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Compositions containing bioactive materials, methods of preparation and treatment

A technology for biologically active substances and compositions, which can be applied to medical preparations containing active ingredients, drug combinations, organic active ingredients, etc., can solve the problems of limited selection of antibodies, difficulties in logistics and production costs, difficulties in regulation and food safety, etc.

Inactive Publication Date: 2009-12-23
IMMURON
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Low concentrations of specific antibodies severely limit therapy options
[0027] Furthermore, cows immunized to produce colostrum also produce milk for human consumption, which poses problems of regulation and food safety
[0028] In addition, colostrum can only be harvested once a year from cows at calving, which poses logistical and costly difficulties, especially for bioactive components secreted by cows into colostrum

Method used

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  • Compositions containing bioactive materials, methods of preparation and treatment
  • Compositions containing bioactive materials, methods of preparation and treatment
  • Compositions containing bioactive materials, methods of preparation and treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Example 1 Activity of colostrum-protected enzymes in the gastric environment as an example of bioactive protein protection

[0084] introduce:

[0085] The role of bovine colostrum as a protector of bioactive protein activity in the gastric environment was studied using urease. To investigate the protective properties of colostrum, the effect of simulated gastric juice on urease activity was compared in the presence and absence of bovine colostrum.

[0086] Materials and methods:

[0087] Reagent

[0088] Urease: Type III canavalan urease (Cat No U-1500) from Sigma Company was used as the source of the enzyme. Lyophilized urease (labeled enzyme activity is 16u / mg; at pH 7.0 and 25°C, 1u releases 1.0μmol of ammonia from urea per minute) was dissolved in MilliQ water to a concentration of 0.3u / μl (about 21mg / ml), and kept frozen for future use.

[0089] Simulated gastric juice (SGF): (modified according to the method of Hilger et al. 2001). A 0.32% porcine pepsin...

Embodiment 2

[0113] Example 2 : Process for preparing bovine colostrum powder

[0114] The flow chart below shows the principle of obtaining colostrum and converting it into a processed form.

[0115]

[0116] The most preferred raw colostrum is colostrum harvested from dairy cows after calving for the first milking. Colostrum is stored at 4°C on the farm, and then stored at -20°C for long-distance transport or directly sent to milk processing.

[0117] The crude colostrum is heated to about 37°C and then the fat is removed using a rotary milk skimmer. The resulting liquid is pasteurized or microfiltered (to remove bacteria and debris) with a 7-10 micron ceramic filter system. The liquid is then subjected to ultrafiltration (e.g. using an Abcor 10m 2 Ultrafiltration equipment) to remove most of the water, lactose and electrolytes, leaving a high protein concentrate. The resulting high protein concentrate is further processed, preferably freeze-dried (freeze-dried) or spray-dried. ...

Embodiment 3

[0138] Example 3 : Protection of colostrum against antibody activity during acid / pepsin treatment

[0139] The effect of bovine colostrum as a protective agent on protein activity in the gastric environment was studied using an antibody model. To investigate the protective properties of colostrum, the effect of simulated gastric juice on the biological activity of influenza virus-specific monoclonal antibodies was compared in the presence and absence of bovine colostrum.

[0140] Materials and methods:

[0141] Reagent:

[0142] Influenza virus and specific monoclonal antibody: The influenza A virus used in this study was Mem71 H -Bel N (H3N3). The antibody used was Mem71 H -Bel N Specific IgG2a monoclonal (Mab 36 / 2), diluted 1:10 in phosphate buffered saline.

[0143] Simulated gastric juice (SGF): (modified according to the method of Hilger et al. in 2001) prepare 0.32% of Sigma's porcine pepsin (Cat No P-7012 derived from gastric mucosa) solution in 0.03M NaCl solu...

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Abstract

The present invention relates to a method of increasing the viability of an unstable biologically active substance administered in an adverse environment, the method comprising forming a mixture of the biologically active substance and mammalian colostrum, also comprising a drug for the unstable biologically active substance A composition comprising a mixture of a biologically active substance and mammalian colostrum is used.

Description

technical field [0001] The present invention relates to compositions containing biologically active substances and methods of their use. Background technique [0002] Bioactive substances, which may have strong physiological effects, are often very sensitive to adverse environments, such as the environment of the mammalian stomach, high temperature environment or high humidity environment. This tends to limit their use, meaning they are not convenient for oral administration, storage or implementation on warm production lines. [0003] The biologically active substance may be selected from the group consisting of growth promoters, antineoplastic agents, oral vaccines, inhalants, live microorganisms (e.g. protozoa such as lactic acid bacteria), peptides, polypeptides, nucleotides, polynucleosides acids, nucleosides, proteins, glycoproteins, sugar and carbohydrate complexes, anti-infective agents, antibacterial agents, disinfectants, bactericides, antidepressants, psychoactiv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/20A61K38/00A61K31/713A61K39/00A61K39/02A61K35/74A61K39/40A61K39/42A61P31/00A61P1/12A61K35/745A61K35/747A61K47/46
CPCA61K35/747A61K35/20A61K47/46A61K35/745A61K45/06A61P1/12A61P31/00A61K2300/00A61K31/713A61K35/74A61K39/395
Inventor 格兰特·托马斯·罗林戈特弗里德·利希蒂罗伊·迈克尔·罗宾斯-布朗
Owner IMMURON