Fowl multi-killed pasteurellosis bacillus live vaccine and preparation method thereof

A Pasteurella, multocida technology, applied in the direction of pharmaceutical formulations, local antibacterial agents, medical preparations containing active ingredients, etc., can solve problems such as inappropriate oral administration, food contamination, muscle necrosis, etc., to improve immunity, The effect of extending the protection period and low cost

Inactive Publication Date: 2009-11-04
JIANGSU INST OF POULTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the vaccine products used in my country to prevent fowl cholera include "fowl cholera propolis inactivated vaccine", "fowl cholera aluminum hydroxide inactivated vaccine", "fowl cholera oil emulsion inactivated vaccine" and "pasteurella multocida G 190 However, these vaccines have two disadvantages: one is that both inactivated vaccines and live vaccines need to be immunized by injection, which will cause various stress reactions (injection local inflammation, muscle necrosis, food loss and Second, because some drug residues in the inactivated vaccine will cause food pollution, it can neither be used for fast-growing meat poultry or poultry that is ready to go on the market within 6 weeks, nor is it suitable for oral administration

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Pasteurella multocida P 7810 After rejuvenation of freeze-dried strains, typical bacterial colonies are selected and cultured to make seed bacterial liquid, and then the seed bacterial liquid is cultured, multiplied and concentrated, then added with 1.5% gelatin and 5% sucrose, and then packaged and freeze-dried. A total of 45 healthy Laihang chickens in three groups of 140 days were immunized with the vaccine through the drinking water route. The test chickens in each group drank the vaccine at the same time, and the challenge time was the third day, the fifth day and the third day after immunization respectively. Day 8. Results After immunization, the protection rate of each group was 70-80% on the 3rd day; 75-95% on the 5th day; 80-100% on the 8th day. From this, it can be judged that Pasteurella avium multocida P 7810 The live vaccine can produce better immune protection on the 3rd day after immunization, and can produce strong protection on the 8th day.

Embodiment 2

[0015] The vaccine made by the preparation method described in Example 1 carried out oral immunization to three groups of 150-day-old healthy Laihang chickens respectively, and 14 days after the immunization, respectively used three different serotypes of Pasteurella multocida virulent strain C 48-1 (A:5), P 1059 (A:8) and P 2723 (A: 9) attacks, and the result shows: A: three batches of protection rates of 8 type strong virus strain attacks are 60%, 40% and 60%; A: the protection rate of 9 type strong virus strain attacks is 60%; Isotype virulent strain C 48-1 Attacks have protection rates of 100%, 80%, and 100%. Judging from this, P 7810 The oral vaccine not only has a strong protective force against the attack of the same type of strong virus strain, but also has a protection rate of 40%-60% against the attack of the heterotype of strong virus strain.

Embodiment 3

[0017] The vaccine made by the preparation method described in Example 1 carried out drinking water immunization to 375 healthy chickens of five combinations respectively, and then every month with getting a certain number of test chickens to carry out strong poisonous nasal drip challenge; test result shows: oral vaccine 5 The protection rates of each group during the one-month immunization period were 77.8%, 80%, 80%, 80%, 80%, with an average of 79.6%; the protection rates of each group during the 6-month immunization period were 69.2%, 76.9%, 66.7% respectively. %, 80%, 90%, with an average of 75.4%. According to the above data, the P 7810 The immunization period of the oral vaccine is set at 6 months to ensure that the average protection level of the population reaches more than 75%.

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Abstract

The present invention discloses one kind of live vaccine of fowl Pasteur's multocidum bacterium and its preparation process, and belongs to the field of biological product technology. The live vaccine is prepared with natural low virulent strain P7810 of fowl Pasteur's multocidum bacterium, and through steps of anabiosis and passage of the bacterium seed to form seed bacterium liquid, culturing to proliferate, adding gelatin in 1.5 % and cane sugar in 5 %, packing and freeze drying to obtain live vaccine of fowl Pasteur's multocidum bacterium P7810. The live vaccine is fed to fowl for immunizing to prevent fowl cholera, and has immunizing period as long as 6 months, high protecting rate, low cost and other advantages.

Description

technical field [0001] The invention relates to a Pasteurella multocida live vaccine used for oral immunization of poultry and a preparation method of the vaccine, belonging to the technical field of biological products. Background technique [0002] Pasteurella multocida (Pasteurella multocida P.m) is a contact septic infectious disease that causes fowl cholera in poultry and wild fowl, with high morbidity and mortality. Infected birds can die within 48 hours of 90% -100%. At present, in my country's poultry infectious diseases, especially waterfowl (ducks and geese), the economic losses caused by fowl cholera are still very serious. According to poultry epidemic statistics, the number of poultry deaths due to fowl cholera epidemic accounts for 1.3% of the total annual poultry breeding in my country and 1.9% of the total water fowl breeding. At present, the vaccine products used in my country to prevent fowl cholera include "fowl cholera propolis inactivated vaccine", "fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/102A61P31/02
Inventor 刘学贤吕晓娟徐步龚建森
Owner JIANGSU INST OF POULTRY SCI
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