Process for producing high-purity kanjak mannan-oligosaccharides

Abstract

The invention provides a method for preparing high- purity konjak mannan with production purity over 95%. It comprises following steps: taking konjaku flour as raw material, producing mannan through enzymolysis, centrifuging and filtering and microfiltering and purifying, desalting with ion-exchange technology, decoloring, odour- removing, cutting off macromolecule with ultrafiltering membrane technology from sugar liquid, spray drying and getting high purity konjak mannan with purity over 95%. The invention is characterize by employment of enzyme technology, ion- exchange technology, membrane technology system, simple operation, good separation effect, low cost, no chemical pollution, realization of industrial production. The product can not only be used as functional health care product, but aslo as high- grade food ingredient, medical intermediate, and high- grade feed addictive.

Description

technical field [0001] The invention relates to the production and preparation of oligosaccharides in the food industry, especially the production method of high-purity mannan oligosaccharides. Background technique [0002] Oligosaccharides (oligosaccharides, Oligosaccharides) refer to a class of sugars in which 2 to 10 monosaccharide units are linked by glycosidic bonds to form straight or branched chains. In addition to good physical and chemical properties such as low heat, stability, safety and non-toxicity, these sugars also have the physiological function of increasing beneficial bacteria and reducing harmful bacteria in the intestine. [0003] High-purity mannan oligosaccharides are the best among oligosaccharides, with great potential for development. The main raw material for the production of manna oligosaccharides is konjac fine powder, and the main active ingredient of konjac fine powder is konjac glucomannan (Gluvomannan, referred to as KGM), which accounts for...

AI Technical Summary

Problems solved by technology

[0006] At present, in countries such as Europe and the United States, due to limited resources, chemical synthesis methods and separation and extraction of mannan oligosaccharides from yeast cell walls are mainly used, and the product performance is poor.

In my country, the Institute of Microbiology of the Chinese Academy of Sciences has cooperated with Yunnan to carry out tests on the application of alkaline bacteria. The production process is complicated and the cost is very high. Bulletin, 1995, 22 (3): 154-157) studies the production of mannanase by Bacillus licheniformis fermentation, Li Jianfang et al. β-mannanase, but these studies are only at the laboratory stage, the enzyme activity is not high, staying at 300-500μ / ml, the conversion rate of mannan is low, only 20-80%; the purity of mannan oligosaccharides Low, only 30-80%; the production process is complicated, the cost is high, and industrialization cannot be realized