Biological synthesis method for dark indigo
A technology of biosynthesis and indigo, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the difficulty of eliminating public concerns about the safety of genetically modified organisms, the health hazards of chemical synthesis producers, and the low production efficiency of indigo and other problems, to achieve the effect of eliminating adverse effects, convenient storage and transportation, and low cost
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Embodiment 1
[0051] Catalytic synthesis of indigo under the normal temperature of embodiment 1, (product) in vitro secretion of indigo plant seed
[0052] 1. Synthetic indigo
[0053] 1. Preparation of indole solution
[0054] Weigh 0.06g indole solid powder (product of Beijing Chemical Reagent Company, analytically pure), dissolve it in 1ml of chloroform or N,N-dimethylformamide, and form 0.5mmol / ml indole chloroform solution or indole N, N-dimethylformamide solution, put into a 1.5ml centrifuge tube or a brown finger tube, store at room temperature, and set aside.
[0055] 2. Germination of seeds
[0056] Put 3 layers of filter paper in a Petri dish (diameter 9cm), add about 4ml of distilled water to make the filter paper fully absorb moisture, until no flowing water can be seen on the surface of the filter paper. Spread the seeds of the indigo plant Isatis indigo evenly on the filter paper of the petri dish, and cover it. Make 5 Petri dishes each time. Place the Petri dish containi...
Embodiment 2
[0059] Embodiment 2, the in vitro secretion of non-indigo monocotyledonous plant seeds Catalyzed synthesis of indigo indole at room temperature The preparation of the solution is the same as that in Example 1.
[0060] Put 3 layers of filter paper in a Petri dish (diameter 9cm), add about 4ml of distilled water to make the filter paper fully absorb moisture, until no flowing water can be seen on the surface of the filter paper. The seeds of non-indigo monocot wheat (variety: "Yangmai 158" and "Nongda 170"), rice (variety "Yuefu") and corn (see Table 1) were evenly spread on the filter paper of the petri dish respectively. On, cover. Make 5 Petri dishes of each plant seed each time. The petri dish containing the seeds was incubated at 25°C in the dark. After 1-2 days, more than 90% of the seeds germinated.
[0061] When the hypocotyl of the seed bud grows to about 2cm (1-2 days after germination), the same as in Example 1, randomly add 20 μl of indole solution (solvent is ch...
Embodiment 3
[0065] Example 3, In vitro secretion of non-indigo dicotyledonous plant seeds Catalyzed synthesis of indigo indole at room temperature The preparation of the solution is the same as in Example 1.
[0066] Put 3 layers of filter paper in a Petri dish (diameter 9cm), add about 4ml of distilled water to make the filter paper fully absorb moisture, until no flowing water can be seen on the surface of the filter paper. Spread the seeds of non-indigo dicot soybean (soybean), mung bean, cowpea, amaranth, rape, Chinese cabbage, white radish, carrot, celery, eggplant, tobacco and cockscomb (see Table 2) evenly on the Place the Petri dish on filter paper and cover. 5 Petri dishes of each plant seed each time. Incubate the Petri dish containing the seeds at room temperature in the dark.
[0067] After the culture dish containing the seeds was placed in the dark at room temperature for 1-2 days, more than 90% of the seeds germinated. When the hypocotyl of the seed bud grew to about 2cm...
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