Application of fucoidan in the preparation of medicine and health-care products for preventing and treating neurodegenerative diseases

A fucoidan sulfate, neurodegenerative technology, used in nervous system diseases, neuromuscular system diseases, applications, etc.

Inactive Publication Date: 2007-09-26
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Preparation of fucoidan sulfate with fresh sea cucumber as raw material.

[0027] Fresh sea cucumber, gutted, washed, broken into 1cm pieces 3 The left and right pieces are homogenized by adding 5-15 times of water.

[0028] The homogenate was subjected to pepsin (the amount of enzyme was 0.4% of the wet weight of sea cucumber, pH 2.0-2.5, temperature 50°C, time 5h), trypsin (the amount of enzyme was 0.45% of the wet weight of sea cucumber, pH 8.0-8.5, Temperature 50°C, time 5h) enzymolysis, after enzymolysis, boiling to inactivate the enzyme activity, centrifugation (10000r / min, 10min) to take the supernatant.

[0029] The supernatant was precipitated with alcohol, centrifuged (6000r / min, 15min) to obtain the precipitate, then dissolved in distilled water, separated through a DEAE cellulose column, and the absorption peak was detected at 210nm, and the active component was collected (using the activity of nerve cells as an index), and lyophilized.

[0030...

Embodiment 2

[0032] Embodiment 2: Preparation of fucoidan sulfate with kelp as raw material

[0033] Lightly dried kelp is washed, dried and pulverized. Weigh a certain weight of powder (50g), add 5 times the volume (250ml) of absolute ethanol to reflux and extract twice, each time for 2 hours, after centrifugation, place the precipitate on a watch glass to evaporate the ethanol.

[0034] Use 5 times volume of acetone (250ml) to reflux extract the kelp powder twice, each time for 2 hours, centrifuge to get the precipitate and evaporate the acetone to dryness. Add 40 times of water (2000ml) and extract in a 70°C water bath for 5 hours, and filter with gauze to obtain the extract. Add 20 times of hydrochloric acid, pH = 6, and extract at 60°C for 6 hours. Concentrate on a rotary evaporator. Precipitate in 30% ethanol overnight, and centrifuge to retain the supernatant. Precipitate overnight in 60% ethanol: centrifuge to retain the precipitate. Freeze-drying to obtain crude fucoidan. Et...

Embodiment 3

[0035] Example 3: Dedifferentiation of neural precursor cells by fucoidan sulfate.

[0036] Wistar rats at 13.5-14 days pregnant were sacrificed by cervical dislocation.

[0037] Isolate and take out the embryos under aseptic conditions, and separate them in a sterile petri dish under a dissecting microscope to obtain nerve cells. Place them in a carbon dioxide incubator at 37°C and 5% carbon dioxide saturated humidity for 48 hours before performing group experiments.

[0038] After the nerve cells were cultured in the 96-well plate for 48 hours, 5 mg / ml of the fucoidan sulfate prepared in Example 1 were added to make the final concentrations of the fucoidan sulfate respectively 100 μg / ml, 200 μg / ml, and 300 μg / ml, 400μg / ml, a negative control group (blank), and a positive control group for nerve growth factor. Incubate for 48 hours at 37°C, 5% carbon dioxide saturated humidity.

[0039] The analysis results showed that fucoidan sulfate had obvious dedifferentiation effect...

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PUM

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Abstract

The invention discloses a fucose sulfate or a compound whose active component is fucose sulfate, in the drug preparation on the treatment of nerve retrograde disease and cognition damage. And the invention discloses the relative application. The inventive fucose sulfate is mainly from marine invertebrate animal as sea-tangle, sea cucumber or the like, with wide resource, easy production, batch production, and wide application.

Description

Technical field [0001] The invention belongs to the field of biomedicine. In particular, it relates to the application of fucoidan sulfate in the preparation of drugs or health care products for preventing and treating neurodegenerative diseases. Background technique [0002] Nervous system degenerative diseases are diseases that seriously endanger people's health. Currently, about 1 billion people in the world are sick due to damage to the nervous system. Neurodegenerative diseases include a variety of diseases, such as common Alzheimer’s disease (AD), Parkinson’s disease (PD), etc. Most of these diseases develop progressively and are a type of disease whose etiology and pathogenesis are still unclear. Its pathological characteristics are atrophy and neuron loss of nerve nuclei with specific functions. Clinically, there are no effective measures to control the progression of this type of disease, and patients will eventually lose their ability to live or even die. [00...

Claims

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Application Information

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IPC IPC(8): A61K31/737A61K9/08A61K9/20A61K9/48A61K8/73A61P21/00A61P25/14A61P25/16A61P25/28A23L1/30A23L2/38C08B37/00
Inventor 吉爱国宋淑亮宋迪
Owner SHANDONG UNIV
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