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Solid composite micro-organism micro-balloon for organic contaminant biodegradation and method for preparing the same

A technology for compounding microorganisms and organic pollutants, applied in the field of solid composite microbial microspheres and its preparation, to achieve the effects of slowing migration, avoiding diffusion, and good biocompatibility

Inactive Publication Date: 2008-03-05
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the problem of contact between the substrate and the embedded microorganisms existing in the existing microbial embedding preparations, the purpose of the present invention is to provide a solid composite microbial microsphere for the biodegradation of organic pollutants and a preparation method thereof. The porous microbial embedding carrier can enrich organic pollutants and promote the growth of embedded microorganisms, so as to achieve the purpose of high efficiency and fast biodegradation of organic pollutants

Method used

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  • Solid composite micro-organism micro-balloon for organic contaminant biodegradation and method for preparing the same
  • Solid composite micro-organism micro-balloon for organic contaminant biodegradation and method for preparing the same
  • Solid composite micro-organism micro-balloon for organic contaminant biodegradation and method for preparing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Surface culture: Phanerochaetechrysosoporium (purchased from China General Microorganism Culture Collection Center) was used as the fungus for the degradation of petroleum hydrocarbons. Prepare potato plane culture medium (PDA), inoculate Phanerochaete chrysosporium on the PDA plane culture medium, cultivate at 28°C for 6 days, and preserve for later use;

[0033] (2) Rinse the cultivated PDA plate with sterilized 20mMPBS (pH=7.0) to prepare a spore liquid with a concentration of 10 spores / ml;

[0034] (3) Sodium alginate-adsorbent solution is prepared, wherein the concentration of sodium alginate is 2%, and the adsorbent is selected to have a lignin biopolymer with strong absorption to phenanthrene, and the content is 5%;

[0035] (4) At normal temperature, uniformly disperse 800 mesh calcium carbonate particles in the solution described in (3), the concentration of calcium carbonate is 5%; then add the spore suspension obtained in (2) to the solution to make the s...

Embodiment 2

[0039] After the solid composite microbial microspheres obtained in Example 1 were placed at 30° C. for 24 hours, the free spores (sample one), the non-porous sodium alginate-embedded spores (sample two) and the samples obtained in Example 1 were compared by weighing method. The fungal biomass of the prepared solid composite microbial microspheres, the results (see Figure 3) show that the prepared solid composite microbial microspheres of the present invention can well promote the growth of fungi, and the 24h fungal biomass is respectively sample one and sample two 28.6 and 2.5 times. After culturing for 48 hours, the fungal morphology in the solid composite microbial microspheres prepared in Example 1 was observed under a microscope (as shown in FIG. 4 ).

Embodiment 3

[0041] Adsorption Kinetic Experiment of Organic Pollutant Phenanthrene

[0042] 0.4 g (wet weight) of solid composite microbial microspheres obtained in Example 1 was placed in 20 ml containing 5 mg / L phenanthrene, 200 mg / LTween80 and 200 mg / L NaN 3 In the 5mM PBS (pH=7.0) buffer solution (inhibiting the activity of embedded microorganisms), the adsorption kinetics of the carrier to the organic pollutant phenanthrene was investigated, wherein the contrast was the liquid phase system without adding any adsorbent, and according to the patent 200510130675.9 ( CN) made in only super large pores, does not contain the carrier of organic pollutant class adsorbent. Result (see Fig. 5) in the solution that adds the composite microorganism microsphere prepared in embodiment 1, residual phenanthrene content is 12% of initial phenanthrene content when 10h, illustrates that the carrier prepared by the present invention has good adsorption capacity to phenanthrene, However, the content of ...

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Abstract

The present invention discloses one kind of solid composite microbe microsphere for biodegrading organic pollutant and its preparation process. The macroporous microbe-embedding microsphere is prepared with sodium alginate as the embedding material, solid calcium carbonate as the pore creating agent, and adsorbent capable of adsorbing organic pollutant and thallus for degrading other pollutant added into the embedding suspension. It combines adsorption on the pollutant and biodegradation, and has high pollutant eliminating efficiency and high pollutant eliminating speed.

Description

technical field [0001] The invention belongs to a biodegradation method for organic pollution, in particular to a solid composite microbial microsphere for biodegradation of organic pollutants and a preparation method thereof. Background technique [0002] The use of microorganisms to biodegrade is an effective method to control the environmental pollution of organic pollutants. However, due to the toxicity of organic pollutants, it is difficult for microorganisms to grow effectively in the biological treatment of organic pollutants, resulting in low degradation efficiency. Taking the treatment of petroleum hydrocarbon-contaminated soil as an example, petroleum hydrocarbons have complex compositions, strong biological toxicity, and low bioavailability. Therefore, it is difficult for external bacteria to accumulate biomass in harsh petroleum hydrocarbon-contaminated soils, and the degradation conversion efficiency is low. At present, foreign countries have developed microbial...

Claims

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Application Information

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IPC IPC(8): C12N11/04C02F3/34C09C1/10
Inventor 张坤刘铮徐圆圆花秀夫张敏莲王剑楠
Owner CHINA PETROLEUM & CHEM CORP
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