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Method for preparing hepatitis B virus resisting maggot protein powder and its maggot oil and fatty acid

A technology of fatty acid and fly maggot oil, which is applied in the field of bioengineering, can solve the problems of rarely developing health care products and general functions, and achieve good inhibitory and therapeutic effects, easy absorption, and stable quality

Inactive Publication Date: 2007-10-24
沈荣法 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fatty acids produced from vegetable oils have general functions and have a certain health care effect on the human cardiovascular system, but they can only be used as food and are rarely developed as health products.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Use the fly maggots cultivated by the above breeding method as raw materials, wash the fly maggots with water, dry them at a temperature of 40°C at a vacuum of 0.01Mpa until the water content in the fly maggots is 10%, pulverize them, add petroleum ether twice its weight, and dry them at 5 Extract at ℃ for 4 hours, separate the solid and liquid phases, and evaporate the liquid phase at 30 °C below the vacuum of 0.01Mpa to recover petroleum ether to obtain fly maggot oil and fly maggot protein powder; In the buffer solution of sodium pH6.0, add porcine pancreatic neutral lipase to reach 180 international units of enzyme activity per milliliter; The ratio is 1:0.8, keep at 38°C, stir and react for 2.0 hours, stop the reaction, separate statically, separate the oil phase, wash with equal volume of water 3 times, and dry the oil phase at 40°C under a vacuum of 0.01Mpa to obtain fly maggots fatty acid.

Embodiment 2

[0017] Using the fly maggots cultivated by the above breeding method as raw materials, wash the fly maggots with water, dry them at a vacuum degree of 0.003Mpa and a temperature of 35°C until the water content in the fly maggots is 13%, pulverize them, add 8 times its weight of ethane, and dry them at 40 Extract at ℃ for 2 hours, separate the solid and liquid phases, and evaporate the liquid phase at 35 °C below the vacuum of 0.003Mpa to recover ethane to obtain fly maggot oil and fly maggot protein powder; In the pH8.0 buffer solution, add yeast neutral lipase to reach 200 international units of enzyme activity per milliliter; add fly maggot oil to the buffer solution equipped with yeast neutral lipase, and the volume ratio of buffer solution to fly maggot oil is 1:1.0, kept at 40°C, stirred for 3.5 hours, stopped the reaction, separated statically, separated the oil phase, washed 4 times with an equal volume of water, and evaporated the oil phase to dryness at 35°C under a va...

Embodiment 3

[0019] Use the fly maggots cultivated by the above breeding method as raw materials, wash the fly maggots with water, dry them at a temperature of 38°C under a vacuum of 0.008Mpa until the water content in the fly maggots is 15%, crush them, and add petroleum ether and ethane 7 times its weight The mixed solvent of petroleum ether and ethane is 1:1, leaching at 20°C for 4 hours, separating the solid and liquid phases, and the liquid phase is evaporated at 40°C under a vacuum of 0.008Mpa to recover the mixture of petroleum ether and ethane Solvent to obtain fly maggot oil and fly maggot protein powder; in the sodium monohydrogen phosphate and sodium dihydrogen phosphate pH7.0 buffer solution configured with water, add bacterial neutral lipase to reach 195 international units of enzyme activity per milliliter; Add fly maggot oil to the buffer solution equipped with bacterial neutral lipase, the volume ratio of buffer solution and fly maggot oil is 1:1.3, keep at 39°C, stir and re...

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PUM

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Abstract

The invention discloses an anti-hepatitis virus fly-blown protein powder and the method for preparing its fly-blown oil and fatty acid. The method comprises: washing fly-blown cultured with specific method with water, drying at vacuum degree being smaller 0. 01MPa until water content is below 15%, disintegrating, adding organic solvent, extracting, separating solid-liquid phase, evaporating liquid phase and recovering solvent, getting fly-blown oil, fly-blown protein powder; adding neutral lipase into buffer solution with pH being 6. 0-8. 0 and prepared with disodium hydrogen phosphate and acid sodium phosphate and water, reaching 180-200 enzyme activity per ml; adding fly-blown oil into buffer solution with neutral lipase, the volumetric ratio between buffer solution and fly-blown oil is 1: 0. 8- 1. 3, stirring, stopping reaction, stewing, separating oil phase, washing, evaporating oil phase to dryness and getting fly-blown fatty acid. The product is characterized by non-toxicity, stable quality, easy absorption, and good effect for preventing and treating hepatitis.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a preparation method of fly maggot protein powder capable of resisting hepatitis B virus and fly maggot oil and fatty acid. Background technique [0002] At present, fly maggots are used as raw materials for high-protein feed, and fly maggots are not extracted from fly maggot oil, and fly maggots are dried and powdered to be sold as feed. [0003] The production technology of fatty acid is first to use marine fish oil as raw material, hydrolyze, and then separate and purify to obtain mixed fatty acid, wherein the content of unsaturated fatty acid is relatively high. Another technique is to use vegetable oil as raw material, undergo hydrolysis, and then separate and purify to obtain mixed fatty acids. The fatty acids produced from marine fish oil, because of their high content of unsaturated fatty acids, have the functions of softening blood vessels, enhancing ...

Claims

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Application Information

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IPC IPC(8): A61K35/64A61P1/16A61P31/20C12P7/40C07K1/14C11B1/10
Inventor 沈荣法陈炳香杨心辉
Owner 沈荣法
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