Fly maggot oil capable of resisting AIDS, preparing method and use for fatty acid thereof
An anti-AIDS and fatty acid technology, applied in the field of bioengineering, can solve the problems of general functions and few development of health care products, and achieve the effect of easy absorption, good inhibition and therapeutic effect, and stable quality
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Embodiment 1
[0014] The fly maggots cultivated by the above breeding method are used as raw materials, and the fly maggots are washed with water, dried at a temperature of 42° C. until the water content in the fly maggots is 10% under a vacuum degree of 0.01Mpa, pulverized, and added petroleum ether of 1 times its weight, and dried at 5 Extract at ℃ for 4 hours, separate the solid and liquid phases, and evaporate the liquid phase at 30 ℃ below the vacuum of 0.01Mpa to recover petroleum ether to obtain fly maggot oil; In the buffer solution, add porcine pancreatic neutral lipase to reach 180 international units of enzyme activity per milliliter; add fly maggot oil to the replacement solution equipped with porcine pancreatic neutral lipase, and the ratio of buffer solution to fly maggot oil is 1:0.8 , kept at 38°C, stirred and reacted for 2.0 hours, stopped the reaction, separated statically, separated the oil phase, washed 3 times with an equal volume of water, and rotated the oil phase to d...
Embodiment 2
[0016] The fly maggots cultivated by the above breeding method are used as raw materials, and the fly maggots are washed with water, dried at a vacuum degree of 0.003Mpa and at a temperature of 35° C. until the water content in the fly maggots is 13%, pulverized, and 10 times its weight of ethane is added. Extract at ℃ for 2 hours, separate the solid and liquid two phases, and evaporate the liquid phase at 35 ℃ below the vacuum of 0.003Mpa to recover ethane to obtain fly maggot oil; in the buffer solution of sodium monohydrogen phosphate and sodium dihydrogen phosphate with water In, add yeast neutral lipase, reach 200 international units of enzyme activity per milliliter; Add fly maggot oil in the buffer solution that is equipped with yeast neutral lipase, the volume ratio of buffer solution and fly maggot oil is 1: 1.0, keep 40°C, stirred and reacted for 3.5 hours, stopped the reaction, statically separated, separated the oil phase, washed 4 times with an equal volume of wate...
Embodiment 3
[0018] The fly maggots cultivated according to the above breeding method are used as raw materials, and the fly maggots are washed with water, and dried at a temperature of 38°C under a vacuum of 0.008Mpa until the water content in the fly maggots is 15%, crushed, and 7 times the weight of petroleum ether and ethane are added. The mixed solvent of petroleum ether and ethane is 1:1, leaching at 20°C for 3 hours, separating the solid and liquid phases, and the liquid phase is evaporated at 40°C under a vacuum of 0.008Mpa to recover the mixture of petroleum ether and ethane Solvent to obtain fly maggot oil; in the pH7.0 buffer solution of sodium monohydrogen phosphate and sodium dihydrogen phosphate configured with water, add bacterial neutral lipase to reach 195 international units of enzyme activity per milliliter; Add fly maggot oil to the buffer solution of lipase, the volume ratio of buffer solution and fly maggot oil is 1:1.3, keep at 39°C, stir and react for 3 hours, stop t...
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