Fly maggot oil capable of resisting AIDS, preparing method and use for fatty acid thereof

An anti-AIDS and fatty acid technology, applied in the field of bioengineering, can solve the problems of general functions and few development of health care products, and achieve the effect of easy absorption, good inhibition and therapeutic effect, and stable quality

Inactive Publication Date: 2007-09-12
沈荣法 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fatty acids produced from vegetable oils have general functions and have a certain health care effect on the

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The fly maggots cultivated by the above breeding method are used as raw materials, and the fly maggots are washed with water, dried at a temperature of 42° C. until the water content in the fly maggots is 10% under a vacuum degree of 0.01Mpa, pulverized, and added petroleum ether of 1 times its weight, and dried at 5 Extract at ℃ for 4 hours, separate the solid and liquid phases, and evaporate the liquid phase at 30 ℃ below the vacuum of 0.01Mpa to recover petroleum ether to obtain fly maggot oil; In the buffer solution, add porcine pancreatic neutral lipase to reach 180 international units of enzyme activity per milliliter; add fly maggot oil to the replacement solution equipped with porcine pancreatic neutral lipase, and the ratio of buffer solution to fly maggot oil is 1:0.8 , kept at 38°C, stirred and reacted for 2.0 hours, stopped the reaction, separated statically, separated the oil phase, washed 3 times with an equal volume of water, and rotated the oil phase to d...

Embodiment 2

[0016] The fly maggots cultivated by the above breeding method are used as raw materials, and the fly maggots are washed with water, dried at a vacuum degree of 0.003Mpa and at a temperature of 35° C. until the water content in the fly maggots is 13%, pulverized, and 10 times its weight of ethane is added. Extract at ℃ for 2 hours, separate the solid and liquid two phases, and evaporate the liquid phase at 35 ℃ below the vacuum of 0.003Mpa to recover ethane to obtain fly maggot oil; in the buffer solution of sodium monohydrogen phosphate and sodium dihydrogen phosphate with water In, add yeast neutral lipase, reach 200 international units of enzyme activity per milliliter; Add fly maggot oil in the buffer solution that is equipped with yeast neutral lipase, the volume ratio of buffer solution and fly maggot oil is 1: 1.0, keep 40°C, stirred and reacted for 3.5 hours, stopped the reaction, statically separated, separated the oil phase, washed 4 times with an equal volume of wate...

Embodiment 3

[0018] The fly maggots cultivated according to the above breeding method are used as raw materials, and the fly maggots are washed with water, and dried at a temperature of 38°C under a vacuum of 0.008Mpa until the water content in the fly maggots is 15%, crushed, and 7 times the weight of petroleum ether and ethane are added. The mixed solvent of petroleum ether and ethane is 1:1, leaching at 20°C for 3 hours, separating the solid and liquid phases, and the liquid phase is evaporated at 40°C under a vacuum of 0.008Mpa to recover the mixture of petroleum ether and ethane Solvent to obtain fly maggot oil; in the pH7.0 buffer solution of sodium monohydrogen phosphate and sodium dihydrogen phosphate configured with water, add bacterial neutral lipase to reach 195 international units of enzyme activity per milliliter; Add fly maggot oil to the buffer solution of lipase, the volume ratio of buffer solution and fly maggot oil is 1:1.3, keep at 39°C, stir and react for 3 hours, stop t...

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PUM

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Abstract

This invention discloses a preparing method and usage of the anti-AIDS grease of housefly larva and its fatty acid. It cleans larvae in water, dries them until the content of water is below 16% when the vacuum is below 0.01 Mpa, crushes them, adds organic solvent for extraction, separates the solid and liquid phase, and recycles the solvent by evaporation to get larvae. It adds neutral lipase into the watered buffer solution of sodium mono-hydrogen phosphate and sodium dihydrogen phosphate with pH6.0~8.0, adds the neutral lypase to 180 to 200 activity international units per ml. Then it adds the grease of larva into the buffer with their volume ratio of 1:0.8~1.3, stirring until stopping the reaction, separates after rest for the oil phase, then washes with the same volume of water, evaporates the oil phase to get the lava fatty acid.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a preparation method of anti-AIDS fly maggot oil and fatty acid thereof. Background technique [0002] At present, fly maggots are used as raw materials for high-protein feed, and fly maggots are not extracted from fly maggot oil, and fly maggots are dried and powdered to be sold as feed. The production technology of fatty acid is first to use marine fish oil as raw material, hydrolyze, and then separate and purify to obtain mixed fatty acid, wherein the content of unsaturated fatty acid is relatively high. Another technique is to use vegetable oil as raw material, undergo hydrolysis, and then separate and purify to obtain mixed fatty acids. The fatty acids produced from marine fish oil, because of their high content of unsaturated fatty acids, have the functions of softening blood vessels, enhancing blood supply, and promoting brain development. Therefore, th...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12P7/40A61K35/64A61P31/18
Inventor 沈荣法陈炳香周红妹
Owner 沈荣法
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