Microorganism feed addictive for improving sow reproductive performance and its preparation method
A technology for microbial feed and reproductive performance, applied in the field of agricultural biological products, can solve the problems of no outstanding novelty in the process, and achieve the effects of increasing the number of litter and the number of live births, promoting growth, and promoting ovulation.
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Embodiment 1
[0039]The microbial feed additive for improving the reproductive performance of sows and its production method, the strains used are all obtained through screening and optimization, and a two-stage fermentation process is adopted at the same time, which includes the following steps:
[0040] 1. First stage fermentation
[0041] The first step. the method that screened Bacillus licheniformis, Lactobacillus plantarum, Lactobacillus casei, Saccharomyces cerevisiae is cultivated in the same expansion medium is optimized;
[0042] Step 2. Transfer the strains into the broth medium respectively, and after culturing at 37°C for 24 hours, select the colonies with obvious colony characteristics, strong vigor and fast growth and reproduction under the microscope to separate them by streaking to save;
[0043] Step 3. Transfer the bacterial strain into an eggplant bottle equipped with nutrient agar as a medium, cultivate it at a temperature of 37°C for 48 hours, and then put it into a r...
Embodiment 2
[0054] 1. In the first stage of fermentation
[0055] Step 4. Inoculate Bacillus licheniformis, Lactobacillus plantarum, Lactobacillus casei, and Saccharomyces cerevisiae in the steam-sterilized medium according to the ratio of 40%, 15%, 10%, and 35%, respectively, and the stirring speed is 220r / min, the culture temperature is 35°C, the ventilation rate is 1:0.6, and the fermentation time is 24 hours, the compound bacterial agent expansion culture solution is obtained;
[0056] Step 5. Inoculate the compound bacterial agent expansion culture solution in the culture medium, the ratio is: strain expansion culture solution 14%, culture medium 12%, sterile water 74%, stir and mix evenly, stirring speed 220r / min, cultivate The temperature is 35 degrees, the ventilation rate is 1:0.6, and the fermentation time is 32 hours;
[0057] Take the fermentation medium according to the following weight ratio:
[0058] Bean cake powder 3%, corn flour 5%, corn steep liquor 0.5%, yeast powde...
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