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Deoxynivalenol detection method based on single chain antibody, and kit

A technology of deoxynivalenol and single-chain antibody, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long time consumption, complicated production process, high cost, etc., and achieve easy production and simple manufacturing process , the effect of reducing costs

Inactive Publication Date: 2011-08-24
FUJIAN AGRI & FORESTRY UNIV +1
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Problems solved by technology

The whole production process is complicated, takes a long time and costs high, especially it requires skilled professional technicians to be competent

Method used

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  • Deoxynivalenol detection method based on single chain antibody, and kit
  • Deoxynivalenol detection method based on single chain antibody, and kit
  • Deoxynivalenol detection method based on single chain antibody, and kit

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Embodiment Construction

[0034] The specific detection steps of the method are:

[0035] 1) Coating: Dilute the antibody against a tag consisting of 13 amino acids, that is, the antibody against E-tag, in the coating buffer to a final concentration of 15 μg / mL, and add it to the polymer with a multichannel pipette. Add 100 μL / well to the microwells of the styrene ELISA plate, and coat overnight in a refrigerator at a temperature of 4°C;

[0036] 2) Washing: Discard all the liquid in the microwells of the coated ELISA plate, and wash 3 times with phosphate buffer solution. The washing method is to first add 300 μL of washing solution to each microwell, and after standing for 3 minutes, put Pour out all the washing solution in the microwell, then add the same volume of new washing solution, repeat the washing operation, a total of 3 times;

[0037] 3) Blocking: add 300 μL of blocking solution to each microwell, and block in an incubator at 37° C. for 1 hour;

[0038] 4) Washing: Discard all the liquid...

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Abstract

A method for detecting deoxy snow mold fusarine enol based on single chain antibody includes using separated single chain antibody of snow mold fusarine enol to detect relevant toxin deoxy snow mold fusarine enol and applying competitive enzyme-linked immunosorbent analysis to detect deoxy snow mold fusarine enol content in detected sample. The kit used for realizing said method is also disclosed.

Description

technical field [0001] The present invention relates to a rapid detection method of mycotoxins, more specifically to a single-chain antibody-based detection method for deoxynivalenol and a kit thereof. Background technique [0002] Head blight caused by Fusarium is an important disease that harms cereal crops such as wheat, barley, oats, rye, and corn, especially in warm and humid areas. Fusarium scab not only reduces crop yield, reduces grain quality and edible value, but also produces a variety of toxins that can poison humans and animals, posing a serious threat to human and animal health. Current toxin detection methods include thin-layer chromatography, gas-liquid chromatography, mass spectrometry, nuclear magnetic resonance, and immunochemistry. Enzyme-linked immunosorbent assay (ELISA) technology is a new immunochemical technology developed rapidly in recent years. It has been widely used in the detection of trace poisons. Enzyme-linked Immunoadsorbent Assay (ELISA ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N33/545
Inventor 王宗华汪世华鲁国东陈涵杜晓煜黄雨墨
Owner FUJIAN AGRI & FORESTRY UNIV
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