHOU UNIV
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