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Short peptide for inhibiting Shiga toxin and application thereof

A Shiga toxin and short peptide technology, applied in the direction of peptides, anti-toxins, peptide/protein components, etc., to achieve good application prospects, excellent binding kinetics, and the effect of inhibiting toxicity

Active Publication Date: 2007-08-15
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Peptide inhibitors against Shiga toxins have not been reported so far

Method used

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  • Short peptide for inhibiting Shiga toxin and application thereof
  • Short peptide for inhibiting Shiga toxin and application thereof
  • Short peptide for inhibiting Shiga toxin and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Recombinant Escherichia coli pBV220-stxb / DH5α

[0022] Construction and expression and identification of recombinant StxB

[0023] 1. Materials

[0024] 1.1 Strains, plasmids and media

[0025] Type I Shigella dysenteriae 51054 was purchased from China National Institute of Pharmaceutical and Biological Products for the capture of stxb gene; Escherichia coli DH5α was purchased from Dopp Company for gene cloning strain construction and exogenous protein expression; temperature-regulated high-efficiency expression Plasmid pBV220 was purchased from Takara Company and used to express foreign proteins in Escherichia coli DH5α. The pMD18-T vector was purchased from Takara Company and was used for cloning the target gene. LB-Amp medium (1% tryptone, 0.5% yeast extract, 1% sodium chloride, pH 7.0, 50 μg / mL ampicillin) was used for the cultivation of Escherichia coli and the expression of foreign proteins.

[0026] 1.2 Oligonucleotide primers

[0027]According to ...

Embodiment 2

[0047] Example 2 Chemical Synthesis and Activity Identification of Short Peptides with Shiga Toxin Toxicity Inhibitory Effect

[0048] 1. Materials

[0049] Protparam tool software (http: / / www.expasy.ch / tools / protparam.html); Short peptide of the present invention is synthesized by Institute of Bioengineering, Academy of Military Medical Sciences, BIAcore 3000 biosensor system is the product of Pharmacia Company, Balb / C small The mice were provided by the Animal Center of the Academy of Military Medical Sciences, the strain S.dys 51054 was purchased from the China Institute for the Control of Biological Pharmaceutical Products, and the StxB antibody was purchased from Biodesign (ELISA titer 1:200).

[0050] 2. Methods and Results

[0051] 2.1 Prediction of physicochemical properties of short peptide A6

[0052] The physicochemical properties of toxin-binding short peptide A6 were predicted and analyzed by protparam tool software. It shows that the molecular weight of short ...

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PUM

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Abstract

The invention discloses a making method of short peptide to inhibit shigellosis with amino acid as list 1, which is characterized by the following: adding chemical group, amino acid, peptide, protein, PEG, marked material to N and C ends to do kinds of chemical or biological decoration; optimizing the stability or other applying property and using scale; fitting for treat shigellosis, enterorrhagia escherichia coli O157 and cholera vibrio.

Description

technical field [0001] The present invention relates to a biological toxin inhibitor, in particular to a short peptide capable of inhibiting Shiga toxin, and also to a preparation method and application of the short peptide. Background technique [0002] Shiga toxin (Stx) is a kind of bacterial exotoxin with enterotoxicity, cytotoxicity and neurotoxicity produced by enteropathogenic bacteria, and it is the most common cause of emerging enteropathogens such as acute bacillary dysentery, cholera O139, O157:H7, etc. A key potent virulence factor. Clinically, it causes serious complications such as hemorrhagic colitis (hemorrhagic colitis, HC), thrombotic thrombocytopenic purpura (TTP), and hemolytic uremic syndrome (HUS) with a mortality rate as high as 80-90%. Such diseases kill millions of people worldwide. [0003] At present, there are no specific and effective preventive products and emergency treatment drugs for Shiga toxin. Antibiotic treatment commonly used in clinic...

Claims

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Application Information

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IPC IPC(8): C07K7/08A61K38/10A61P39/00
CPCY02A50/30
Inventor 王慧包士中荫俊史晶侯晓军蔡昆
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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