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Injectable temperature-sensitive chitosan/methyl cellulose gel and its preparation process

A technology of methyl cellulose and chitosan, which is applied in the fields of polymer chemistry and biomedicine, can solve problems such as no application significance, achieve the effects of avoiding organic chemical reagents and other harmful substances, process safety, and wide application prospects

Inactive Publication Date: 2009-08-05
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Methylcellulose is also a natural polymer material with good biocompatibility and biodegradability. It also has temperature-sensitive properties, but its phase transition temperature is between 50-70 ° C. It is used in injectable tissue engineering and There is no application significance in the drug delivery system

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment 1: 200mg chitosan is dissolved in the hydrochloric acid solution that 10ml concentration is 0.1mol / l, after dissolving completely, place 15min in ice bath; After dissolving methyl cellulose, be mixed with 2wt% solution; Add 84 mg of sodium hydrogen into the methylcellulose solution, and also place it in the ice bath for 15 minutes; then slowly drop the methylcellulose salt solution into the chitosan hydrochloric acid solution under the condition of constant stirring and ice bath, and keep the solution without turbidity , stirred until the two are fully mixed, the pH value of the chitosan and methylcellulose blend solution is 6.7, and then centrifuged to remove air bubbles. Finally, placing the blended solution at the gel temperature can form an injectable thermosensitive chitosan / methylcellulose gel, the mass percent content of chitosan in the gel is 1%, methylcellulose The mass percent content is 1%, and the gel temperature can be measured by a dynamic rheom...

Embodiment 2

[0011] Embodiment 2: 200mg chitosan is dissolved in the acetic acid solution that 10ml concentration is 0.12mol / l, after dissolving completely, place 15min in ice bath; After methylcellulose is dissolved, be mixed with 2wt% solution; Phosphoric acid Add 100 mg of sodium to the methylcellulose solution, and place it in an ice bath for 15 minutes; then slowly drop the methylcellulose salt solution into the chitosan hydrochloric acid solution under constant stirring and ice bath conditions to keep the solution free of turbidity. Stir until the two are fully mixed, the pH value of the chitosan and methylcellulose blend solution is 6.8, and then centrifuge to remove air bubbles. Finally, the blended solution is placed at the gel temperature to form an injectable thermosensitive chitosan / methylcellulose gel. The mass percentage of chitosan in the gel is 1%. The mass percent content of cellulose is 1%, and the gel temperature can be measured by a dynamic rheometer.

Embodiment 3

[0012] Embodiment 3: 100mg chitosan is dissolved in the hydrochloric acid solution that 10ml concentration is 0.05mol / l, after dissolving completely, place 15min in ice bath; After methylcellulose is dissolved, be mixed with 3wt% solution; Glycerin Add 500 mg of sodium phosphate to the methylcellulose solution, and place it in the ice bath for 15 minutes; then, under the condition of constant stirring and ice bath, slowly drop the methylcellulose salt solution into the chitosan hydrochloric acid solution to keep the solution without Turbidity, stir until the two are fully mixed, the pH value of the chitosan and methylcellulose blend solution is 6.7, and then centrifuge to remove air bubbles. Finally, the blended solution is placed at the gel temperature to form an injectable thermosensitive chitosan / methylcellulose gel. The mass percentage of chitosan in the gel is 0.5%. The mass percent content of cellulose is 1.5%, and the gel temperature can be measured by a dynamic rheomet...

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Abstract

The invention discloses an injectable thermosensitive chitosan / methylcellulose blend gel and a preparation method thereof. The gel is a physical gel, and its effective composition includes chitosan and methyl cellulose, and the weight percentages of the two are 0.25-2% and 0.25-5% respectively. The preparation method is to dissolve chitosan in an acid solution to obtain a 0.5-4wt% chitosan acid solution, and place it in an ice bath; dissolve the alkaline salt in a 0.5-10wt% methylcellulose solution, and Place in an ice bath; then slowly drop the methylcellulose salt solution into the chitosan acid solution under constant stirring to keep the solution free from turbidity, stir until the two are fully mixed, and centrifuge to remove air bubbles, then place the blended solution The gel is formed at the gel temperature. This new type of injectable temperature-sensitive gel has simple and mild preparation conditions, can avoid organic chemical reagents and other harmful substances, is suitable for the delivery of sensitive macromolecules such as protein drugs, and can also be used as an injectable medical material and tissue engineering scaffold materials.

Description

technical field [0001] The invention relates to an injectable thermosensitive chitosan / methylcellulose gel and a preparation method thereof. It belongs to the field of polymer chemistry and biomedicine. Background technique [0002] Hydrogels have a hydrophilic three-dimensional polymer network structure that can absorb large amounts of water. The physical and chemical crosslinking between polymer chains can keep the hydrogel in a certain shape and not dissolve in water. Due to their good biocompatibility, hydrogels have been widely used in the field of biomedicine. Among them, the in situ formation of gel system is a research hotspot, which has a variety of biological applications, mainly used in drug sustained release, cell culture and tissue engineering. The in-situ gel-forming system remains in a solution state before injection, and can form a gel at body temperature after injection. Factors such as solvents, UV radiation, ionic crosslinkers, pH, and temperature can ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/36A61L27/20
Inventor 杜予民汤玉峰李艳
Owner WUHAN UNIV
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