Method for simultaneously determining multiple organic acid content of fruit

An organic acid and fruit technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low efficiency and long time, and achieve the effect of protecting the chromatographic column, high accuracy and good reproducibility

Inactive Publication Date: 2008-01-09
FUDAN UNIV
View PDF0 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the determination of organic acids in fruits by high-performance liquid chromatography is simple, accurate, and reproducible, it can also quantitatively determine a variety of organic acids at the same time, but the time is too long, which reduces the efficiency

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for simultaneously determining multiple organic acid content of fruit
  • Method for simultaneously determining multiple organic acid content of fruit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] Below in conjunction with embodiment further illustrate the present invention.

[0031] 1. Preparation of reference solution:

[0032] Take oxalic acid, tartaric acid, citric acid, succinic acid, L-(-)-malic acid, fumaric acid, lactic acid, and vitamin C reagents with a purity of ≥99%, and prepare about 0.3 g of the above eight organic acids with the mobile phase water phase / L standard stock solution. According to the actual content of organic acids in the fruit and the response value of each organic acid at a certain ultraviolet detection wavelength, dilute a certain multiple to prepare a mixed standard solution containing the above 8 kinds of organic acids, and then dilute the above solutions into different concentration gradients, That is, the concentration of the above mixed standard solution is 0.2, 0.4, 0.6, 0.8, 1.0 times.

[0033] 2. Preparation of the solution to be tested:

[0034] Juicing method sample preparation: select crystal pears - peel - slice - sq...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
volumeaaaaaaaaaa
Login to view more

Abstract

The invention is a high performance liquid chromatography of determining diversofiform organic acid in the fruit simultaneity. It adopts liquid chromatograph including ultraviolet detector and chromatographic column of silica gel bonding in opposition. It takes organic solvent mobile phase A and aqueous solution mobile phase B of finite concentration pH value as gradient elution agent. The invention only single accomplishes the procession of quantitative detection of the primary organic acid in the fruit on the regular high performance liquid chromatography. The method of the invention is advanced, nicety, simple and convenience, the analyzable time can cut within 8min, improning the analytic efficiency greatly. At the same time, the method takes the Carrez reagent as decontaminant of fruit sample, this is the first time using the Carrez reagent to detect fruity organic acid.

Description

technical field [0001] The invention relates to a method for determining the content of organic acids in fruits. Using the specific chromatographic conditions of a common high-performance liquid chromatograph, the determination of oxalic acid, tartaric acid, vitamin C, malic acid, lactic acid, citric acid, fumaric acid, and succinic acid in fruits can be completed. Determination of organic acids, etc., to obtain accurate results. Background technique [0002] The analysis methods of organic acids in fruits mainly include: acid-base titration, thin layer chromatography, gas chromatography (GC), ion chromatography (HPIC) and high performance liquid chromatography. Acid-base titration can only measure the total acid content, and the operation is cumbersome; thin-layer chromatography has poor quantitative accuracy; gas chromatography often requires derivation steps because the acid has a high boiling point and is not suitable for gasification, with large errors and cumbersome me...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/26
Inventor 张晋芬袁冰徐华龙沈伟
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap