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Scanning tip and process for its production and use, particularly for a scanning probe microscope

InactiveUS6767696B2Producing scanning tips and similar devicesEasy and economical to produceNanotechPhotomechanical apparatusResistImage resolution
The invention relates to so-called scanning tips of probes necessary for scanning a measured object, in particular in scanning force microscopes and other scanning microscopes, so-called scanning probe microscopes. The possible resolution of such microscopes depends primarily on the fineness of the tip, i.e. its curvature or radius being as small as possible. According to the invention, a photostructurable material, e.g. a photosensitive resists, serves as the material for the scanning tip which is exposed via a mask and is subsequently developed / hardened in a manner known per se. The unexposed parts of the photosensitive resist are removed as usual. By the shape of the exposure mask, the preferably directed exposure of the photosensitive resist, and the subsequent hardening, a tip is formed preferably laterally on or at a carrier, usually made from a different material, which is provided with a very small radius, thus very well suitable for scanning probe microscopy and similar applications. By an appropriate selection of the mask and / or the exposure angle, the geometry of the tip can be controlled, in particular its bending radius and edge angle, for other applications as well, e.g. for measuring scanners or profile meters.
Owner:NANOSURF +1

Gene engineering antibody for kitasamycin residue detection, and preparation method and application thereof

The invention discloses a gene engineering antibody for kitasamycin residue detection, and a preparation method and application thereof. The preparation method comprises the following steps: A. taking hybridoma cell strain KA/2A9 preserved in liquid nitrogen, extracting splenocyte total RNA (ribonucleic acid) to obtain mRNA (messenger ribonucleic acid), and carrying out reverse transcription to obtain cDNA (complementary deoxyribonucleic acid); B. amplifying to obtain heavy chain variable region and light chain variable region genes; by using heavy chain and light chain antibody segments as primers, connecting the antibody heavy chain with the light chain by using a polypeptide chain; C. connecting digestion scFv into E.coliTG1, adding M13K07, and culturing to obtain a bacteriophage antibody library of kitasamycin-specific antibodies; D. adding the bacteriophage into E.coliHB2151 in a logarithmic growth phase, and carrying out IPTG (isopropyl-beta-D-thiogalactopyranoside) induction to obtain a specific bacteriophage antibody; and E. sequencing the verified Escherichia coli expression strain to obtain the single chain antibody amino acid and nucleotide sequences. The method is easy to implement, simple to operate and convenient for mass production of gene engineering antibodies. The invention is widely used for detecting kitasamycin residues in animal edible tissues, and provides residue level information of kitasamycin in animal edible tissues.
Owner:HUAZHONG AGRICULTURAL UNIVERSITY
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