High temperature revulsion method for improving lentinan output

A technology of lentinan and yield, which is applied in the field of microbial fermentation, can solve the problem that the yield of lentinan is not very high, and achieve the effects of short cycle, increased polysaccharide yield, and increased yield

Inactive Publication Date: 2007-12-05
重庆工学院
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Problems solved by technology

[0004] However, the existing liquid submerged fermentation culture breaks the natural growth and development rules of microorganisms, especially the metabolites with important physiological activities, such as lentinan, which are generally affected by various environmental factors when the bacteria mature or even produce fruiting bodies. Stimulation only accumulates, so the yield of lentinan produced by the existing submerged liquid fermentation culture is not very high

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  • High temperature revulsion method for improving lentinan output

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Embodiment Construction

[0017] As shown in Figure 1, the innovation of the present invention is to increase the induction process of liquid culture in the process of fermenting lentinan. The technological process of fermenting the lentinan polysaccharide comprises the steps of inoculating the lentinan strain → liquid medium inoculation → liquid medium cultivation → liquid culture induction → continuous cultivation after induction → polysaccharide content determination and the like. in,

[0018] Inoculation of shiitake mushroom strains: Pick mycelium pieces from the original strains stored in the 4°C refrigerator and connect them on a fresh PDA plate, and culture them in a water-proof electric constant temperature incubator at 28°C for 2 days.

[0019] Liquid medium inoculation: Cut the activated plate bacteria into mycelium pieces with a diameter of 0.4 cm, inoculate in liquid medium, and inoculate one bottle for every 20 bacterial cakes. When inoculating, pay attention to the same inoculum size and...

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Abstract

The high temperature induction method for raising the yield of lentinan belongs to the field of microbial fermentation technology. During the liquid fermentation process of lentinus edodes, the cultured matter is induced, and that is, the lentinus edodes culturing liquid after being cultured for 3-5 days is taken out and induced at 40-50 deg.c for 8-12 min. The present invention makes it possible to obtain great amount of mycelium and its fermented product in relatively short time, and has broad application foreground in edible fungus production. Compared with traditional liquid lentinus edodes fermentation, the present invention has a 20-30 % lentinan yield.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a high-temperature induction method for increasing the yield of lentinan. Background technique [0002] Lentinus edodes is a fungus that can be used as both food and medicine. It is mild in nature and sweet in taste. Shiitake mushrooms contain protein, 19 kinds of amino acids, fat, unsaturated fatty acids, crude fiber, trace elements, ergosterol, thiamine, riboflavin, niacin, vitamin B 12 and vitamin C, etc. In addition, it is also rich in lentinan and lentinan. Malignant tumors such as esophageal cancer, gastric cancer, intestinal cancer and lung cancer all have obvious curative effects, and the tumor suppression rate reaches about 80%, which can improve human immunity. The structure of lentinan is a glucan with β-1,3-glucose residue as the main chain and β-1,6 glucose residue as the side chain. Lentinan has a wide range of pharmacological activitie...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/645
Inventor 王万能
Owner 重庆工学院
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