Bindwood sapogenin and its preparation method and use

A technology of hedera saponin and black cumin seeds, applied in the field of hedera saponin and its preparation and application

Inactive Publication Date: 2008-02-20
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the research on Nigella tumefaciens is limited to Hao Haifeng, Liu Yuming et al. who have isolated 14 compounds from the seeds of Nigella tumefaciens, and Tazawa et al. have investigated the antitumor activity of hedera saponins. Its lipid-lowering and hypoglycemic activity has not been reported yet

Method used

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  • Bindwood sapogenin and its preparation method and use
  • Bindwood sapogenin and its preparation method and use
  • Bindwood sapogenin and its preparation method and use

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Take 10KG of dried Nigella nigella seeds, degrease them with petroleum ether, extract them sequentially with 95% and 50% ethanol, concentrate under reduced pressure to a non-alcoholic extract, suspend them in water, and use petroleum ether, ethyl acetate, n-butanol extraction, recovery n-butanol extract, concentrated under reduced pressure to extract;

[0026]Process the macroporous resin AB-8 according to the conventional method, after the extractum is dissolved in water, the liquid loading, the sample amount and the resin consumption ratio are 1: 3, water, 10-95% ethanol successively elutes the macroporous resin column, reduces Concentrate under reduced pressure, use thin-layer chromatography to combine components with similar components, mix 70-95% of the components with silica gel, evaporate the solvent with a rotary evaporator, dry, silica gel column chromatography, chloroform-methanol gradient elution, and combine the effluents solution, silica gel thin layer plat...

Embodiment 2

[0029] Take 10KG of dried Nigella nigella seeds, degrease them with petroleum ether, extract them sequentially with 95% and 50% ethanol, concentrate under reduced pressure to a non-alcoholic extract, suspend them in water, and use petroleum ether, ethyl acetate, n-butanol extraction, recovery n-butanol extract, concentrated under reduced pressure to extract;

[0030] Process the macroporous resin AB-8 according to the conventional method, after the extractum is dissolved in water, apply the liquid, the sample amount and the resin dosage ratio are 1: 30, and the macroporous resin column is eluted successively with water and 10-95% ethanol, reducing Concentrate under reduced pressure, use thin-layer chromatography to combine components with similar components, dissolve 70-95% of the components with methanol and water, apply the liquid, elute with methanol and water, detect by silica gel thin-layer plate chromatography, and combine the same fractions to obtain The crude product o...

Embodiment 3

[0033] Take 10KG of dried Nigella nigella seeds, degrease them with petroleum ether, extract them sequentially with 95% and 50% ethanol, concentrate under reduced pressure to a non-alcoholic extract, suspend them in water, and use petroleum ether, ethyl acetate, n-butanol extraction, recovery n-butanol extract, concentrated under reduced pressure to extract;

[0034] Process the macroporous resin AB-8 according to the conventional method, after the extractum is dissolved in water, the liquid loading, the sample amount and the resin consumption ratio are 1: 15, water, 10-95% ethanol successively elutes the macroporous resin column, reduces Concentrate under reduced pressure, use thin-layer chromatography to combine components with similar components, dissolve 70-95% of the components with methanol and water, apply the liquid, elute with methanol and water, detect by silica gel thin-layer plate chromatography, and combine the same fractions to obtain The crude product of the com...

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Abstract

The invention relates to a hederagenin and a preparation method and purposes thereof. The compound is that the hederagenin is distilled from Nigella glandulifera freyn by degreasing, leaching and macroporous resin absorption, a structure of the hederagenin is confirmed by using physico-chemical constants, spectra database and single crystal method of X-ray diffractometry, the distilled hederagenin is proved in vitro to have the effect of tyrosine protein phosphatase PTP1B retardant and purposes as anti-diabetes drugs and health care products, meanwhile, the hederagenin is also applicable to be combination of drugs made from the compound or derivatives of the compound.

Description

technical field [0001] The invention relates to a method for extracting hedera saponin from black cumin seeds of the vegetative medicine and its preparation method, and its medicinal use as a protein tyrosine phosphatase inhibitor for treating diabetes. The effect of the protein tyrosine phosphatase 1B (PTP1B) inhibitor that the extracted helexin has, and the application of the formed antidiabetic drug and health care products confirmed by in vitro experiments. Background technique [0002] The seeds of Nigella spp. are the dried and mature seeds of Nigella spp., a plant of the family Ranunculaceae, in a triangular oval shape with a length of 2.5-3mm and a width of about 1.5mm. The surface is black and rough, the top is narrow and pointed, the bottom is slightly blunt, and there are irregular protrusions. The texture is hard, the section is gray and oily. The gas is slightly fragrant, and the taste is pungent. It can stimulate digestion, dispel wind and relieve pain, invi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00A61K31/56A61K36/71A61P3/10
Inventor 信学雷阿吉艾克拜尔·艾萨
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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