Isolation and expression of a gene for a nitrilase from acidovorax facilis 72W

A nitrilase and chimeric gene technology, applied in the field of molecular biology, can solve the problems of lack of thermostable high-yield nitrilase, thermal instability limiting industrial application and the like
CN100558885CInactive Publication Date: 2009-11-11THE CHEMOURS CO FC LLC
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
THE CHEMOURS CO FC LLC
Publication Date
2009-11-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

Recombinant microbial strains expressing nitrilase enzymes and useful as biocatalysts for hydrolyzing nitrile-containing substrates are provided. The recombinant cells were transformed with an exogenous gene isolated from Acidovorax facilis 72W encoding a thermostable nitrilase that catalyzes the hydrolysis of nitrile-containing substrates to carboxylic acids under mild reaction conditions. The nucleotide sequence of the nitrilase gene and the putative amino acid sequence encoded by the nitrilase gene are provided.
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Description

field of invention

[0001] The invention relates to the field of molecular biology and the use of recombinant microorganisms to express target genes and gene products. More specifically, the gene used in the present invention is a novel nucleic acid fragment encoding a nitrilase that catalyzes the hydrolysis of a wide variety of nitrile-containing substances to produce the corresponding carboxylic acids. The present invention also provides recombinant strains expressing nitrilase activity and useful as biocatalysts for the hydrolysis of nitrile-containing substrates. In addition, the present invention relates to specific nucleic acids that facilitate the isolation of said nitrilase gene. Background of the invention

[0002] Nitriles are readily converted to the corresponding carboxylic acids by various chemical methods, but these methods usually require strongly acidic or basic reaction conditions and high reaction temperatures, and often produce unwanted by-products and / or ...

Claims

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