Method for synthesizing cation plant fibre strengthening agent by modified glutelin protein
A technology of gluten protein and plant fiber, applied in the field of papermaking, can solve the problems of reducing the molecular weight of protein, can not be used to strengthen the fiber strength, can not be used as a fiber reinforcing agent, etc., and achieves high conversion efficiency, mild reaction conditions, and enhanced wet strength. Effect
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Embodiment 1
[0016] First, 10 grams of gluten protein was dissolved in 100 mL of 0.2% KCl and pH=8 buffer solution, and 0.024 moles of substituted diamino compound H was added. 2 N(CH 2 ) n NH(CH 2 ) m CH 3 (n=2, m=0), stirred evenly, then added 0.6 g of transglutaminase, and reacted at 50° C. for 1 hour to obtain grafted protein.
[0017] Then, in the grafted protein solution, drip epichlorohydrin (mass ratio is 1: 1), continue in 60 ℃ water bath, react 1 hour, obtain modified gluten protein synthesis cationic plant fiber reinforcing agent, pH value is 7.5. The product is stable, and there is no precipitation after 30 days.
Embodiment 2
[0019] First, 10 grams of gluten protein was dissolved in 100 mL of 0.2% KCl and pH=8 buffer solution, and 0.024 moles of substituted diamino compound H was added. 2 N(CH 2 ) n NH(CH 2 ) m CH 3 (n=6, m=1), stir evenly, then add 0.6 g of transglutaminase, react at 30° C. for 4 hours, and obtain grafted protein.
[0020] Then, in the grafted protein solution, drip epichlorohydrin (mass ratio is 1: 1), continue in 30 ℃ of water baths, react for 4 hours, obtain modified gluten protein synthesis cationic plant fiber reinforcing agent, pH value is 7.2.
Embodiment 3
[0022] First, 5 grams of gluten protein was dissolved in 50 mL of 0.2% KCl and pH 9 buffer solution, and 0.017 moles of substituted diamino compound H was added. 2 N(CH 2 ) n NH(CH 2 ) m CH 3 (n=2, m=0), stir evenly, then add 0.3 g of transglutaminase, react at 30° C. for 2 hours, and obtain grafted protein.
[0023] Then, in the grafted protein solution, add epichlorohydrin (mass ratio is 1: 1) dropwise again, continue to react in 50 ℃ water bath for 2 hours, obtain modified gluten protein synthesis cationic plant fiber reinforcing agent, pH value is 7.5.
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